Nci h1299

A cohort of 30 paired NSCLC specimens and adjacent tissues were obtained from Shanxi Provincial Cancer Hospital between December 2019 to December 2020. These specimens were immediately frozen and stored in liquid nitrogen. This study was approved by the Research Ethics Committee of Shanxi Provincial Cancer Hospital and informed consent was obtained from all patients. A copy of the ethical approval was provided in Supplementary material 1. NSCLC cell lines HCC827, NCI-H1299, A549 and NCI-H1650 and the normal lung epithelial cell BEAS-2B were obtained from COBIOER (Nanjing, China) and were cultured in Roswell Park Memorial Institute (RPMI) 1640 basic medium supplemented with 10% fetal bovine serum (FBS) in a humidified with 5% CO₂ at 37°C.

We obtained human lung bronchial epithelial (BEAS-2B, cat# CBP60577) from COBIOER Biosciences CO., LTD (Nanjing, China) and a number of lung cancer cells from the Cell Bank of the Chinese Academy of Science (Shanghai, China), including A549 (cat# SCSP-503), PC-9 (cat# SCSP-5085), HCC827 (cat# SCSP-538), NCI-H460 (cat# SCSP-584), NCI-H1299 (cat# SCSP-589), NCI-H1915 (cat# SCSP-597), and H1650 (cat# SCSP-592). Cells were maintained in the recommended medium, DEME medium for BEAS-2B, or RPMI 1640 for the rest of the cell lines (Procell, China) with the addition of 10% fetal bovine serum (Hyclone, Life Sciences, Shanghai, China), penicillin G (100 U/ml, Beyotime, China), streptomycin (100 μg/ml, Corning, China) in a humidified incubator with 5% CO₂, at 37 °C.