All experimental procedures were conducted in strict adherence to the European Union Directive of 22 September 2010 (2010/63/UE) and approved by the French Ministry of Research. A total of 318 Swiss male mice (Janvier, Saint Berthevin, France) were used in this study, and only three deaths were reported. During the experimental period, up to six mice were housed per cage in a controlled environment (temperature: 22 ± 2°C, humidity: 50 ± 10%, 12 h/12 h light-dark cycle with lights off at 7 pm) with free access to food and water. The group sizes used in this study were advised by the contract research organization which conducted the experiments (Amylgen) which has more than 12 years of expertise in the Aβ25–35 mouse model.
Male swiss mice
Manufactured by Janvier Labs
Sourced in France
Male Swiss mice are a commonly used laboratory animal model. They are rodents of the species Mus musculus, specifically the Swiss Webster strain. These mice are known for their gentle temperament and are widely used in a variety of research applications.
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11 protocols using male swiss mice
1
Aβ25-35 Mouse Model Protocol
2
Behavioral and Histological Effects of Oxaliplatin-Induced Neuropathy in Mice
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Swiss male mice (6–7 weeks old, 25–30 g) from Janvier labs (France) were housed in plastic cages and maintained on a 12 h light/dark cycle with food and water available ad libitum (BISCEm-animal care and facility center). Mice were randomly assigned to OXP or its vehicle (control group: CTRL), thereafter mice were randomly assigned to ramipril treatment or its vehicle, defining the following four treatment groups: control-vehicle (CTRL-VEH), oxaliplatin-vehicle (OXP-VEH), control-ramipril (CTRL-RAM) and oxaliplatin-ramipril (OXP-RAM).
Behavioral tests were performed on day 1 (D1), D3, and D5 following the last OXP injection, except for the jump test, which was performed on D1 and D3 only to avoid acclimation of the mice to the test. All animals were submitted to the functional tests, except for the jump test, the day before the start of experiment (reference day: RD) to obtain the baseline for each animal. Immunohistochemistry and morphological analyses were performed one day after the last OXP injection, the time corresponding to the maximum of sensory impairment (Figure 1 ).
Behavioral tests were performed on day 1 (D1), D3, and D5 following the last OXP injection, except for the jump test, which was performed on D1 and D3 only to avoid acclimation of the mice to the test. All animals were submitted to the functional tests, except for the jump test, the day before the start of experiment (reference day: RD) to obtain the baseline for each animal. Immunohistochemistry and morphological analyses were performed one day after the last OXP injection, the time corresponding to the maximum of sensory impairment (
3
Swiss Male Mice Experiments
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Experiments were performed on swiss male mice (35-40 g; Janvier Labs, France, 8-10 weeks old) in accordance with French ethical laws (Decree 2013-118) and European Communities Council guidelines (2010/63/EU).
4
Mouse Housing and Feeding Protocols
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Male Swiss mice (Janvier Laboratories, Saint Isle, France). Mice were fed ad libitum.
Female BALB/c mice aged 5 to 7 weeks were purchased from Janvier Laboratories, France. Mice were housed at 5 animals per cage with sterile bedding in HEPA–filtered racks (Tecniplast, France) in certified animal biosafety level 3 (ABSL-3) laboratories. Temperatures were maintained at 65-75°F (∼18-23 °C) with 40-60% humidity. Specific pathogen-free status was verified by testing sentinel mice housed within the same rack. Mice had ad libitum access to water and food pellets.
For combination studies, Female BALB/c mice 6-8 weeks old, purchased from Charles River (Wilmington, MA, USA) were used.
5
Mouse Husbandry and Euthanasia
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Male and female FVB (20–25 g) and male Swiss mice (20–25 g) were purchased from Janvier (Genest, France). Animals were housed in well-ventilated cages at 20–22°C with 45–65% relative humidity and maintained under a 12-hour dark/light cycle (light from 8:00 a.m. to 8:00 p.m.) for at least one week before the experiments. Food and water were provided ad libitum. Following each experiment, mice were euthanized using a carbon dioxide chamber. All animals were treated in accordance with the ethical guidelines established by the National Institutes of Health and the French Ministry of Agriculture. Protocols followed the animal facility experimental procedures of the Paris-Descartes University were approved by the institutional ethics committee.
