N protein was manufactured by Sino Biological Inc. (Beijing, China). Epichlorohydrin, sodium hydroxide, isopropanol, tris hydroxymethyl aminomethane, and toluene were provided by China National Pharmaceutical Group Co., Ltd. (Beijing, China). DNAs were bought from TaKaRa Bio. Inc. (Dalian, China). Serum albumin was brought from Shandong Sikejie Biotechnology Co., Ltd. (Binzhou, China) β-cyclodextrin was supplied by Sigma–Aldrich Co., Ltd. (Shanghai, China). T4 polynucleotide kinase, lysozyme, Exo I, and 10 × Exo I buffer (67 mM MgCl2, 10 mM dithiothreitol, pH = 9.5, 670 mM glycine-KOH) were provided by Sangon Biotech Co. Ltd. (Shanghai, China). Fibrinogen, immunoglobulin G, hemoglobin, amylase, and cytochrome C were supplied by Beijing Soleibo Technology Co., Ltd. (Beijing, China). Alkaline phosphatase was purchased from Shanghai Biyuantian Biological Co., Ltd. (Shanghai, China). Sialomucin was obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Two electroneutral β-CDP (one of the molecular weights was 0.3885 KD, and the other was water insoluble) were bought from Shandong Zhiyuan Biotechnology Co., Ltd. (Binzhou, China).
Isopropanol
Manufactured by Sinopharm
Sourced in China
Isopropanol, also known as isopropyl alcohol, is a colorless, flammable liquid. It is a common laboratory reagent used as a solvent, disinfectant, and cleaning agent. Isopropanol has a wide range of applications in various industries, including pharmaceuticals, cosmetics, and electronics.
Automatically generated - may contain errors
Lab products found in correlation
Ethanol, by Sinopharm (34 mentions)
Methanol, by Sinopharm (22 mentions)
Sodium hydroxide (naoh), by Sinopharm (19 mentions)
Acetone, by Sinopharm (18 mentions)
Hydrochloric acid (hcl), by Sinopharm (13 mentions)
Sodium chloride (nacl), by Sinopharm (12 mentions)
Chloroform, by Sinopharm (11 mentions)
Toluene, by Sinopharm (8 mentions)
Anhydrous ethanol, by Sinopharm (8 mentions)
Acetonitrile, by Sinopharm (8 mentions)
Potassium hydroxide, by Sinopharm (7 mentions)
Glycerol, by Sinopharm (7 mentions)
Ethylene glycol, by Sinopharm (6 mentions)
Sodium borohydride, by Sinopharm (5 mentions)
Hexane, by Sinopharm (5 mentions)
P benzoquinone, by Sinopharm (5 mentions)
Absolute ethanol, by Sinopharm (5 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (5 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
N hexane, by Sinopharm (4 mentions)
129 protocols using isopropanol
1
Protein Expression and Purification Protocol
2
Isocarbophos-Chlorpyrifos DNA Aptamer Synthesis
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Isocarbophos (99%, purity) was brought from
Shanghai Pesticide Research Institute (Shanghai, China), and chlorpyrifos
(98%) was provided by Sinopharm Chemical Reagent Co., Ltd. (Shanghai,
China). Acetone was purchased from Xilong Scientific Co., Ltd. (Shantou,
China). A 3′-pyrene-labeled isocarbophos DNA aptamer (5′-AGCTTGCTGCAGCGATTCTTGATCGCCACAGAG
CT-3′) was synthesized by TaKaRa Bio. Inc. (Dalian, China).
β-Cyclodextrin was bought from Sigma-Aldrich Co., Ltd. Tris
hydroxymethyl aminomethane, toluene, epichlorohydrin, isopropanol,
and sodium hydroxide were obtained from China National Pharmaceutical
Group Co., Ltd. 10× Exo I buffer (67 mM MgCl2, 670
mM glycine–KOH, 10 mM dithiothreitol, pH = 9.5) and Exo I were
provided by Sangon Biotech Co., Ltd. (Shanghai, China). The ultrapure
water was provided by a Millipore water purification system (≥18.2
MΩ cm).
Shanghai Pesticide Research Institute (Shanghai, China), and chlorpyrifos
(98%) was provided by Sinopharm Chemical Reagent Co., Ltd. (Shanghai,
China). Acetone was purchased from Xilong Scientific Co., Ltd. (Shantou,
China). A 3′-pyrene-labeled isocarbophos DNA aptamer (5′-AGCTTGCTGCAGCGATTCTTGATCGCCACAGAG
CT-3′) was synthesized by TaKaRa Bio. Inc. (Dalian, China).
β-Cyclodextrin was bought from Sigma-Aldrich Co., Ltd. Tris
hydroxymethyl aminomethane, toluene, epichlorohydrin, isopropanol,
and sodium hydroxide were obtained from China National Pharmaceutical
Group Co., Ltd. 10× Exo I buffer (67 mM MgCl2, 670
mM glycine–KOH, 10 mM dithiothreitol, pH = 9.5) and Exo I were
provided by Sangon Biotech Co., Ltd. (Shanghai, China). The ultrapure
water was provided by a Millipore water purification system (≥18.2
MΩ cm).
3
RNA Extraction Protocol for Samples
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Reagents used in the study are listed in Table I , while the equipment used are listed in Table II . Other chemical agents, including diethylpyrocarbonate (DEPC)-treated water, chloroform, anhydrous ethanol, isopropanol and EDTA, were purchased from Sinopharm Chemical Reagent Co., Ltd., (Shanghai, China).
