Biotin donkey anti rabbit antibody

Kidneys were harvested from mice and immediately placed in formalin for a minimum of 3 days before embedding in paraffin. For IgG3 staining, de-paraffinised sections were incubated with goat anti-mouse IgG3 antibody (Jackson ImmunoResearch Laboratories, West Grove, PA) for 1 hr, then incubated for 12 min with biotin-donkey anti-goat IgG antibody (Jackson ImmunoResearch Laboratories) and visualized with an iVIEW Plus Detection Kit (Ventana Medical Systems, Tucson, AZ). Staining for IgG1 was performed with a rabbit anti-mouse IgG1 antibody (Novus Biologicals, Littleton, CO), followed by biotin-donkey anti-rabbit antibody (Jackson ImmunoResearch Laboratories). All antibodies were used at 1:100 dilutions and all staining was performed with a Discovery XT (Ventana Medical Systems).