C57BL/6j (B6) mice were purchased from Japan SLC, Inc, or Japan CLEA, Inc. Five- to seven-week-old mice were subcutaneously inoculated with 104 PFU of JEV diluted in EMEM containing 2% FCS. Mock infected mice were inoculated with supernatant of cultured BHK cells. Mice were weighed daily, and survivals were recorded for 21 days.
C57bl 6j b6 mice
Manufactured by Japan SLC
Sourced in Japan
C57BL/6J (B6) mice are a widely used inbred mouse strain that serve as a standard laboratory model. They are genetically homogeneous and exhibit consistent physiological and behavioral characteristics.
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Lab products found in correlation
Il 19 mice b6 background, by Regeneron Pharmaceuticals (2 mentions)
Crf 1, by Oriental Yeast (1 mentions)
129s6 svev stat1tm1rds, by Taconic Biosciences (1 mentions)
Cd4 cre mice, by Taconic Biosciences (1 mentions)
Isoflurane, by Fujifilm (1 mentions)
Mrl mpjjmsslc lpr lpr mrl lpr mice, by Japan SLC (1 mentions)
Cd19 cre, by Jackson ImmunoResearch (1 mentions)
Jh mice, by Taconic Biosciences (1 mentions)
Tlr4flox flox, by Jackson ImmunoResearch (1 mentions)
Rosa26creer, by Jackson ImmunoResearch (1 mentions)
B6.129s4 ccr2tm1ifc j, by Jackson ImmunoResearch (1 mentions)
12 protocols using c57bl 6j b6 mice
1
JEV Infection in C57BL/6j Mice
2
Mouse MHV Infection Study Protocol
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Five‐week‐old, MHV‐free, female C57BL/6J (B6) mice were purchased from Japan SLC. Some mice were inoculated intranasally with MHV and kept in cages in a negatively pressured isolator (KIS‐145; Ishihara Corporation, Tokyo, Japan) in the animal room, which was maintained at a temperature of 23°C ± 5°C, humidity of 55% ± 5%, and 12 h illumination (light period: 8:00–20:00; dark period: 20:00–8:00). A plastic mouse breeding cage (220 × 160 × 125 mm; Ishihara Corporation) with a stainless steel wire top was used. Each cage was filled with approximately 1000 mL bedding (ALPHA‐dri; Shepherd Specialty Papers, Watertown, TN, USA). To avoid artificial transmission of MHV, the bedding was not changed during the experiment. Mice were fed γ‐ray–sterilized pellets (CRF‐1; Oriental Yeast Co., Ltd., Tokyo, Japan) and tap water ad libitum from a plastic bottle. The animal protocol was reviewed and approved (P14‐974) by the Institutional Animal Care and Use Committee at the Graduate School of Agricultural and Life Sciences (University of Tokyo, Tokyo, Japan), and was conducted in accordance with the Animal Experiment Implementation Regulations and Animal Experiment Implementation Manual of the University of Tokyo.
3
Generation and Characterization of Murine Transgenic Knockout Models
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C57BL/6J (B6) mice were purchased from Japan SLC. 129S6/SvEv-Stat1tm1Rds (Stat1 KO) mice and CD4Cre+ mice were purchased from Taconic Biosciences (Germantown, NY, USA). Stat3fl/flCD4Cre+ (Stat3 CKO) mice, C129S2-Stat4tm1Gru/J (Stat4 KO) mice, C.129 S (B6) -Stat5aatm1Mam/J (Stat5a KO) mice and C.129S2-Stat6tm1Gru/J (Stat6 KO) mice were purchased from Oriental Yeast Co. (Tokyo, Japan). T cell-specific Egr2 conditional knockout (Egr2fl/flCD4Cre+: Egr2 CKO) mice were produced by crossing Egr2fl/fl mice (provided from Patrick Charnay (INSERM, France)) and CD4Cre+ mice. Egr2/3 DKO mice (Egr2fl/flEgr3fl/flCD4Cre+) were generated by crossing Egr2 CKO mice with Egr3fl/fl mice17 (link). Control mice were littermates of Egr2fl/fl, CD4Cre+ or wild-type (wild type: WT) mice. All mice were housed in a specific pathogen-free environment. For the experiments, mice were at least 6–8 weeks of age. Mice of 24–28 weeks of age were used for analysis of LAG3+ Tregs. All animal experiments were approved by the ethics committee of the University of Tokyo Institutional Animal Care and Use Committee, and all experiments were conducted based on the approved experimental plan according to the guidelines of the University of Tokyo.
4
Anatomical and Optogenetic Studies in B6 Mice
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Male C57BL/6J (B6) mice (Japan SLC, Shizuoka, Japan) were used for all experiments. At the time of the injection experiments (see below), the mice were 6–10 weeks old for anatomical studies and were 5–7 weeks old for optogenetic studies. All experiments were approved by the Committee for Animal Experiments of Kumamoto University and performed in accordance with the Guidelines for Use of Animals in Experiments of Kumamoto University.
