Dako envision system kit

Manufactured by Agilent Technologies

Sourced in United States

The DAKO Envision System kit is a labeled polymer detection system used in immunohistochemistry (IHC) and in situ hybridization (ISH) procedures. It is designed to detect primary antibodies or labeled oligonucleotide probes in tissue sections or cell preparations.

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Lab products found in correlation

Balb c nude mice, by Jackson ImmunoResearch (2 mentions) Anti nf κb sc 71677, by Santa Cruz Biotechnology (2 mentions) Anti nkrf hpa001476, by Merck Group (2 mentions) Ab6672, by Abcam (1 mentions) Formalin, by Merck Group (1 mentions) Af 401 na, by R&D Systems (1 mentions) Vectastain universal quick kit, by Vector Laboratories (1 mentions) Imagej, by Media Cybernetics (1 mentions) Sc 133192, by Santa Cruz Biotechnology (1 mentions) Digital caliper, by Thermo Fisher Scientific (1 mentions) Sz51 stereomicroscope, by Olympus (1 mentions) Dfc300fx camera, by Leica (1 mentions) Nanozoomer slide scanner, by Hamamatsu Photonics (1 mentions) Ndp view2, by Hamamatsu Photonics (1 mentions)

Close spelling variants of this product

DAKO Envision+ system peroxidase kit Dako REAL EnVision Detection System Peroxidase/DAB+kit Dako EnVision® Dual Link System-HRP (DAB+) kit Dako REAL EnVision Detection System kit DAKO EnVision Detection System kit DAKO EnVision+ System-HRP kit Dako EnVision+System HRP labelled polymer detection kit Dako EnVision + system-HRP (DAB) kit Dako EnVision + System- HRP Labelled Polymer kit Dako Envision system

6 protocols using dako envision system kit

Immunohistochemical Analysis of Antioxidant Proteins

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Nude BALB/C mice were purchased from Jackson laboratories (Bar Harbor, ME, USA). DAKO EnVision+ system kits (K4007, K4011) were purchased from Agilent technologies (Carpinteria, CA, USA). All animal studies were conducted in accordance with National Institutes of Health animal use guidelines, and a protocol approved by the University of Louisville’s Institutional Animal Care and Use Committee (IACUC).
Rabbit polyclonal anti-MnSOD (06-984) was purchased from Upstate antibodies (Millipore, Temecula, CA, USA). Mouse monoclonal anti-NF-κB (sc-71677) was purchased from Santa Cruz biotechnology (Dallas, TX, USA). Rabbit polyclonal anti-Nkrf (HPA001476) was purchased from Sigma Aldrich (St. Louis, MO, USA).

Pandit H., Zhang W., Li Y., Agle S., Li X., Li S.P., Cui G., Li Y, & Martin R.C. (2015). Manganese Superoxide Dismutase (MnSOD) expression is negatively associated with microRNA-301a in human Pancreatic Ductal Adenocarcinoma (PDAC). Cancer gene therapy, 22(10), 481-486.

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Immunohistochemical Analysis of Antioxidant Proteins

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Neuropathological Assessment of Tissue Samples

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Formalin‐fixed, paraffin‐embedded tissue blocks from the tumor region, distant regions with areas sensitive to neurodegeneration (frontal cortex, basal ganglia, hippocampus, amygdala, pons, medulla oblongata; Figure 1) were stained with hematoxylin and eosin (H&E; Figure 3A) and by immunohistochemistry applying a panel of primary antibodies (Table 1). Immunoreaction was visualized by the DAKO Envision System kit (DAKO, Glostrup, Denmark) using the Dako‐Autostainer 48 Link platform, diaminobenzidine was used as chromogen.

Klotz S., Ricken G., Preusser M., Dieckmann K., Widhalm G., Rössler K., Fischer P., Kalev O., Wöhrer A., Kovacs G.G, & Gelpi E. (2022). Enhanced expression of autophagy‐related p62 without increased deposits of neurodegeneration‐associated proteins in glioblastoma and surrounding tissue – An autopsy‐based study. Brain Pathology, 32(5), e13058.

