The Agilent Human (8*60K) V21.0 miRNA microarrays (Agilent, Santa Clara, CA, United States) were performed using a Gene Expression Hybridization Kit (Agilent’s miRNA Complete Labeling and Hyb Kit-p/n5190-0456) according to the manufacturer’s instructions. Slides were washed in staining dishes with a Gene Expression Wash Buffer Kit (Agilent, Santa Clara, CA, United States) and scanned by an Agilent Microarray Scanner with default settings according to the manufacturer’s instructions. The data was extracted using an Agilent Feature Extraction (AFE) software (version 10.7.1.1), and the raw data were normalized by Quantile algorithm using Gene Spring Software 12.6 (Agilent Technologies). Differentially expressed miRNAs were identified through the filtering of fold-change (>2), p value (<0.05) and FDR (<0.05) using R software (version 3.2.3) with the samr package. Also, the differentially expressed miRNAs should not have probes with flag A in at least one group.
Agilent human 8 60k v21.0 mirna microarrays
Manufactured by Agilent Technologies
Sourced in United States
The Agilent Human (8*60K) V21.0 miRNA microarrays are a tool designed for the measurement and analysis of microRNA expression levels. These microarrays contain probes that target human microRNA sequences.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using agilent human 8 60k v21.0 mirna microarrays
1
Agilent miRNA Microarray Differential Expression
2
Agilent miRNA Microarray Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Agilent Human (8*60K) V21.0 miRNA microarrays (Agilent, Santa Clara, CA, USA) were performed using a Gene Expression Hybridization Kit (Agilent's miRNA Complete Labeling and Hyb Kit-p/n5190-0456) according to the manufacturer's instructions. Slides were washed in staining dishes with a Gene Expression Wash Buffer Kit (Agilent, Santa Clara, CA, USA) and scanned by an Agilent Microarray Scanner with default settings according to the manufacturer's instructions. The data was extracted using an Agilent Feature Extraction (AFE) software (version 10.7.1.1), and the raw data were normalized by Quantile algorithm using Gene Spring Software 12.6 (Agilent Technologies). Differentially expressed miRNAs were identi ed through fold-change (> 2) and P value (< 0.05) ltering using R software (version 3.2.3) with the samr package.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!