Three-micron-thick formalin-fixed paraffin-embedded (FFPE) tumor samples were stained either with hematoxylin and eosin and processed for IHC. In this case, IHC was performed with an automatic stainer (BOND III, Leica Microsystems, Wetzlar, Germany), and by using the following antibodies, according to the manufacturer’s instructions: anti-phospho-Histone H3 (pHH3) Rabbit polyclonal Ab (1:100 dilution, Cell Signaling Technology), anti-Caspase3 Rabbit (1:50 diluition, Cell Signaling Technology) and anti-mouse CD31 Rat mAb (clone SZ31, dilution 1:20, DIANOVA GmbH-Hamburg Germany).
Anti phospho histone h3 phh3 rabbit polyclonal ab
Manufactured by Cell Signaling Technology
Sourced in Germany, United States
Anti-phospho-Histone H3 (pHH3) Rabbit polyclonal Ab is a laboratory reagent that detects the phosphorylation of histone H3 at serine 10 and serine 28. It is commonly used as a marker for cell proliferation and mitosis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti phospho histone h3 phh3 rabbit polyclonal ab
1
Immunohistochemistry of FFPE Tumor Samples
2
Immunohistochemical Profiling of Tumor Samples
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Three-micron-thick formalin-fixed, paraffin-embedded tumor samples were either stained with hematoxylin and eosin or processed for IHC. In this case, IHC was performed by using an automatic stainer BOND III (Leica Microsystems, Wetzlar, Germany) and by using the following antibodies according to the manufacturer's instructions: anti-ACC rabbit mAb (clone C83B10, dilution 1:100), anti-FAS rabbit mAb (clone C20G5, dilution 1:100), anti-phospho-histone H3 (pHH3) rabbit polyclonal Ab (dilution 1:100), all from Cell Signaling Technology Danvers, Massachusetts, USA; anti-GLS rabbit mAb (clone EP7212, dilution 1:200), anti-PHGDH mouse mAb (clone Ab57030, dilution 1:100) both from Abcam, Cambridge, UK, anti-MCT1 rabbit polyclonal Ab (dilution 1:50; Millipore, Burlington, MA, USA), anti-MCT4 rabbit polyclonal Ab (dilution 1:300; Santa Cruz Biotechnology, Dallas, TX, USA), and anti-mouse CD31 rat mAb (clone SZ31, dilution 1:20; DIANOVA GmbH-Hamburg, Germany).
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