Reagents of analytical grade were mainly used, with the exception of mobile-phase reagents, which comprised solvents of high-performance liquid chromatography (HPLC) grade. Analytical standards of MPA and NIV were purchased from Supelco (Bellefonte, PA, USA); of PA, from Santa Cruz Biotechnology (Dallas, TX, USA); and of AFB1, AFB2, AFG1, AFG2, AFM1, BEA, CIT, DON, ENNA, ENNB, FB1, FB2, HT-2, T-2 toxins, MON, OTA, PAT, TEA, TTX, and ZEA from Sigma-Aldrich (Steinheim, Germany). Ultrapure H2O was supplied through a Milli-Q water system from Merck (Saint-Quentin-Fallavier, France) and C18 sorbent from Agilent Technologies (Santa Clara, CA, USA). Acetonitrile (ACN) was acquired from Carlo Erba (Val de Reuil, France), anhydrous magnesium sulfate (MgSO4) and sodium chloride (NaCl) from Honeywell (Seelze, Germany), and formic acid from Chem-Lab (Zedelgem, Belgium). ACQUITY UPLC® HSS T3 1.8 μm (2.1 × 100 mm i.d.) was obtained from Waters (Milford, MA, USA); HPLC vials and Syringeless Device Mini UniPrep filters (0.45 µm PVDF, polypropylene), from Whatman (Maidstone, England).
C18 sorbent
Manufactured by Agilent Technologies
Sourced in United States, France
The C18 sorbent is a type of solid-phase extraction (SPE) media. It is composed of silica particles that have been chemically modified with C18 alkyl chains. The primary function of the C18 sorbent is to selectively retain and separate analytes from complex matrices based on their hydrophobic interactions with the C18 alkyl chains.
Automatically generated - may contain errors
Lab products found in correlation
Anhydrous magnesium sulfate mgso4 and sodium chloride nacl, by Honeywell (2 mentions)
Acquity uplc hss t3 1.8 μm, by Waters Corporation (2 mentions)
Syringeless device mini uniprep filters, by Cytiva (2 mentions)
Formic acid, by Chem-Lab (2 mentions)
Milli q water system, by Merck Group (1 mentions)
Hplc vials, by Cytiva (1 mentions)
Acetonitrile, by Honeywell (1 mentions)
Ammonium carbonate, by Avantor (1 mentions)
Acetonitrile, by Avantor (1 mentions)
Methanol, by Avantor (1 mentions)
Diatomaceous earth, by Merck Group (1 mentions)
Anhydrous sodium sulfate, by ITW Reagents (1 mentions)
Milli q gradient a10 system, by Merck Group (1 mentions)
Florisil 60 100 mesh, by Merck Group (1 mentions)
Silica gel 60, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
N hexane, by Merck Group (1 mentions)
Milli q gradient water system, by Merck Group (1 mentions)
6 protocols using c18 sorbent
1
Multi-Mycotoxin Analysis Protocol
2
Analytical Grade Solvent Extraction
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All reagents
were of analytical reagent grade, and solvents were of LC–MS
grade. Acetonitrile (MeCN), methanol (MeOH), ammonium carbonate, and
formic acid were supplied by VWR International (Milan, Italy). Z-sep+
sorbent for cleanup was obtained from Supelco (Bellefonte, PA, USA),
while the C18 sorbent was supplied by Agilent Technologies (USA).
were of analytical reagent grade, and solvents were of LC–MS
grade. Acetonitrile (MeCN), methanol (MeOH), ammonium carbonate, and
formic acid were supplied by VWR International (Milan, Italy). Z-sep+
sorbent for cleanup was obtained from Supelco (Bellefonte, PA, USA),
while the C18 sorbent was supplied by Agilent Technologies (USA).
3
Solid-Phase Extraction of Plasma Steroids
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Overall solid-phase extraction procedures were performed according to manufacturer’s instruction with slight modification. Two hundred microliter of collected plasma were transferred into 2 mL centrifuge tube, followed by addition of 5 µL of internal standard (IS) solutions (1 µg/mL). Then, plasma samples were diluted with 800 µL of 0.2 M acetate buffer at pH 5.2. SPE cartridges filled with C18 sorbent (Agilent, Santa Clara, CA, USA), which were sequentially pre-conditioned with 1 mL of MeOH and 1 mL of DW. After sample loading, the SPE cartridges were washed with 1 mL of DW, followed by dryness under vacuum (–30 kPa). Finally, all target steroids were eluted from dried SPE sorbents using 1 mL of EA.