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Quantitative Analysis of Inflammatory Cytokines in Maxillary Tissue

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The maxillary tissues were fixed with 10% formalin (Sigma Aldrich, USA), decalcified with 0.5 M ethylene-diamine tetra acetic acid (EDTA, pH 7.4), and paraffin-embedded. The specimens were sliced into 5 µm thick sections, and TRAP and hematoxylin-and-eosin (H&E) staining analyses were performed, as previously mentioned [2 (link)]. TRAP-positive cells were counted using Image J (Media Cybernetics, Inc. Rockville, MD, USA) [33 (link)].
To identify proinflammatory cytokine expressions, IHC analysis was performed with primary antibodies against IL-1β (AF-401-NA, R&D Systems, Minneapolis, MN, USA), IL-6 (ab6672, Abcam, UK), and TNF-a (sc-133192, Santa Cruz, CA, USA). For the secondary antibody reactions, VECTASTAIN^® Universal Quick Kit (PK-7800, Vector Laboratories, Burlingame, CA, USA) for IL-1β and Dako EnVision+ System Kit (K4009, Dako, Denmark) for IL-6 and TNF-a were used. The DAB Peroxidase Substrate Kit was used to visualize the cytokine expressions in the tissues [2 (link)]. The quantitative comparisons were conducted by calculating the percentage of positive staining on the same-sized area in the furcation of the maxillary second molar using Image J Fiji (Version 1.2).

Zhang Z., Song J., Kwon S.H., Wang Z., Park S.G., Piao X., Ryu J.H., Kim N., Kim O.S., Kim S.H, & Koh J.T. (2023). Pirfenidone Inhibits Alveolar Bone Loss in Ligature-Induced Periodontitis by Suppressing the NF-κB Signaling Pathway in Mice. International Journal of Molecular Sciences, 24(10), 8682.

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Intestinal Polyp Visualization and Characterization

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Methylene blue‐stained polyps were visualized under an SZ51 stereomicroscope (Olympus, Richmond Hill, Canada). Polyp sizes were measured with a digital caliper (Thermo Fisher Scientific, Waltham, MA) and polyp numbers were counted from the duodenum to the rectum as previously described (Perreault, Sackett, Katz, Furth, & Kaestner, 2005). Tissues were fixed, paraffin embedded, sectioned, and stained as described before (Leblanc et al., 2017). Immunohistochemistry staining was performed with the Dako EnVision+System Kit (Dako, Santa Clara, CA) according to the manufacturer’s recommendations. Immunofluorescence against chromogranin A was performed as described previously (C. S. Lee, Perreault, Brestelli, & Kaestner, 2002). Mucus secretion was visualized by Alcian blue staining performed on distal colon tissues fixed with Carnoy’s solution (10% glacial acetic acid, 30% chloroform, and 60% ethanol). For immunofluorescence, images were taken with a Leica DLMB2 microscope equipped with a DFC300FX camera and Leica FireCAM 3.4.1 Software (Leica, Concord, Canada). Otherwise, slides were visualized with a NanoZoomer slide scanner and NDP.view2 software (Hamamatsu, Boston, MA). All cell counts were performed on well‐oriented crypts in a double‐blind manner.

Beaudry K., Langlois M., Montagne A., Cagnol S., Carrier J.C, & Rivard N. (2018). Dual‐specificity phosphatase 6 deletion protects the colonic epithelium against inflammation and promotes both proliferation and tumorigenesis. Journal of Cellular Physiology, 234(5), 6731-6745.

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Immunohistochemical Staining of Tissue Arrays

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Immunohistochemical staining was obtained on the paraffin-embedded blocks of tissue arrays using the DAKO EnVision+ System Kit (DAKO, Carpinteria, California). In brief, the sections were deparaffinized and hydrated. The slides were washed with a Tris buffer, and peroxidase blocking was performed for 5 minutes. After rewashing, the monoclonal antibodies, CD44 and EpCAM, and polyclonal antibodies, CD45, CD90, and CD133, were applied for 60 minutes at room temperature. The slides were rinsed and incubated with labeled polymer for 30 minutes at room temperature. The substrate-chromogen solution (diaminobenzidine) was added as a visualization reagent. As a final step, the slides were counterstained with hematoxylin. A negative control, using the same experimental procedure as the tested slides, except for the primary antibody, was included in each run.

Lin Z., Lu X., Li W., Sun M., Peng M., Yang H., Chen L., Zhang C., Cai L, & Li Y. (2015). Association of Cancer Stem Cell Markers With Aggressive Tumor Features in Papillary Thyroid Carcinoma. Cancer control : journal of the Moffitt Cancer Center, 22(4).

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