Luna c18 column

Analytical HPLC analyses were performed on a Shimadzu 10A model HPLC system (Shimadzu Analytical and Measuring Instruments, Kyoto, Japan) with a pump (LC-10AD), a diode-array detector (DAD) (SPD-M10A), and an autosampler (SIL-10AD). A Luna C18 column (250 × 4.60 mm, 5 micron; Phenomenex, Torrance, CA, USA) was used for the comparison of the dichloromethane extract of Ferula huber-morathii with reference compounds ferutinin (17) and elaeochytrin A (18) (isolated from Ferula elaeochytris Korovin) and cytotoxic sesquiterpene coumarin ethers of F. huber-morathii (i.e., compounds 1–15) (Supplementary Materials Figure S40).
A Gilson PLC 2050 (Saint-Avé, France) preparative HPLC system with a Luna C18 column (150 × 21.2 mm, 5 micron; Phenomenex, Torrance, CA, USA) was used for the preparative HPLC purifications.
Supelco Silica gel 60 F₂₅₄ PTLC plates (0.5, 1, and 2 mm; Merck, Darmstadt, Germany) were used for the preparative separations. Silica gel 60 F254 TLC plates (0.25 mm; Merck, Darmstadt, Germany) were used for the analytical separations. A UV lamp (Camag, Muttenz, Switzerland) with 254 and 366 nm wavelength detection capabilities was used for the visualization of plates. Compounds without chromophore groups were detected by spraying plates with freshly prepared 10% p-anisaldehyde in 10% ethanolic sulfuric acid, followed by heating.

Ramulus cinnamomi (RC) were bought in June 2013 from Zhangshu medicinal market, Jiangxi Province, China, and authenticated by Prof Shouran Zhou (College of Basic Medicine, Jiangxi University of Traditional Chinese Medicine). A voucher specimen (no. RC-201306) was deposited in the herbarium of Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables, Jiangxi Agricultural University (Jiangxi, China).
Penicillium italicum, Penicillium digitatum, Geotrichum candidum Link, Colletotrichum gloeosporioides (Penz.), Alternaria citri, and Phytophthora spp. were provided by the Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables (Nanchang, China). All the test strains were preserved on potato dextrose agar.
¹H- and ¹³C-NMR spectral data were tested on a Varian 400 MHz Nuclear magnetic resonance spectrometer. HPLC was conducted on a Hitachi Elite 2100 system, Luna C18 column (5 µm, 4.6 mm × 250 mm) for analysis and Luna C18 column (5 µm, 10 mm × 250 mm) for semi-preparative HPLC were purchased from Phenomenex Inc. The HPLC grade solvents were purchased from Sigma (Sigma, USA). All analytical solvents were bought from Tansoole (Shanghai, China).

Peptides were analyzed and purified using RP-HPLC. 0.1% TFA in deionized (d.i.) water and 80% ACN, 0.1% TFA in d.i. water were used as eluent A and eluent B, respectively.
Analytical HPLC experiments were performed on a Hewlett-Packard Agilent 1100 Series HPLC apparatus using a Luna C18 column (100 Å, 5 μm, 250 × 4.60 mm, Phenomenex, Aschaffenburg, Germany), at a flow rate of 1.2 mL∙min⁻¹ with a gradient of 0 to 25% B over 25 min.
Preparative chromatography was carried out on a Shimadzu HPLC instrument equipped with a Luna C18 column (100 Å, 10 μm, 250 × 21.2 mm, Phenomenex, Aschaffenburg, Germany) at a flow rate of 4 mL∙min⁻¹. The gradient was 0 to 30% B over 60 min for P33, 30% to 60% B over 100 min for Fe65-WW, 20% to 60% B over 120 min for Pin1-WW, and 0 to 40% B over 80 min for PPPPP.

LF was dispersed in 50% EtOH (10 mg/mL) by vortex mixer. Dispersed solutions were sonicated for 10 min and centrifuged. The supernatant was used for HPLC analysis. AM was dissolved in 70% EtOH (5 mg/mL) and directly used for HPLC analysis. All samples were filtered using 0.45 μm nylon syringe filter before injecting it into the HPLC system.
Chromatographic separations of caffeoylquinic acids (CQAs) were achieved using a Phenomenex Luna C18 column (250 mm × 4.6 mm I.D, 100 Å). A reverse phase HPLC was carried out by gradient conditions, and the flow rate of mobile phase was 1.0 mL/min. Mobile phases were composed in 0.1% triflouroacetic acid (TFA) in deionized water (A) and methanol (B). The gradient program started in 15% B and with the following composition: 0–3 min, 15% B; 3–7 min, 19% B; 7–15 min, 19% B; 15–28 min, 32% B; 28–33 min, 90% B; 33–38, 15% B; and 38–43, 15% B. Column oven was set at 30°C, and the detection of CQAs was performed at 330 nm on a UV detector.
C3G was determined by separating with a Phenomenex Luna C18 column (150 mm × 4.6 mm, 100 Å), and the mobile phases were composed of 0.4% of TFA in deionized water and 0.4% of TFA in 50% MeOH. The gradient program begun in 0% B: 0–2 min, 70%; 20–22 min, 100%, 22–30 min, 0% B; 30–35 min, 0% B. Temperature of separation was set at 30°C, and the detection wavelength was 520 nm.