Human tissues were labeled with the following antibodies: mouse anti-αSMA (clone 1A4, Dako, Carpineria, CA), rabbit anti-periostin (Abcam, Cambridge, ENG), mouse anti-CK19 (Dako clone RCK-108), mouse anti-CD68 (Dako clone KP1), and mouse anti-CD163 (Novocastra/Leica, New Castle Upon Tyne, ENG). Mouse tissues were labeled with the same αSMA and periostin antibodies and with F4/80 (clone A3-1, AbD Serotec, Raleigh, NC). Fibrillar collagen I was detected by Gomori trichrome (Newcomer Supply, Middleton, WI). Double immunofluorescence was performed using TSA-Plus kits (Perkin Elmer LAS, Boston, MA) and counterstained with Toto3 (Molecular Probes, Eugene, OR). For double immunofluorescence with same species antibodies, slides were boiled after the first antibody’s TSA reaction, then labeling was repeated with the second primary antibody. Controls always included slides which lacked either the first or second primary antibody to validate that boiling removed all of the first antibody.
IPS-1 cells were labeled with CellTracker Green CMFDA (Molecular Probes, Eugene OR) and RAW264.7 and BMDM-WT cells were labeled with CellTracker Red CMTPX (Molecular Probes) as per manufacturer’s instructions.
IPS-1 cells were labeled with CellTracker Green CMFDA (Molecular Probes, Eugene OR) and RAW264.7 and BMDM-WT cells were labeled with CellTracker Red CMTPX (Molecular Probes) as per manufacturer’s instructions.