Log In Sign up
The largest database of trusted experimental protocols

T prep method

Manufactured by Covaris
Visit Supplier

The T-prep method is a technique used in sample preparation for various analytical applications. It is designed to facilitate the efficient extraction and purification of target analytes from complex matrices. The T-prep method utilizes a combination of mechanical and chemical processing to ensure the effective liberation and recovery of the desired components. Detailed information about the specific applications and performance characteristics of this method is not available.

Automatically generated - may contain errors

Lab products found in correlation

Quant it ribogreen rna assay, by Thermo Fisher Scientific (2 mentions) Nextseq 500 instrument, by Illumina (2 mentions) Ribo zero rrna removal beads, by Illumina (2 mentions) Truseq stranded total rna kit, by Illumina (2 mentions) Allprep dna rna ffpe kit, by Qiagen (2 mentions) Agilent rna 6000 nano kit, by Agilent Technologies (2 mentions) Agencourt rnaadvance, by Beckman Coulter (2 mentions) Biomek fxp automation, by Beckman Coulter (2 mentions)

2 protocols using t prep method

1

RNA Extraction from Frozen and FFPE Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
A small piece of each snap-frozen primary tumor was pulverized using the T-prep method (Covaris, cat # 520097) on dry ice, followed by implementation of Agencourt RNAadvance (Beckman Coulter, cat # A32645) on a Biomek® FXP automation workstation (Beckman Coulter) to isolate RNA. We used 150 ng RNA to prepare libraries with TruSeq Stranded Total RNA kits (Illumina, cat #RS-122–2301). From FFPE tissues, we deparaffinized four 10-μm sections, rehydrated these in an ethanol/water series, and extracted RNA using AllPrep DNA/RNA FFPE kits (Qiagen). We measured concentrations using the Quant-iT RiboGreen RNA assay (Thermo Fisher) and assessed quality on an Agilent 2100 Bioanalyzer using the Agilent RNA 6000 Nano kit. Ribosomal and mitochondrial RNAs were removed using biotinylated, target-specific oligonucleotides combined with Ribo-Zero rRNA removal beads and the TruSeq Stranded Total RNA kit (Illumina). 75-bp single-end reads were sequenced on a NextSeq 500 Instrument (Illumina).
Cejas P., Drier Y., Dreijerink K.M., Brosens L.A., Deshpande V., Epstein C.B., Conemans E.B., Morsink F.H., Graham M.K., Valk G.D., Vriens M.R., Fernandez-del Castillo C., Ferrone C., Adar T., Bowden M., Whitton H.J., Da Silva A., Font-Tello A., Long H.W., Gaskell E., Shoresh N., Heaphy C.M., Sicinska E., Kulke M.H., Chung D.C., Bernstein B.E, & Shivdasani R.A. (2019). Enhancer signatures stratify and predict outcomes of non-functional pancreatic neuroendocrine tumors. Nature medicine, 25(8), 1260-1265.
+ Open protocol
+ Expand
2

RNA Extraction from Frozen and FFPE Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
A small piece of each snap-frozen primary tumor was pulverized using the T-prep method (Covaris, cat # 520097) on dry ice, followed by implementation of Agencourt RNAadvance (Beckman Coulter, cat # A32645) on a Biomek® FXP automation workstation (Beckman Coulter) to isolate RNA. We used 150 ng RNA to prepare libraries with TruSeq Stranded Total RNA kits (Illumina, cat #RS-122–2301). From FFPE tissues, we deparaffinized four 10-μm sections, rehydrated these in an ethanol/water series, and extracted RNA using AllPrep DNA/RNA FFPE kits (Qiagen). We measured concentrations using the Quant-iT RiboGreen RNA assay (Thermo Fisher) and assessed quality on an Agilent 2100 Bioanalyzer using the Agilent RNA 6000 Nano kit. Ribosomal and mitochondrial RNAs were removed using biotinylated, target-specific oligonucleotides combined with Ribo-Zero rRNA removal beads and the TruSeq Stranded Total RNA kit (Illumina). 75-bp single-end reads were sequenced on a NextSeq 500 Instrument (Illumina).
Cejas P., Drier Y., Dreijerink K.M., Brosens L.A., Deshpande V., Epstein C.B., Conemans E.B., Morsink F.H., Graham M.K., Valk G.D., Vriens M.R., Fernandez-del Castillo C., Ferrone C., Adar T., Bowden M., Whitton H.J., Da Silva A., Font-Tello A., Long H.W., Gaskell E., Shoresh N., Heaphy C.M., Sicinska E., Kulke M.H., Chung D.C., Bernstein B.E, & Shivdasani R.A. (2019). Enhancer signatures stratify and predict outcomes of non-functional pancreatic neuroendocrine tumors. Nature medicine, 25(8), 1260-1265.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!