Heat inactivated fbs

Manufactured by Capricorn

Heat-inactivated Fetal Bovine Serum (FBS) is a common cell culture supplement derived from the blood of bovine fetuses. The serum is heat-treated to inactivate any potential biological contaminants. This process does not alter the core function of the FBS, which is to provide growth factors, nutrients, and other components necessary to support the growth and proliferation of cells in in vitro cell culture applications.

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Lab products found in correlation

Streptomycin, by Thermo Fisher Scientific (3 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Magnisort mouse nk cell enrichment kit, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by GE Healthcare (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Interleukin 4 (il 4), by Merck Group (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) L glutamine, by Merck Group (1 mentions) Ifn γ, by BD (1 mentions)

2 protocols using heat inactivated fbs

Isolation and Culture of Murine NK Cells

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NK cells were isolated from the splenocytes of BALB/c mice using the MagniSort Mouse NK cell Enrichment Kit (eBioscience), in accordance with the manufacturer's instructions. NK cells were cultured in RPMI 1640 medium (GE Healthcare Life Sciences) supplemented with 2.05 mM L-glutamine, 10% (v/v) heat-inactivated FBS (Capricorn Scientific) and 1% (v/v) penicillin-streptomycin (10,000 U/mL penicillin, 10,000 μg/mL streptomycin; Gibco).

Oh E.Y., & Lee S. (2021). Natural Killer Cell Activation by Weissella cibaria JW15 Isolated from Kimchi. Journal of Bacteriology and Virology, 51(2), 62-73.

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Bone Marrow Macrophage Polarization

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Bone marrow cells were isolated from tibia and femur, and cultured for 13 days in RPMI-1640 medium supplemented with 300 mg/ml L-glutamine (Sigma-Aldrich), 100 mg/ml streptomycin (ThermoFisher Scientific), 100 units/ml penicillin, 20% (v/v) heat-inactivated FBS (Capricorn Scientific) and 30% L929-conditioned medium. Subsequently, BMDMs were harvested and plated at a concentration of 10 6 cells/ml in RPMI-based complete medium. Next, BMDMs were stimulated with either 10 ng/ml LPS (Sigma-Aldrich) and 100 units/ml IFN-g (BD Biosciences) or 20ng/ml IL-4 (Sigma-Aldrich). Macrophage polarization was assessed by ELISA and flow cytometry as described before (Ying et al., 2013) (link).

Geeraerts X., Fernández-Garcia J., Hartmann F.J., de Goede K.E., Martens L., Elkrim Y., Debraekeleer A., Stijlemans B., Vandekeere A., Rinaldi G., De Rycke R., Planque M., Broekaert D., Meinster E., Clappaert E., Bardet P., Murgaski A., Gysemans C., Nana F.A., Saeys Y., Bendall S.C., Laoui D., Van den Bossche J., Fendt S.M, & Van Ginderachter J.A. (2021). Macrophages are metabolically heterogeneous within the tumor microenvironment. Cell reports, 37(13).

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