Epifluorescence microscopy was performed using a Leica THUNDER Imager 3D cell culture epifluorescence microscope equipped with a Leica LED8 multi-LED light source and sCMOS camera (Leica DFC9000 GTC). All epifluorescence images were acquired without THUNDER computational clearing and are displayed with instrument noise subtracted but without background subtraction. See Table S6 for details on the objective, excitation wavelengths and filters used for each experiment.
Thunder imager 3d cell culture epifluorescence microscope
Manufactured by Leica
The THUNDER Imager 3D cell culture epifluorescence microscope is a high-performance imaging system designed for the visualization and analysis of three-dimensional cellular structures. The microscope utilizes advanced epifluorescence technology to capture detailed images of samples in a 3D environment.
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2 protocols using thunder imager 3d cell culture epifluorescence microscope
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Epifluorescence Microscopy Protocols
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Confocal and Epifluorescence Microscopy Protocols
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Confocal microscopy was performed using a Zeiss LSM 800 inverted confocal microscope or a Zeiss LSM 880 with Fast Airyscan upright confocal microscope. All confocal images are displayed without background subtraction. See Table S5 for details on the microscope, objective, excitation lasers, beam splitters, emission bandpass filters used for each experiment.
Epifluorescence microscopy. Epifluorescence microscopy was performed using a Leica THUNDER Imager 3D cell culture epifluorescence microscope equipped with a Leica LED8 multi-LED light source and sCMOS camera (Leica DFC9000 GTC). All epifluorescence images are displayed with instrument noise subtracted but without background subtraction. See Table S6 for details on the objective, excitation wavelengths, and filters used for each experiment.
Epifluorescence microscopy. Epifluorescence microscopy was performed using a Leica THUNDER Imager 3D cell culture epifluorescence microscope equipped with a Leica LED8 multi-LED light source and sCMOS camera (Leica DFC9000 GTC). All epifluorescence images are displayed with instrument noise subtracted but without background subtraction. See Table S6 for details on the objective, excitation wavelengths, and filters used for each experiment.
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