Prism software version

Statistical significance was calculated using the log-rank test, one-way ANOVA with a Turkey’s multiple comparison test, and two-way repeated-measure ANOVA by Prism software version 4.0a (GraphPad Software). p < 0.05 was considered statistically significant.

All results from the present study were collected by experimenters blinded to treatment conditions and results were reported as the mean±s.d. for immunoblots. For behavioral and biochemical analyses, values were expressed as the mean±s.e.m. Comparisons among multiple groups were analyzed using one-way ANOVA followed by Tukey's post hoc test, and statistical analysis was performed using Prism software version 6 (GraphPad Software, San Diego, CA, USA). Significant differences were defined as P-value <0.05.

All data were subjected to statistical analysis using Prism software version 7 (GraphPad). Comparisons between two groups were performed by 2-tailed t-test (parametric) or paired t-test (non-parametric). Comparisons between multiple groups were performed using a 1-way ANOVA with Bonferroni's multiple comparison test. A P < 0.05 was considered significant.

Numerical data were statistically analyzed using Prism software version 5.0 (Graphpad Software, USA). All parametric data is presented as the mean ± SEM and analyzed using Student's t test or ANOVA with Bonferroni's post‐test adjustment for multiple comparisons where appropriate. Differences were considered significant at P < 0.05. Recovery of gait was analyzed using a summary measures approach for weekly repeated measurements on each animal. For each individual animal, measurements of gait symmetry, stride length and stride frequency for weeks 4–8 (period of accelerated recovery after cell transplantation) were analyzed in Minitab using linear regression analysis to generate a linear coefficient value. The linear coefficient values were compared using the Mann‐Whitney test to assess the equality of the slopes between control and transplanted groups for each gait parameter (Matthews, 1993; Vossoughi et al., 2012).

Statistical significance between groups was determined by the two-tailed unpaired Student’s t-test or One-way ANOVA with Dunnett or Tukey post-test using Prism software version 5.03 (GraphPad).

Data is presented as mean ± SEM. Data analysis was carried out with Prism Software version 8 (Graph Pad, San Diego, California, U.S.A.). Student´s T-test was used when only two groups were compared. To evaluate differences between more three or more groups, Two-way Analysis of Variance (ANOVA) followed by Sidak´s Multiple Comparison Test was applied. The results were considered statistically significant at p ≤ 0.05.

For all experiments, statistics were performed with Prism software version 5.0 (GraphPad, La Jolla, CA). An independent Student's t-test was used to determine statistical differences between experimental and control values. The probabilities of overall survival were calculated using the Kaplan–Meier method and were compared using the log-rank test. For determining factors related to overall survival, a Cox proportional hazard model was utilized. Institutional Review Board of Tuskegee University approved the use of patient specimens for this study. Microarray arrays analysis was performed using SAS for Windows (Version 9.2; SAS Institute, Cary, NC). P values < 0.05 were considered statistically significant.