The largest database of trusted experimental protocols

C3fe swv mbpshi j

Manufactured by Jackson ImmunoResearch
Sourced in United States

C3Fe.SWV-Mbpshi/J is a strain of genetically modified mouse. It is a congenic strain that was developed by backcrossing the Mbpshi mutation, which affects myelin basic protein, onto the C3H/HeJ genetic background.

Automatically generated - may contain errors

5 protocols using c3fe swv mbpshi j

1

Isolation and Expansion of Mouse Embryonic and Tail Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse embryonic fibroblasts (MEFs) were isolated at embryonic day 13.5 (E13.5) from embryos generated through timed natural matings between shiverer homozygous mice (C3Fe.SWV-Mbpshi/J; Jackson Laboratory). Cells were expanded for one passage and cryopreserved for future use. Wild-type tail tip fibroblasts (TTFs) were generated from postnatal day 9 mice (B6CBACa-Aw-J; Jackson Laboratory). Tail tips of approximately 2 mm in length were bisected with a sterile scalpel and cultured on a Nunclon-Δ wells under glass coverslips for outgrowth. MEFs and TTFs were isolated and expanded in fibroblast medium, which consisted of DMEM supplemented with 10% fetal bovine serum (FBS; Thermo Fisher), 2 mM glutamax (Thermo Fisher), 1× nonessential amino acids (Thermo Fisher), and 0.1 mM 2-mercaptoethanol (Sigma).
+ Open protocol
+ Expand
2

Demyelination and Remyelination in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
All animal experiments were performed in an Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC)-accredited vivarium at Biogen according to the Institutional Animal Care and Use Committee (IACUC) approved protocols. Six to 8 weeks old, male C3HeB/FeJ-shiverer (also known as C3Fe.SWV-Mbpshi/J; referred here as “Shi”) mice and age-matched wild type (WT) controls (C3HeB/FeJ) were obtained from The Jackson Laboratory. To induce demyelination, 8 weeks old, male C57BL/six mice (obtained from The Jackson Laboratory) were fed with standard chow diet (CD) mixed with 0.2% Cz (Research Diets, Inc, NJ) for 6 weeks. Age-matched control mice were fed with CD. After 6 weeks, Cz was removed from the diet and replaced with CD. The study was continued for additional 6 weeks to allow remyelination. Cz- and CD-fed mice were sacrificed at 6- and 12-week study time points.
+ Open protocol
+ Expand
3

Sprague Dawley Rat-Derived Cell Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Young adult Sprague Dawley rats (200–250 g, Charles River Laboratories, Wilmington, MA, USA) were used for bone marrow extraction. OPs were harvested from neonatal Sprague Dawley rats for comparison of marker expression with aMSC-derived OPs. Dorsal root ganglia were harvested from embryonic day 15 Sprague Dawley rats. Homozygous shiverer mice (C3Fe.SWV-MBPShi/J, The Jackson Laboratory, Bar Harbor, ME, USA) were used as recipient of aMSC-derived OPs to demonstrate in vivo myelination as a result of OP transplantation into the corpus callosum. C57BI/6J mice (The Jackson Laboratory) were used to illustrate degree of myelination in the wild type. All experimental protocols were approved by the Committee for Use of Live Animals in Teaching and Research, The University of Hong Kong.
+ Open protocol
+ Expand
4

Transplantation of Zfp488-ZsGreen1/PDGFR-α+ OPCs in Shiverer Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Shiverer mice (C3Fe.SWV-Mbpshi/J, The Jackson Laboratory, USA) lack myelin basic protein and myelin in the CNS39 (link),40 (link). Shiverer mice 8–10 weeks old (N = 3, each group) were anesthetized and injected with 50,000 iPSC derived Zfp488-ZsGreen1/PDGFR-α+ OPCs or sham injected with the same volume of phosphate buffered saline into the corpus callosum (surgical procedure and stereotaxic coordinates were identical with the LPC injections coordinates). Mice were administered cyclosporine 10 mg/kg, intraperitoneally daily for 8 weeks for immune suppression after which they were sacrificed. Brains were processed for electron microscope imaging. One-micron meter sections of the injected areas were analyzed for the formation of the multilayered compact myelin sheath. We could not derive enough control OPCs (with the empty virus) for transplantation using this protocol. All animal procedures were approved by the IACUC committee of UC Davis. All experiments (in vitro and invivo including supplementary information) were performed in accordance with relevant guidelines and regulations.
+ Open protocol
+ Expand
5

Shiverer Mice as a Model for Evaluating OPC Transplantation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Shiverer mice (C3Fe.SWV-Mbpshi/J, The Jackson Laboratory, USA) lack MBP and compact myelin in the CNS. Therefore, these mice serve as a good model to test the functional capacity of transplanted OPCs toward forming MBP-positive compact myelin sheath around axons. Shiverer mice 8–10 weeks old (N = 3; 2 males, 1 female) were injected with 50,000 iPSC-derived GANT61-OLIG2+/GFP+/PDGFR-α+ OPCs. Mice were deeply anesthetized by a cocktail of ketamine (70 mg/kg) and xylazine (10 mg/kg). Then, 1 μL of cell suspension was injected aseptically (at the rate of 0.3 μL/min) into the corpus callosum using a Hamilton syringe at the following stereotaxic coordinates: anteroposterior 0.2, mediolateral 1.1 with Bregma as a reference, and dorsoventral 2 mm from the skull surface. Mice were administered cyclosporin 10 mg/kg daily intraperitoneally for immune suppression, beginning 2 days before cell transplantation until sacrifice. Postoperative care was given according to the Institutional Animal Care and Use Committee (IACUC) protocols approved by the University of California, Davis. Mice were sacrificed after 4 or 6 weeks, and the corpus callosum sections (2–3 mm) containing the injection site was microdissected and processed for imaging under an electron microscope or for confocal imaging.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!