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Multiplexed fluorometric immunoassay

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The Multiplexed fluorometric immunoassay is a laboratory equipment used for the simultaneous detection and quantification of multiple analytes in a single sample. It utilizes the principle of fluorescence-based detection to measure the concentration of various target molecules, such as proteins, cytokines, or metabolites, in a complex biological matrix.

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Lab products found in correlation

Pcr rodent infectious agent array methods, by Charles River Laboratories (2 mentions) Ez spot, by Charles River Laboratories (2 mentions)

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Multiplexed Fluorometric Immunoassay (MFIA, (2 citations)

2 protocols using multiplexed fluorometric immunoassay

1

Comprehensive Murine Pathogen Screening

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Serum was collected at the indicated times for antibody screening using EZ-spot followed by a multiplexed fluorometric immunoassay (Charles River). The screening panel includes: mouse hepatitis virus (MHV), Sendai virus, pneumonia virus of mice, minute virus of mice (MVM), mouse parvovirus type 1(MPV), mouse parvovirus type 2, mouse parvovirus-NS1, murine norovirus (MNV), Theiler’s murine encephalomyelitis virus (TMEV), reovirus, rotavirus EDIM, lymphocytic choriomeningitis virus, ectromelia virus, mouse adenovirus 1 and 2, mouse cytomegalovirus, polyoma virus, Mycoplasma pulmonis, Enchephalitozoon cuniculi, cilia-associated respiratory bacillus, and Clostridium piliforme. Relative serology scores for MHV antigens (recombinant A59-strain nucleocapsid protein, purified A-59 viral lysate, and purified S-strain viral lysate) were calculated by Charles River using median fluorescence index. Active pathogen infection was measured by PCR Rodent Infectious Agent (PRIA) array methods (Charles River) in samples collected from oral swabs or fecal material. This panel screened for MHV, MNV, MPV, MVM, Rodentibacter heylii, and Helicobacter species.
Camell C.D., Yousefzadeh M.J., Zhu Y., Prata L.G., Huggins M.A., Pierson M., Zhang L., O’Kelly R.D., Pirtskhalava T., Xun P., Ejima K., Xue A., Tripathi U., Espindola-Netto J.M., Giorgadze N., Atkinson E.J., Inman C.L., Johnson K.O., Cholensky S.H., Carlson T.W., LeBrasseur N.K., Khosla S., O’Sullivan M.G., Allison D.B., Jameson S.C., Meves A., Li M., Prakash Y.S., Chiarella S.E., Hamilton S.E., Tchkonia T., Niedernhofer L.J., Kirkland J.L, & Robbins P.D. (2021). Senolytics reduce coronavirus-related mortality in old mice. Science (New York, N.y.), 373(6552), eabe4832.
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2

Comprehensive Pathogen Screening Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum was collected at the indicated times for antibody screening using EZ-spot followed by a multiplexed fluorometric immunoassay (Charles River). The screening panel includes: mouse hepatitis virus (MHV), Sendai virus, pneumonia virus of mice, minute virus of mice (MVM), mouse parvovirus type 1(MPV), mouse parvovirus type 2, mouse parvovirus-NS1, murine norovirus (MNV), Theiler’s murine encephalomyelitis virus (TMEV), reovirus, rotavirus EDIM, lymphocytic choriomeningitis virus, ectromelia virus, mouse adenovirus 1 and 2, mouse cytomegalovirus, polyoma virus, Mycoplasma pulmonis, Enchephalitozoon cuniculi, cilia-associated respiratory bacillus, and Clostridium piliforme. Relative serology scores for MHV antigens (recombinant A59-strain nucleocapsid protein, purified A-59 viral lysate, and purified S-strain viral lysate) were calculated by Charles River using median fluorescence index. Active pathogen infection was measured by PCR Rodent Infectious Agent (PRIA) array methods (Charles River) in samples collected from oral swabs or fecal material. This panel screened for MHV, MNV, MPV, MVM, Rodentibacter heylii, and Helicobacter species.
Camell C.D., Yousefzadeh M.J., Zhu Y., Prata L.G., Huggins M.A., Pierson M., Zhang L., O’Kelly R.D., Pirtskhalava T., Xun P., Ejima K., Xue A., Tripathi U., Espindola-Netto J.M., Giorgadze N., Atkinson E.J., Inman C.L., Johnson K.O., Cholensky S.H., Carlson T.W., LeBrasseur N.K., Khosla S., O’Sullivan M.G., Allison D.B., Jameson S.C., Meves A., Li M., Prakash Y.S., Chiarella S.E., Hamilton S.E., Tchkonia T., Niedernhofer L.J., Kirkland J.L, & Robbins P.D. (2021). Senolytics reduce coronavirus-related mortality in old mice. Science (New York, N.y.), 373(6552), eabe4832.
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