Mda mb 468

Human cancer cell lines MDA-MB-468, DLD-1, and A431 that express EGFR and negative control (no EGFR expression) MDA-MB-435 cell line were obtained from ATCC (Rockville, MD, USA). Cells were propagated by serial passage in MEM/EBSS medium, supplemented with 10 % fetal bovine serum (Biochrom) at 37°C in a humidified atmosphere of 5 % CO2.
All animals used in imaging experiments were cared for and maintained under the supervision and guidelines of the University of Saskatchewan Animal Care Committee (protocol # 20160005). Female CD-1 nude mice were obtained from Charles River Canada (St-Constant, Quebec) at 4 weeks of age and housed in a 12 h light, 12 h dark cycle in a temperature and humidity controlled vivarium. Animals had libitum access to mouse diet (Lab Diet, St. Louis, Missouri) and water. After one week of acclimatization, mice were subcutaneously injected with a suspension of 5 – 10 × 10⁶ MDA-MB-468, DLD-1, A431 or MDA-MB-435 cells in 100 μL of a 1:1 mixture of serum-free MEM/EBSS medium (HyClone Laboratories, Logan, Utah) and Matrigel matrix basement membrane (Discovery Laboware, Inc. Bedford, MA) at the right? hind limb of each mouse. Tumor growth was followed with caliper measurements.

All human cell lines were purchased from the American Type Culture Collection (ATCC, USA). MCF-7 (luminal A) and MDA-MB-231 (triple-negative breast cancer (TNBC); claudin-low) were cultured in DMEM, whereas MDA-MB-468 (TNBC; basal) were cultured in RPMI 1640 supplemented with 10% (v/v) thermally inactivated fetal bovine serum (Hyclone Laboratories, USA). To generate a 3D model, cells were seeded in ultra-low attachment round-bottom 96-well plates (Corning, USA) and allowed to form multicellular aggregates after 48 h [16 (link), 17 (link)]. All cells were incubated at 37°C and 5% CO₂.