Gallium nitrate (Ga(NO3)3.H2O, 99.9%) was supplied by Alfa Aesar, UK. Ferric nitrate (Fe(NO3)3.9H2O, 99%), carboxymethyl cellulose (CMC, 250 kDa, D.S.=0.9), pectin (from citrus peel, galacturonic acid ≥74.0 %), cellulase from Aspergillus niger (>0.8 U/mg), pectinase from Aspergillus niger (>1.0 U/mg), alginate lyase (≥10,000 U/g), Phosphate-buffered saline (PBS) and resazurin were all purchased from Sigma Aldrich, UK. Alginic acid sodium salt was purchased from Fisher Scientific UK. Sodium acetate buffer (0.2 M, pH 7) was prepared from sodium hydroxide (CertiFied AR grade, Fisher Scientific, UK) and glacial acetic acid (Sigma-Aldrich, UK). Human Dermal Neonatal Fibroblasts (HDNFs) were purchased from the Coriell Institute for Medical Research, Camden, New Jersey, USA, Dulbecco's Modified Eagle Medium (DMEM) high glucose was supplied by Lonza, foetal bovine serum (FBS), penicillin/streptomycin and TrypLE Express were purchased from Gibco. Dimethyl sulfoxide (DMSO, laboratory grade) was purchased from Fisher Scientific, UK. All water used was deionised water. All chemicals and materials were used as received without further purification. All microbiological media were purchased from Fisher Scientific (UK).
Dulbecco s modified eagle medium dmem high glucose
Manufactured by Lonza
Sourced in Switzerland
Dulbecco's Modified Eagle Medium (DMEM) high glucose is a cell culture medium formulation. It provides essential nutrients and growth factors to support the in vitro cultivation of various cell types.
Automatically generated - may contain errors
Lab products found in correlation
Rhodamine phalloidin, by Thermo Fisher Scientific (3 mentions)
Sodium tripolyphosphate tpp, by Merck Group (2 mentions)
Peitc standard, by Santa Cruz Biotechnology (2 mentions)
Low molecular weight chitosan cs, by Merck Group (2 mentions)
Mem non essential amino acid solution, by Merck Group (2 mentions)
Penicillin streptomycin mixture, by Lonza (2 mentions)
Fetal bovine serum (fbs), by Biowest (2 mentions)
Fluoroshield mounting medium with dapi, by Abcam (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (2 mentions)
Ferric nitrate, by Merck Group (1 mentions)
Sodium acetate buffer, by Thermo Fisher Scientific (1 mentions)
Pectin, by Merck Group (1 mentions)
Resazurin, by Merck Group (1 mentions)
Carboxymethylcellulose, by Merck Group (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Tryple express, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Glacial acetic acid, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
5 protocols using dulbecco s modified eagle medium dmem high glucose
1
Biomaterial Synthesis and Characterization
2
Chitosan-TPP Nanoparticle Preparation
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Low molecular weight chitosan (CS) was obtained from Sigma-Aldrich (St. Louis, USA) and possessed a deacetylation degree between 75 and 85% and a molecular weight of 107 kDa. Sodium Tripolyphosphate (TPP) and MEM non-essential amino acid solution were supplied by Sigma-Aldrich (Merck, Darmstadt, Germany). Dulbecco’s Modified Eagle Medium (DMEM) high glucose and Penicillin-Streptomycin mixture were obtained from Lonza (Basel, Switzerland). Fetal bovine serum (FBS) was purchased from Biowest (Nuaillé, France). All the reagents were used as received without further purification. PEITC standard was supplied by Santa Cruz Biotechnology Inc. (Dallas, Texas, USA). The olive oil was an extra virgin, purchased in a local store. All the other reagents were of analytical grade and used without further purification. Ultrapure water was used to prepare all the solutions.
3
Chitosan Nanoparticle Synthesis and Characterization
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Low molecular weight chitosan (CS) was obtained from Sigma-Aldrich (St. Louis, USA) and possessed a deacetylation degree between 75 and 85% and a molecular weight of 107 kDa. Sodium Tripolyphosphate (TPP) and MEM non-essential amino acid solution were supplied by Sigma-Aldrich (Merck, Darmstadt, Germany). Dulbecco's Modified Eagle Medium (DMEM) high glucose and Penicillin-Streptomycin mixture were obtained from Lonza (Basel, Switzerland). Fetal bovine serum (FBS) was purchased from Biowest (Nuaillé, France). All the reagents were used as received without further purification. PEITC standard was supplied by Santa Cruz Biotechnology Inc. (Dallas, Texas, USA). The olive oil was an extra virgin, purchased in a local store. All the other reagents were of analytical grade and used without further purification. Ultrapure water was used to prepare all the solutions.
4
Fabrication of PLGA-collagen scaffolds
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PLGA with molar ratio of 50/50 and inherent viscosity of 1.05 dL/g was purchased from Purac (Gorinchem, Netherlands). Tetrafluoroethylene (TFE), Kolliphor® P188, 4-morpholineethanesulfonic acid (MES), EDC, and NHS were purchased from Sigma Aldrich (St. Louis, MO, USA). Chloroform was purchased from Fisher Scientific (Waltham, MA, USA). Collagen type I was purchased from Merck Millipore (Darmstadt, Germany). Fluoroshield mounting medium with DAPI was purchased from Abcam (Cambridge, UK). HDFs were purchased from Life Technologies (Fisher Scientific, Waltham, MA, USA). High glucose Dulbecco’s Modified Eagle Medium (DMEM) and PBS were purchased from Lonza (Basel, Switzerland). Fetal bovine serum (FBS) and antibiotic-antimycotic were purchased from Gibco (Fisher Scientific, Waltham, MA, USA).
5
Epithelial-Mesenchymal Transition Markers
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High glucose Dulbecco’s Modified Eagle Medium (DMEM) and RPMI-1640 were purchased from Lonza (Basel, Switzerland). Fetal bovine serum (FBS) and antibiotic-antimycotic were purchased from Gibco (Fisher Scientific, Waltham, MA, USA). E-Cadherin, N-Cadherin, vitronectin, fibronectin, SNAI1 antibodies were from Cell Signaling Technology, USA; Alexa-fluor Goat anti-rabbit 594, Alexa-fluor Goat anti-mouse 488, Alexa Fluor 488 Phalloidin and Rhodamine phalloidin were from Thermo Fisher Scientific, USA. Fluoroshield mounting medium with DAPI was purchased from Abcam (Cambridge, UK). Unless otherwise indicated, all reagents were obtained from Sigma-Aldrich.
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