Acetonitrile (ACN) and methanol (MeOH) were HPLC grade and purchased from Fisher Scientific (Pittsburgh, PA). Ultrapure water was prepared on a MK-459 Millipore Milli-Q Plus ultrapure water system (Darmstadt, Germany). Ephedrine hydrochloride (purity up to 100%), pseudoephedrine hydrochloride (purity up to 99.8%), and methylephedrine hydrochloride (purity up to 99.6%) were kindly provided by the national institutes for food and drug control. Hydroxychloroquine (HCQ, purity of 98%) was provided by Energy Chemical (Shanghai, China). Ephedra sinica was obtained from the traditional Chinese medicine store (Xi’an, China). The receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 (SARS-CoV-2 RBD, purity >95% as determined by SDS-PAGE) and human ACE2 protein (purity >95% as determined by SDS-PAGE) were supplied by Sino Biological Inc. (Beijing, China). All reagents were used without further purification.
Mk 459 millipore milli q plus ultrapure water system
Manufactured by Merck Group
Sourced in Germany, China
The MK-459 Millipore Milli-Q Plus ultrapure water system is a laboratory equipment product designed to produce high-quality ultrapure water. It is capable of removing contaminants from source water, resulting in water that meets the stringent purity requirements for various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Sars cov 2 rbd, by Sino Biological (1 mentions)
Acetonitrile acn, by Thermo Fisher Scientific (1 mentions)
Hplc grade methanol, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Merck Group (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium dmem, by Merck Group (1 mentions)
Urea, by Merck Group (1 mentions)
Trifluoroacetic acid tfa, by Tedia (1 mentions)
N octyl β d glucopyranoside og, by Merck Group (1 mentions)
Acetonitrile acn, by Tedia (1 mentions)
3 protocols using mk 459 millipore milli q plus ultrapure water system
1
SARS-CoV-2 RBD Binding Assay Protocol
2
Analytical Protocol for Phytochemicals
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
12-Epinapelline, 3-deoxyaconitine, benzoylaconitine and napellonine were from Nanjing SenBeiJia Biological Technology (Jiangsu, China). Nitrendipine, valsartan, captopril, gefitinib and ciprofloxacin were purchased from Nanjing Ange Pharmaceutical Co., Ltd. (Jiangsu, China). PD173074 was from Lanmu Chemical Technology (Shanghai, China). Ammonium acetate and acetic acid were obtained from Fuchen Chemical Agent Co., Inc. (Tianjin, China). Acetonitrile and methanol (HPLC-grade) were purchased from Fisher Scientific (Pittsburgh, USA). All aqueous solutions were prepared using ultrapure water which is produced by MK-459 Millipore Milli-Q Plus ultra-pure water system (Shandong, China). MTT, dulbecco’s modified eagle medium (DMEM) and trypsin were obtained from Sigma (Saint Louis, USA).
3
Quantification of Milk Proteins and Histamine
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bovine αs-casein (α-CN), bovine β-casein (β-CN), bovine α-lactalbumin (α-Lac), bovine β-lactoglobulin A (β-LgA), bovine β-lactoglobulin B (β-LgB), histamine, urea, and n-octyl-β-D-glucopyranoside (OG) were from Sigma-Aldrich (St. Louis, MO, USA). Trifluoroacetic acid (TFA) and acetonitrile (ACN) were from Tedia Company (Fairfield, OH, USA). All other reagents were of analytical grade or higher grade. Silica gel (ZEX-II, 5 µm, 300 Å) was from Qingdao Meigao Chemical Co., Ltd. (Qingdao, China). All aqueous solutions were prepared using ultrapure water from an MK-459 Millipore Milli-Q Plus ultrapure water system. Commercial infant milk powder samples were from local shops.
Stock standard solutions of α-CN, α-Lac, β-CN, β-LgB, and β-LgA were prepared in a buffer consisting of 6 M urea and 0.2% OG (pH 3.3) [13] (link), at concentrations of 10 mg/mL. Working solutions containing all analytes were % mM KCl, 2.54 mM CaCl2, 1.19 mM KH2PO4, 10 mM HEPES, 5 mM glucose, 0.1% (w/v) BSA, pH 7.3). Stock internal standard solutions of histamine-d4 at 30 ng/mL were prepared in ACN.
Stock standard solutions of α-CN, α-Lac, β-CN, β-LgB, and β-LgA were prepared in a buffer consisting of 6 M urea and 0.2% OG (pH 3.3) [13] (link), at concentrations of 10 mg/mL. Working solutions containing all analytes were % mM KCl, 2.54 mM CaCl2, 1.19 mM KH2PO4, 10 mM HEPES, 5 mM glucose, 0.1% (w/v) BSA, pH 7.3). Stock internal standard solutions of histamine-d4 at 30 ng/mL were prepared in ACN.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!