Toluene 2 4 diisocyanate

The Toluene 2,4-diisocyanate was purchased from Junsei Chemical Co., dimethyl sulfoxide (DMSO), HC-030031, protease inhibitor cocktail, phosphatase inhibitor cocktail 2/3, and sodium dodecyl sulfate (SDS) were purchased from Sigma-Aldrich Chemical (St. Louis, MO, USA). Antibody against anti-DUSP6 (ab238512) was purchased from Abcam (Cambridge, MA, USA). Antibodies against anti-p-ERK1/2 (9101S), and anti-p-p53 (9284S) were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against anti-p53 (sc-126, anti-ERK1/2 (sc-514302), anti-Caspase3 (sc-7148), anti-Bax (sc-526), anti-Bcl2 (sc-7382), β-actin (sc-47778), HRP-conjugated anti-rabbit IgG (sc-2357), HRP-conjugated anti-mouse IgG (sc-516102), and mouse anti-rabbit IgG-CFL 488 (sc-516248) were purchased from Santa Cruz biotechnology (Santa Cruz, CA, USA). RNeasy Mini Kit was from Qiagen (Qiagen, Hilden, Germany), and the lactate dehydrogenase (LDH) assay kit was from DYNEBIO (Seoul, Korea).

Toluene 2,4-diisocyanate (TDI) was purchased from Junsei Chemical Co. (Tokyo, Japan). Triton X-100, sodium dodecyl sulfate (SDS), phosphatase inhibitor cocktail 2/3, protease inhibitor cocktail, 4,6-diamidino-2-phenylindole dihydrochloride (DAPI), and dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich Chemical (St. Louis, MO, USA). SB-431542 was purchased from MedChemExpress USA (Middlesex, NJ, USA). Antibodies against anti-TGF-β1(21898-1), anti-E-cadherin (60335-1-Ig), and anti-N-cadherin (66219-1-Ig) were purchased from Proteintech (Rosemont, IL, USA). Antibodies targeting anti-p-SMAD2/3 (8828S), anti-SMAD2/3(8685S), anti-α-SMA (19245S), and anti-mouse Alexa 488 (4408S) were purchased from Cell Signaling Technology (Beverly, MA, USA). The fibronectin antibody (MA5-11981) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Anti-vimentin (sc-6260), anti-β-actin (sc-47778), HRP-conjugated anti-rabbit IgG (sc-2357), anti-rabbit CFL 488 (sc-516248), and HRP-conjugated anti-mouse IgG (sc-516102) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, Dallas, CA, USA).