Log In Sign up
The largest database of trusted experimental protocols

Gata4 v5

Manufactured by Promega
Visit Supplier

The )GATA4-V5 is a laboratory product designed for use in various research applications. It is a recombinant protein that contains the GATA4 transcription factor sequence fused with a V5 epitope tag. The core function of this product is to facilitate the study of GATA4 and its interactions within biological systems. Detailed information about the intended use or applications of this product is not available.

Automatically generated - may contain errors

Lab products found in correlation

Pfn21a, by Promega (2 mentions) Gibson assembly master mix, by New England Biolabs (2 mentions) Ptripz inducible lentiviral vector, by Thermo Fisher Scientific (2 mentions) Quick ligation kit, by New England Biolabs (2 mentions)

2 protocols using gata4 v5

1

Generation of Inducible FOXA2, RFP, and FOXA2-CDT1 Clones

Check if the same lab product or an alternative is used in the 5 most similar protocols
To generate pTRIPZ-FOXA2, RFP, FOXA2-CDT1 clones, pTRIPZ inducible lentiviral vector (Thermo Scientific) and full-length FOXA2 were assembled using Gibson Assembly® Master Mix (NEB). pTRIPZ empty vector was digested with XhoI and MluI to remove shRNAmir regulatory sequences, and digested ends were blunted. The linearized pTRIPZ backbone was digested with BsiWI to generate two fragments, each with one sticky end. The fragments were gel extracted, purified, and ligated using the Quick LigationTM Kit (NEB). Primer sequences are listed in Supplementary Table 1.
To generate HaloTagged-FOXA2 construct, full-length FOXA2 was ligated to pFN21A (Promega)
GATA4-V5 and POU5F1-V5 constructs were obtained from the Broad Institutes Genomics Perturbations platform and are available to purchase through Thermo Fisher.
Donaghey J., Thakurela S., Charlton J., Chen J., Smith Z.D., Gu H., Pop R., Clement K., Stamenova E., Karnik R., Kelley D.R., Gifford C.A., Cacchiarelli D., Rinn J.L., Gnirke A., Ziller M.J, & Meissner A. (2018). Genetic determinants and epigenetic effects of pioneer factor occupancy. Nature genetics, 50(2), 250-258.
+ Open protocol
+ Expand
2

Generation of Inducible FOXA2, RFP, and FOXA2-CDT1 Clones

Check if the same lab product or an alternative is used in the 5 most similar protocols
To generate pTRIPZ-FOXA2, RFP, FOXA2-CDT1 clones, pTRIPZ inducible lentiviral vector (Thermo Scientific) and full-length FOXA2 were assembled using Gibson Assembly® Master Mix (NEB). pTRIPZ empty vector was digested with XhoI and MluI to remove shRNAmir regulatory sequences, and digested ends were blunted. The linearized pTRIPZ backbone was digested with BsiWI to generate two fragments, each with one sticky end. The fragments were gel extracted, purified, and ligated using the Quick LigationTM Kit (NEB). Primer sequences are listed in Supplementary Table 1.
To generate HaloTagged-FOXA2 construct, full-length FOXA2 was ligated to pFN21A (Promega)
GATA4-V5 and POU5F1-V5 constructs were obtained from the Broad Institutes Genomics Perturbations platform and are available to purchase through Thermo Fisher.
Donaghey J., Thakurela S., Charlton J., Chen J., Smith Z.D., Gu H., Pop R., Clement K., Stamenova E., Karnik R., Kelley D.R., Gifford C.A., Cacchiarelli D., Rinn J.L., Gnirke A., Ziller M.J, & Meissner A. (2018). Genetic determinants and epigenetic effects of pioneer factor occupancy. Nature genetics, 50(2), 250-258.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!