6
Swiss Mouse Housing and Handling
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Male Swiss mice (Janvier, France) weighing 23 to 25g at the beginning of the experiments were housed 5/cage on a 12-hour-light/-dark cycle in a temperature-controlled room (21±2°C) with food and water available ad libitum. Animal experiments were carried out in accordance with the European Communities Council Directive of 24 November 1986 (86/609/EEC) as well as French law, with the standard ethical guidelines, and under the control of the Ethical Committee of the faculty (N° CEEA34.NM.117.12). Every effort was made to reduce the number of animals used and their discomfort.
7
Mouse Housing and Behavioral Experiments
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Male swiss mice, 6 weeks old, weighing 32 ±
2 g, from JANVIER labs (Saint Berthevin, France) were kept in for
housing, and experiments took place within the animal facility building
of the University of Montpellier (CECEMA, Office of Veterinary Services
agreement # B-34-172-23). Upon arrival, animals were divided into
groups and housed with access to food and water ad libitum, except
during behavioral experiments. Mice were kept in a temperature- and
humidity-controlled animal facility on a 12 h/12 h light/dark cycle
(lights off at 07:00 PM). Behavioral experiments were conducted between
9:00 and 17:00 in a sound-attenuated and air-regulated experimental
room. All animal procedures were conducted in strict adherence to
the European Union directive of September 22, 2010 (2010/63/UE) and
the ARRIVE guidelines. The project was authorized by the French National
Ethic Committee (APAFIS no. 148515034).85 (link)
2 g, from JANVIER labs (Saint Berthevin, France) were kept in for
housing, and experiments took place within the animal facility building
of the University of Montpellier (CECEMA, Office of Veterinary Services
agreement # B-34-172-23). Upon arrival, animals were divided into
groups and housed with access to food and water ad libitum, except
during behavioral experiments. Mice were kept in a temperature- and
humidity-controlled animal facility on a 12 h/12 h light/dark cycle
(lights off at 07:00 PM). Behavioral experiments were conducted between
9:00 and 17:00 in a sound-attenuated and air-regulated experimental
room. All animal procedures were conducted in strict adherence to
the European Union directive of September 22, 2010 (2010/63/UE) and
the ARRIVE guidelines. The project was authorized by the French National
Ethic Committee (APAFIS no. 148515034).85 (link)
8
Animal Housing and Ethical Approval Protocol
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Animals were housed in groups under a 12:12 h light/dark cycle (lights on at 7:00 a.m.) at a controlled temperature of 21 ± 2℃ and humidity of 45 ± 15% with free access to food and water, except when noted below. Experiments were conducted in accordance with European ethical standards (2013/118/EEC) and approved by the local ethical committee (CEA n° 79). Male Wistar rats (220–300 g) and male Swiss mice were obtained from Janvier (Le Genest Saint‐Isle, France). Male C57BL/6J and male OF1 mice were obtained from Charles River (Saint‐Germain‐Nuelles, France).
9
Male Swiss Mice Housing and Experiments
10
Swiss Male Mice Acclimation Protocol
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All experimental procedures were approved by the SSA animal ethics committee according to applicable French legislation (Directive 2010/63/UE, decret 2013-118). To avoid any potential sexually dimorphic effect, only male Swiss mice (Janvier Labs, Genest-Saint-Isle, France) aged 7 to 8 weeks were housed, with four per cage on a 12 h/12 h light/dark cycle with food and water ad libitum. After a 7-day acclimation period, the animals were randomly assigned to their dedicated experiment. Animal group housing was the same before and after the NIMP challenge. Nine-week-old animals were used for the OP challenge.
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