5
Inducing Mucosal Injury in Mice
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We obtained C57BL/6J (B6) mice from Japan SLC, Inc.; Villin-Cre transgenic mice, Lgr5-EGFP-Ires-CreERT2 (Lgr5ki) from Jackson Laboratory; Irf2-flox mice were generated as recently described14 (link) For poly(I:C) (polyinosinic:polycytidylic acid) treatment, mice were injected intraperitoneally with PBS or 2 mg/kg body weight of poly(I:C) for 7 consecutive days. To induce mucosal injury in the colon, mice were given 2% w/v DSS (MP Biochemical, Santa Ana, CA) in drinking water for 5–7 days. DSS-treated mice were weighed daily. All mice were maintained in our SPF animal facility and were used for experiments at 3–4 months of age. All experiments using mice were approved by the Institutional Animal Care Committee of Tokyo Medical and Dental University and were performed in accordance with Tokyo Medical and Dental University guidelines.
6
Murine Models of Autoimmunity
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C57BL/6J (B6) mice (7–11 weeks of age) and MRL/MpJJmsSlc-lpr/lpr (MRL/lpr) mice (7 weeks of age) were purchased from Japan SLC, Inc. (Shizuoka, Japan) or CLEA Japan, Inc. (Tokyo, Japan). Foxp3-IRES-GFP-DTR (Foxp3DTR) mice were obtained from the Jackson Laboratory (Bar Harbor, ME, USA) [28 (link)]. Mice were maintained at the Nagoya City University Animal Facility and at the Animal Care Room in Deperament of Immunology at Nagoya City University approved by the Institutional Animal Care and Use Committee of Nagoya City University (approval number: H29M-01). All mice were maintained under specific pathogen-free conditions.
All experimental procedures were performed under anesthesia using isoflurane (Wako Pure Chemical industries, Ltd, Osaka, Japan).
All experimental procedures were performed under anesthesia using isoflurane (Wako Pure Chemical industries, Ltd, Osaka, Japan).
7
Oshima TBEV Infection Model in B6 Mice
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C57BL/6j (B6) mice were purchased from Japan SLC Corporation. Five-week old female B6 mice were subcutaneously inoculated with 104 PFU of TBEV Oshima 5-10 parent (Oshima-pt) and Oshima clones diluted in 200 µl of EMEM containing 2% FCS. Mock-infected mice were inoculated with the supernatant medium of uninfected BHK cells. The mice were weighed daily and observed for clinical signs. The animal experiments were performed in accordance with the Fundamental Guidelines for Proper Conduct of Animal Experiments and Related Activities in Academic Research Institutions under the jurisdiction of the Ministry of Education, Culture, Sports, Science and Technology. The experimental protocols were approved by the Nagasaki University Animal Care and Use Committee (approval number: 091130-2-7/0912080807-7).
8
Genetically Modified Mouse Strains Protocol
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C57BL/6J (B6) mice were obtained from Japan Slc Inc. (Hamamatsu); B6.SJL-ptprca (B6.SJL, Strain #: 4007) mice congenic at the CD45 locus (CD45.1+CD45.2−) and Jh−/− mice (Strain #: 17758) were from Taconic. Il10rafl/fl (Strain #: 028146; Liu et al., 2012 (link)), CAG-EGFP (Strain #: 003291), Cd19-Cre (Strain #: 006785), Tlr4flox/flox (Strain #: 024872), and Rosa26-CreER (Strain #: 008463) mice were from Jackson Laboratories. Il-10 Venus and Il10fl/fl mice were used as previously reported (Atarashi et al., 2011 (link); Roers et al., 2004 (link)). All mice were maintained in our specific pathogen–free animal facility, and all experiments using mice were approved by the Institutional Animal Care Committee of the Tokyo Medical and Dental University.
9
Obese Mouse Models for Metabolic Research
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Male C57BL/6JHamSlc-ob/ob (ob/ob), C57BLKS/J-db/db (db/db), and C57BL/6J (B6) mice were purchased from Japan SLC, Inc. (Hamamatsu, Japan) or CLEA Japan, Inc. (Tokyo, Japan) and were maintained in specific pathogen-free facilities in National institute of infectious diseases. All animals were housed until 18 to 20 wk old with free access to water and diet and provided with 12 h of light/dark cycle. Unexpected death was not observed.
10
Immune Response in IL-19 Knockout Mice
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All animal experiments were conducted under protocols approved by the Animal Experiment Committee of Nagoya University (approved numbers: 15017 and 15018) and Yokohama City University (approved number: F-A-19-036). C57BL/6J (B6) mice were purchased from Japan SLC (Hamamatsu, Japan). IL-19 -/-mice (B6 background) 11, 12 were obtained from Regeneron Pharmaceuticals (Tarrytown, NY, USA).
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