4
Oolong Tea Leaf Lipid Extraction and Analysis
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Isopropanol, chloroform, methanol, potassium chloride, sulfuric acid and toluene all were analytically pure (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China). N-hexane, Supelco 37 Component FAME Mix (Table S1 ), α-linolenic, linoleic, palmitic, oleic acid and other referencing standards were chromatographically pure (Sigma, Saint Louis, MO, USA). LOX activity detection kit (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) was used for the measurement of enzyme activity in oolong tea leaves.
5
Antimicrobial Textile Coating Evaluation
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Polyester staple fibers and draw textured yarn (DTY) were acquired from Jiangsu Chemical Fiber Co., Ltd., Wuxi, China. Biocompatible polyacrylate coating latex was supplied by Shanghai Huasen Chemical Co., Ltd.,Shanghai, China. Commercial medical gauzes from Winner Industries Co., Ltd., Shenzhen, China, were employed as control samples. Staphylococcus aureus (ATCC 25923) was provided by JK Microorganism Co., Ltd., China. Tryptic soy broth (TSB) was prepared with tryptone Lp0042, yeast extract Lp0041 (OXOID, Britain), and sodium chloride (Shanghai Lingfeng Chemical Reagent Co., Ltd., Shanghai, China). Methylthiazolyldiphenyl-tetrazolium bromide, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) was purchased from Shanghai Bio-Gene Technology Co., Ltd., Shanghai, China. Fluorescein isothiocyanate (FITC) and propidium iodide (PI) were obtained from Sigma, USA. CaCl2, NaOH, glutaraldehyde, ethanol, and isopropanol were all purchased from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China. All chemicals were analytical grade and were prepared with deionized water (DIW).
6
Synthesis and Characterization of Halide Perovskites
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Phenylethylamine bromide (PEABr, 99.9%), phenylethylamine iodide (PEAI, 99.9%), and n-Butylamine hydrobromide (BABr, 99.9%) were all purchased from Advanced Election Technology Co., Ltd. Lead bromide (PbBr2, 99.998%) and lead (II) iodide (PbI2, 99.999%) were purchased from Alfa Aesar. Hydrobromic acid (HBr, 40% wt/wt aq. sol.), N, N-Dimethylformamide (≥99.5%), acetone (≥99.5%), ethanol (75%), and isopropanol (≥99.7%) were all purchased from Sinopharm Group Co., Ltd. All the above reagents were used as received.
7
Comprehensive ELISA and RT-PCR Protocol
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The PCIII ELISA kit (Shanghai Huzheng Biotechnology Co., Ltd., Shanghai, China), IV-C and LN ELISA kits (Nanjing SenBeiJia Biological Technology Co., Ltd., Nanjing, China), HA and AST ELISA kits (Shanghai Jianglai Biotechnology Co., Ltd., Shanghai, China), ALT, TG, and TC ELISA kits (Shanghai Enzyme Biotechnology Co., Ltd., Shanghai, China), SYBR Green PCR Master Mix kit (Applied Biology, Irvine, CA, USA), Fermentas K1622 RT reverse transcription kit (Massachusetts Biomedical Initiatives, Worcester, MA, USA), absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China), chloroform (Shanghai Shenggong Co., Ltd., Bioengineering, Shanghai, China), isopropanol (Sinopharm Group Chemical Reagent Co.), TRIzol (Invitrogen, Carlsbad, CA, USA), SDS-PAGE gel compounding reagent box (Biyuntian Biotechnology Co., Ltd., Shanghai, China), rabbit antihepatitis B virus pre-X protein antibody, and goat anti-rabbit IgG H and L (HRP) (Abcam, Cambridge, UK) were used in this study.
8
Fabrication of Silver-Graphene Electrochemical Sensors
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Silver nitrate, sodium citrate, sodium borohydride, sodium chloride, isopropanol, and ethanol were analytical grade and purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). From Aladdin Reagent Inc. (Shanghai, China), 60 wt.% PTFE was purchased. Industrial-grade CNTs (outer diameter of 50 nm, inner diameter of 20~30 nm, and length of 10~30 μm) were supplied by Tanfeng Graphene Technology Co. Ltd. (Suzhou, China). Commercial 2 μm silver powders were purchased from the Beijing Nonferrous Metals Research Institute (Beijing, China). Ketjenblack (KB), Super P and KS-6 commercial conductive carbon black were supplied by the Wuhan Battery Factory (Wuhan, China). Sintered Ag/AgCl electrodes (outer diameter of 12 mm, inner diameter of 6 mm, and thickness of 1 mm) and GT10 conductive gels (~3.0 wt.% NaCl) were bought from Wuhan Greentek Pty. Ltd. (Wuhan, China).
9
Synthesis of Molybdenyl Acetylacetonate
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Molybdenyl acetylacetonate (MoO2(acac)2, 97%) was purchased from Sigma-Aldrich Co., Ltd. (Shanghai, China) Ethanol. D-(+)-glucose (AR) and isopropanol (IPA) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All the reagents were analytical grade and used without further purifications. Ultrapure water (18.25 MΩ·cm) was used throughout the experiments.
10
Fatty Acid Methyl Esters Analysis
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SPO was purchased from Qinghai Kangpu Co., Ltd. (Xining, Qinghai) . Standards of 37 fatty acid methyl esters (FAMEs) were purchased from Sigma-Aldrich Chemical Co., Ltd. (Shanghai, China) . Hexane, methanol, ethanol, acetone and isopropanol were provided by Sinopharm Chemical Regent (Shanghai, China) . All of the other reagents were analytically pure and were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China) .
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