Antibody staining was performed in PBS containing 2% BSA or FBS (wt/vol). For analysis of cytokine production, splenocytes were first stimulated with GP33 peptide (amino acid sequence: KAVYNFATC, 0.2 μg/ml) in the presence of brefeldin A for 5 hrs at 37 °C. Following surface staining, intracellular cytokine staining was performed with a Cytofix/Cytoperm Fixation/Permeabilization kit (554714, BD Biosciences) according to the manufacturer’s instructions. To detect degranulation, splenocytes were stimulated for 5 h with the indicated peptide (0.2 μg/ml), in the presence of brefeldin A and anti-CD107a/b antibodies (1D4B, BD Biosciences). Major histocompatibility complex (MHC) class I peptide tetramers consisting of H-2Db complexed with LCMV GP33-41 were obtained from Dr. Rafi Ahmed (Emory University). Samples were collected using a FACSCanto system (BD Bioscience) and analyzed with FlowJo software (TreeStar).
Anti cd107a b antibodies 1d4b
Manufactured by BD
Anti-CD107a/b antibodies (1D4B) are laboratory reagents used to detect and analyze the activation of cytotoxic lymphocytes, such as natural killer (NK) cells and cytotoxic T cells. These antibodies specifically bind to the CD107a and CD107b proteins expressed on the surface of activated cytotoxic cells, allowing for their identification and quantification.
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Lab products found in correlation
2 protocols using anti cd107a b antibodies 1d4b
1
LCMV-specific CD8+ T Cell Characterization
2
LCMV-specific CD8+ T Cell Characterization
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Antibody staining was performed in PBS containing 2% BSA or FBS (wt/vol). For analysis of cytokine production, splenocytes were first stimulated with GP33 peptide (amino acid sequence: KAVYNFATC, 0.2 μg/ml) in the presence of brefeldin A for 5 hrs at 37 °C. Following surface staining, intracellular cytokine staining was performed with a Cytofix/Cytoperm Fixation/Permeabilization kit (554714, BD Biosciences) according to the manufacturer’s instructions. To detect degranulation, splenocytes were stimulated for 5 h with the indicated peptide (0.2 μg/ml), in the presence of brefeldin A and anti-CD107a/b antibodies (1D4B, BD Biosciences). Major histocompatibility complex (MHC) class I peptide tetramers consisting of H-2Db complexed with LCMV GP33-41 were obtained from Dr. Rafi Ahmed (Emory University). Samples were collected using a FACSCanto system (BD Bioscience) and analyzed with FlowJo software (TreeStar).
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