Quick start protein assay
The Quick Start protein assay is a colorimetric-based assay used to determine the concentration of protein in a sample. It provides a simple and accurate method for quantifying protein levels in a variety of biological samples.
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10 protocols using quick start protein assay
Preparation of Toxoplasma gondii Antigen
Tyrosine-Linked DNA Substrate Assay
Protein Concentration Measurement by Bradford Method
Example 4
The concentration of a protein was measured by a Bradford method. In the Bradford method, Quick Start Protein Assay (Bio-Rad Laboratories, Inc.) was used, and the protein concentration was calculated based on a standard curve drawn using bovine γ-globulin as a standard protein.
The results of measurement of protein concentration are shown in
Protein Extraction and Immunoblotting
Nrf2 Protein Extraction and Analysis
Quantification of Hepatic p53 Levels
Quantifying Skin Barrier Cytokine Levels
Extracellular ATP Quantification in Na2S-Treated Cells
Remaining cells were suspended in 0.1 N NaOH and sonicated. The protein content of cell lysates was measured using the Quick Start protein assay (Bio-Rad, Hercules, CA). ATP in the samples was measured with the luciferin-luciferase technique using the ATP Determination kit (Invitrogen) and a microplate reader (SH-9000; Corona Electric). A calibration curve was prepared using standard solutions containing known concentrations of ATP, and concentrations of ATP in the samples were calculated from it. The amounts of ATP were expressed as the extracellular amount per milligram of protein in cell lysates (pmol/mg protein).
Quantifying MAPK Phosphorylation in Plant Cells
Cytokine and Chemokine Quantification in Rat
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