4
Analytical Standards for Mycotoxin Detection
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Reagents of analytical grade were mainly used, with the exception of mobile phase reagents which were of high-performance liquid chromatography (HPLC) grade. Analytical standards comprised: MPA and NIV from Supelco (Bellefonte, PA, USA); PA from Santa Cruz Biotechnology (Dallas, TX, USA); AFB1, B2, G1, G2, and M1, BEA, CIT, DON, ENNA, and B, FB1 and B2, HT-2 and T-2 toxins, MON, OTA, PAT, TEA, TTX, and ZEA from Sigma-Aldrich (Steinheim, Germany). Ultrapure H2O was supplied from Millipore System (Paris, France), C18 sorbent from Agilent Technologies (Santa Clara, CA, USA), acetonitrile (ACN) from Carlo Erba (Val de Reuil Cedex, France), anhydrous magnesium sulfate (MgSO4) and sodium chloride (NaCl) from Honeywell (Seelze, Germany), and formic acid from Chem-Lab (Zedelgem, Belgium). ACQUITY UPLC® HSS T3 1.8 μm (2.1 × 100 mm i.d.) was purchased from Waters (Milford, MA, USA), and HPLC vials and Syringeless Device Mini UniPrep filters (0.45 μm PVDF, polypropylene) from Whatman (Maidstone, UK).
5
Solvent Extraction and Cleanup Protocol for Pharmaceutical Compounds
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Acetonitrile (ACN), methanol (MeOH) (gradient-grade, Lichrosolv), n-hexane, acetone, ethyl acetate (EtOAc) and dichloromethane (DCM) (Suprasolv) were purchase from Merck (Darmstadt, Germany). Ultrapure water was produced by means of a Milli-Q gradient A-10 system (Millipore, Billerica, MA, USA). The
and N-desethylamiodarone
) and ketoprofen propanoic acid) standards were obtained from Sigma-Aldrich (Madrid, Spain). Potassium hydroxide (Pellets, 85%+, AC)
was also purchased to Sigma-Aldrich, and sodium sulfate anhydrous was obtained from Panreac (Barcelona, Spain). Florisil (60-100 mesh) and silica bonded to ethylenediamine-N-propyl groups (PSA sorbent) were purchased from Supelco (Bellefonte, PA, USA). Diatomaceous earth was provided by Sigma-Aldrich and silica bonded to C18 (C18 sorbent) was purchased to Agilent Technologies (Santa Clara, CA, USA). Silica gel 60 (0.040-0.063 mm) was obtained from Merck. For some experiments, Florisil and silica gel were activated at 120 ºC for at least 12 h and then allowed to cool at room temperature in a desiccator before use.
MSPD empty polypropylene syringes (15 mL capacity) and 20 µm polyethylene frits were acquired from International Sorbent Technology (Mid Glamorgan, UK).
and N-desethylamiodarone
) and ketoprofen propanoic acid) standards were obtained from Sigma-Aldrich (Madrid, Spain). Potassium hydroxide (Pellets, 85%+, AC)
was also purchased to Sigma-Aldrich, and sodium sulfate anhydrous was obtained from Panreac (Barcelona, Spain). Florisil (60-100 mesh) and silica bonded to ethylenediamine-N-propyl groups (PSA sorbent) were purchased from Supelco (Bellefonte, PA, USA). Diatomaceous earth was provided by Sigma-Aldrich and silica bonded to C18 (C18 sorbent) was purchased to Agilent Technologies (Santa Clara, CA, USA). Silica gel 60 (0.040-0.063 mm) was obtained from Merck. For some experiments, Florisil and silica gel were activated at 120 ºC for at least 12 h and then allowed to cool at room temperature in a desiccator before use.
MSPD empty polypropylene syringes (15 mL capacity) and 20 µm polyethylene frits were acquired from International Sorbent Technology (Mid Glamorgan, UK).
6
Multi-Analyte Extraction and Analysis
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Paclobutrazol (PA), 6-benzyladenine (6-BA), indole-3-acetic acid (IAA), 3-indolepropionic acid (IPA), 3-indolebutyric acid (IBA), tetrahydropyranyl benzyladenine (PBA), kinetin (6-KT), chlormequat chloride (CCC), uniconazole (UN), daminozide (B9), zeatin (ZT), mepiquat chloride (Pix), gibberellic acid (GA3), 2,4-dichlorophenoxyacetic acid (2,4-D), thidiazuron (TDZ), forchlorfenuron (CPPU), 4-chlorophenoxyacetic acid (4-CPA), 2,3,5-triiodobenzoic acid (2,3,5-TIBA) and 2-naphthoxyacetic acid (2-NOA) were supplied by Dr. Ehrenstorfer GmbH (Augsburg. Germany). 3-Indolepropionic-d2 acid and forchlorfenuron-d5 were purchased from CDN (Canada). Acetonitrile and acetic acid (optima ® LC/MS) were purchased from Fisher Science. Ammonium acetate was purchased from CNW (Germany). Magnesium sulfate, sodium acetate and sodium chloride were purchased from Beijing Chemical Reagents Factory (Beijing, China). C18 sorbent was purchased from Agilent (USA). Ultrapure water was obtained through Milli-Q gradient water system (Millipore, Bedford, MA, USA). Matrix solid phase dispersion-extraction pipe (adding 100 mg magnesium sulfate anhydrous plus 50 mg C18) were purchased from Waters (USA).
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