Vybrant mtt cell proliferation assay kit mtt

Chitosan, GTMAC (purity ≥ 90%), TPP (purity ≥ 98%), sodium nitrite (purity ≥ 97%), lipopolysaccharides (LPS) from Salmonella enteric serotype typhimurium (purity ≥ 99%) were purchased from Sigma-Aldrich (St. Louis, USA). Chitosan was purified before its subsequent application, and its molecular weight was tailored by depolymerization using sodium nitrite following a reported method [34 (link)]. Viscosity-average molecular weight of the resultant Chitosan was measured as 1.8×10⁴ Da using a 0.5 M CH₃COOH/0.2 M CH₃COONa solvent system [35 ]. Oligofectamine (OF), 4′,6-diamidino-2-phenyl-indole dihydrochloride (DAPI), FITC fluorescently tagged siRNA (FITC-siRNA) and Vybrant^® MTT cell proliferation assay kit (MTT) were obtained from Invitrogen (Eugene, USA). GelRed was from Biotium (Hayward, USA). siTNF was purchased from Santa Cruz Biotechnology (Santa Cruz, CA), and its sequences were CGUCGUAGCAAACCACCAATT (sense strand) and UUGGUGGUUUGCUACGACGTG (anti-sense strand). All commercial products were used without further purification unless otherwise stated. Deionized water was obtained using a Milli-Q water purification system.

Chitosan, urocanic acid, sodium nitrite, 2-(N-morpholino) ethanesulfonic acid sodium salt (MES), 1-ethyl-3-(diethylaminopropyl)carbodiimide (EDC), N-hydroxysuccinimide (NHS), lipopolysaccharides (LPS) from Salmonella enteric serotype typhimurium were purchased from Sigma-Aldrich (St. Louis, USA). Molecular weight of Chitosan was tailored by depolymerization using sodium nitrite following a reported method [28 (link)]. Viscosity-average molecular weight of the resultant Chitosan was measured as 1.8×10⁴ using a 0.5 M CH₃COOH/0.2 M CH₃COONa solvent system [29 ]. Oligofectamine^™ (OF), 4′,6-diamidino-2-phenyl-indole dihydrochloride (DAPI), FITC fluorescently tagged siRNA (FITC-siRNA) and Vybrant^® MTT cell proliferation assay kit (MTT) were obtained from Invitrogen (Eugene, USA). GelRed was from Biotium (Hayward, USA). siCD98 was purchased from Santa Cruz Biotechnology (Santa Cruz, USA). All commercial products were used without further purification.