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Lunar piximus2 mouse densitometer

Manufactured by GE Healthcare
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The Lunar PIXImus2 is a mouse densitometer designed to measure bone mineral density (BMD) and body composition in small laboratory animals. It uses dual-energy X-ray absorptiometry (DXA) technology to provide accurate and reliable measurements of bone, fat, and lean tissue.

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Lab products found in correlation

Minispec body composition analyzer, by Bruker (1 mentions) μct40, by Scanco (1 mentions)

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PIXImus2 (13 citations)

7 protocols using lunar piximus2 mouse densitometer

1

Quantifying Adiposity in Mice

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The total body fat content of mice was determined by dual energy X-ray absorptiometry (Lunar PIXImus2 mouse densitometer; GE Healthcare, Chicago, IL, USA), in accordance with the manufacturer’s instructions. Body weight was determined and the left and right epididymal and inguinal white fat pads were harvested and weighed. Epididymal fat pads were then fixed in 10% neutral buffered formalin and embedded in paraffin. Tissues were cut into 4-μm sections and stained with hematoxylin and eosin. For the determination of adipocyte size, photomicrographs of the stained sections were obtained at ×100 magnification. Mean adipocyte diameter was calculated from measurements of at least 200 cells per sample.
Foretz M., Even P.C, & Viollet B. (2018). AMPK Activation Reduces Hepatic Lipid Content by Increasing Fat Oxidation In Vivo. International Journal of Molecular Sciences, 19(9), 2826.
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2

Whole-Body Composition Analysis by DEXA

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Upon completion of indirect calorimetry and physical activity measurements, animals were anesthetized with isoflurane and then scanned for whole-body lean and fat mass using Dual-energy X-ray absorptiometry (DEXA, Lunar PIXImus2 mouse densitometer; GE Healthcare, Waukesha, WI, USA). The head and the tail were excluded from DEXA analyses.
Zhang L., Lee I.C., Enriquez R.F., Lau J., Vähätalo L.H., Baldock P.A., Savontaus E, & Herzog H. (2014). Stress- and diet-induced fat gain is controlled by NPY in catecholaminergic neurons. Molecular Metabolism, 3(5), 581-591.
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3

Body Composition and Skeletal Analysis

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Fat mass was quantified using echoMRI-based MiniSpec Body Composition Analyzer from Bruker. Hindlimb bone mineral density was quantified using Dual-Energy X-ray Absorptiometry (DEXA) (Lunar PIXImus 2 Mouse Densitometer equipment, GE Medical Systems). The same equipment was also used to obtain images for the quantification of thoracic-lumbar curvature. Thymi were weighted after fixation in 10% histological formalin (~48 h). Spleens were fixed similarly and weighted after transfer into 70% EtOH.
Purhonen J., Banerjee R., Wanne V., Sipari N., Mörgelin M., Fellman V, & Kallijärvi J. (2023). Mitochondrial complex III deficiency drives c-MYC overexpression and illicit cell cycle entry leading to senescence and segmental progeria. Nature Communications, 14, 2356.
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4

Body Composition Analysis in Mice

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All mice from both Cartcre/cre and Cartcre/+ groups were subjected to body composition analysis using the dual-energy X-ray absorptiometry (DXA, Lunar PIXImus2 mouse densitometer; GE Healthcare, Waukesha, WI, USA) system at 16–17 weeks of age, immediately following completion of the indirect calorimetry assessment of energy metabolism and physical activity. Animals were anesthetized with isoflurane for the scanning procedure to determine whole body bone mineral density (BMD), bone mineral content (BMC), fat and lean mass, where lean mass corresponds to non-fat and non-bone tissue content. The head of the animal was excluded while the tail included for the analysis.
Lau J., Farzi A., Qi Y., Heilbronn R., Mietzsch M., Shi Y.C, & Herzog H. (2017). CART neurons in the arcuate nucleus and lateral hypothalamic area exert differential controls on energy homeostasis. Molecular Metabolism, 7, 102-118.
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5

Longitudinal Body Composition Analysis

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Body composition was assessed at ~4 week intervals beginning when the pups were weaned at 4 weeks of age. Body composition was measured by dual energy x-ray absorptiometry (DEXA) with a Lunar PIXImus II mouse densitometer (GE Medical Systems, Waukesha WI) with anesthesia by isofluorane inhalation.
Schulfer A.F., Battaglia T., Alvarez Y., Bijnens L., Ruiz V.E., Ho M., Robinson S., Ward T., Cox L.M., Rogers A.B., Knights D., Sartor R.B, & Blaser M.J. (2017). Intergenerational transfer of antibiotic-perturbed microbiota enhances colitis in susceptible mice. Nature microbiology, 3(2), 234-242.
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6

Bone Density and Architecture Analysis

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Ten to twelve mice from each experimental group (GILZ Tg, TNF Tg, and TNF-GILZ Tg) were assessed for bone density and architecture. The left femur of each mouse was dissected free of soft tissues and scanned by DXA densitometry (Lunar PIXImus II mouse densitometer; GE Medical Systems) for bone mineral density (BMD) and bone mineral content (BMC) measurement. Bone structural parameters of the right femur and tibia of each mouse were analyzed by μ-CT scan (μ-CT-40; Scanco Medical AG) as previously described [37 (link)].
Yang N., Baban B., Isales C.M, & Shi X.M. (2017). Role of glucocorticoid-induced leucine zipper (GILZ) in inflammatory bone loss. PLoS ONE, 12(8), e0181133.
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7

Longitudinal Body Composition Analysis

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Body composition was assessed at ~4 week intervals beginning when the pups were weaned at 4 weeks of age. Body composition was measured by dual energy x-ray absorptiometry (DEXA) with a Lunar PIXImus II mouse densitometer (GE Medical Systems, Waukesha WI) with anesthesia by isofluorane inhalation.
Schulfer A.F., Battaglia T., Alvarez Y., Bijnens L., Ruiz V.E., Ho M., Robinson S., Ward T., Cox L.M., Rogers A.B., Knights D., Sartor R.B, & Blaser M.J. (2017). Intergenerational transfer of antibiotic-perturbed microbiota enhances colitis in susceptible mice. Nature microbiology, 3(2), 234-242.
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