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Nattl tlcl3

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[natTl]TlCl3 is a chemical compound that consists of thallium chloride. It is a solid, crystalline material. The core function of this product is to serve as a laboratory reagent for various scientific applications.

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Lab products found in correlation

Gelred nucleic acid stain, by Biotium (2 mentions) Pbr322 plasmid dna, by New England Biolabs (2 mentions) 201tl tlcl3, by New England Biolabs (2 mentions) Geldoc itts2 310 imager system, by Bio-Rad (2 mentions) Gelcam 310, by Bio-Rad (2 mentions)

2 protocols using nattl tlcl3

1

Radiolabeling Plasmid DNA with Thallium-201

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pBR322 DNA plasmid (New England Biolabs, UK) in PBS (100 ng, 20 µL) was incubated with 0.5 MBq (8 µL) [201Tl]TlCl3 for upto144 h. [201Tl] TlCl3, originally formed using chloramine-T (method 6), was neutralised with Na2CO3 (0.1 M). Controls included untreated plasmid in PBS and equivalent amounts of non-radioactive [natTl]TlCl3 (Sigma). After treatment, plasmid (50 ng in PBS) was mixed with 6× loading dye (16 µL total volume), loaded onto a 0.8% agarose gel containing 10 µL GelRed Nucleic Acid stain (Biotium, USA) and run at 100 V (400 mA, 50 W) for 40 min. Gels, imaged using a GelDoc-ItTS2 310 Imager system (BioRad, UK) coupled with a Benchtop UV transilluminator (UVP) and GelCam 310, were analysed by ImageJ, measuring supercoiled (intact DNA), relaxed circular (single strand breaks) and linear band (double strand breaks) percentages within a lane (n = 3–12) [33 (link)].
Rigby A., Blower J.E., Blower P.J., Terry S.Y, & Abbate V. (2021). Targeted Auger electron-emitter therapy: Radiochemical approaches for thallium-201 radiopharmaceuticals. Nuclear medicine and biology, 98-99, 1-7.
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2

Radiolabeling Plasmid DNA with Thallium-201

Check if the same lab product or an alternative is used in the 5 most similar protocols
pBR322 DNA plasmid (New England Biolabs, UK) in PBS (100 ng, 20 µL) was incubated with 0.5 MBq (8 µL) [201Tl]TlCl3 for upto144 h. [201Tl] TlCl3, originally formed using chloramine-T (method 6), was neutralised with Na2CO3 (0.1 M). Controls included untreated plasmid in PBS and equivalent amounts of non-radioactive [natTl]TlCl3 (Sigma). After treatment, plasmid (50 ng in PBS) was mixed with 6× loading dye (16 µL total volume), loaded onto a 0.8% agarose gel containing 10 µL GelRed Nucleic Acid stain (Biotium, USA) and run at 100 V (400 mA, 50 W) for 40 min. Gels, imaged using a GelDoc-ItTS2 310 Imager system (BioRad, UK) coupled with a Benchtop UV transilluminator (UVP) and GelCam 310, were analysed by ImageJ, measuring supercoiled (intact DNA), relaxed circular (single strand breaks) and linear band (double strand breaks) percentages within a lane (n = 3–12) [33 (link)].
Rigby A., Blower J.E., Blower P.J., Terry S.Y, & Abbate V. (2021). Targeted Auger electron-emitter therapy: Radiochemical approaches for thallium-201 radiopharmaceuticals. Nuclear medicine and biology, 98-99, 1-7.
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