5-μm paraffin-embedded tissue sections were deparaffinized in xylene and rehydrated using an ethanol gradient. Tissue sections were incubated with 3% hydrogen peroxide in PBS for 30 minutes at room temperature. Epitope retrieval was performed by treating the tissues with 10 mM sodium citrate buffer (pH 6.0) with 0.05% Tween 20 at 100° C for 10 minutes in a pressure cooker. Ly-6G staining sections were blocked with 10% normal goat serum with 1% BSA in TBS for 2 h at room temperature followed by incubation with rat monoclonal anti-Ly6g antibody (1: 500 dilution) (Abcam, Cambridge, MA, USA. ab25377) in TBS with 1% BSA at 4°C overnight. Tissue sections were treated with their respective biotinylated secondary antibodies for 45 minutes at room temperature (Vector laboratories PK-6101 and BA-9400). Color was developed using the Vectastain ABC kit (Vector Laboratories) followed by DAB reaction. Sections were then counterstained with hematoxylin, dehydrated in an ethanol and xylene. Images were acquired at 20X magnification using an Olympus microscope equipped with a D-26 color camera.
Rat monoclonal anti ly6g antibody
Manufactured by Abcam
Sourced in United States
The Rat monoclonal anti-Ly6g antibody is a laboratory tool used to detect the Ly6g protein in samples. It is a rat-derived monoclonal antibody that specifically binds to the Ly6g antigen.
Automatically generated - may contain errors
Lab products found in correlation
Pk 6101, by Vector Laboratories (1 mentions)
Ba 9400, by Vector Laboratories (1 mentions)
D 26 color camera, by Olympus (1 mentions)
Vectastain abc kit, by Vector Laboratories (1 mentions)
Zinc fixative, by BD (1 mentions)
Rabbit monoclonal anti cleaved caspase 3 antibody, by Cell Signaling Technology (1 mentions)
Anti cd206 antibody, by Abcam (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Confocal microscope, by Olympus (1 mentions)
2 protocols using rat monoclonal anti ly6g antibody
1
Ly-6G Immunohistochemistry in Paraffin Sections
2
Assessing Innate Immunity after Fractional Laser
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To confirm whether innate immunity was induced after the fractional laser treatment, immunohistochemistry was performed. Tumors were excised on day 9 (1 days after aFP) and paraffin sections were made using zinc fixative (BD, Bioscience, Franklin Lakes, NJ) in the same manner as mentioned above. Slides were deparaffinized and washed with PBS or 0.1% triton solution 5 minutes 3 times. Slides were incubated with 10% goat serum in PBS solution for 60 minutes at room temperature (RT) and next a 1:200 dilution of rat monoclonal anti-Ly-6G antibody (Abcam, Cambridge, MA) or anti-CD206 antibody (Abcam), or a 1:100 dilution of rabbit monoclonal anti-cleaved caspase 3 antibody (Cell Signaling Technology, Danvers, MA) in 10% goat serum in PBS was added for overnight incubation at 4°C. A 1:200 dilution of fluorescence-labeled secondary goat anti-rat IgG antibody (Life technology, Carlsbad, CA) in 10% goat serum in PBS was added for 60 minutes at RT on next day. DAPI (Life technology) was used as a nuclear counterstain. To observe fluorescence, a confocal microscope with wave lengths of 405, 561 and 640 nm was used (Olympus, Tokyo, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!