Ab1793

Manufactured by Abcam

Sourced in United States, United Kingdom

Ab1793 is a protein that binds to a specific target. It can be used for various research applications.

Automatically generated - may contain errors

Lab products found in correlation

Ab9722, by Abcam (12 mentions) Ab6672, by Abcam (6 mentions) Ab86299, by Abcam (5 mentions) Ab32536, by Abcam (4 mentions) Two step detection kit, by Zhongshan Golden Bridge Biotechnology (3 mentions) Ab290735, by Abcam (3 mentions) Ab14519, by Abcam (3 mentions) Ab170874, by Abcam (3 mentions) Prolong diamond antifade reagent, by Thermo Fisher Scientific (3 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (3 mentions) Cyanine3 (cy3), by Thermo Fisher Scientific (3 mentions) Ab6721, by Abcam (3 mentions) Ab150113, by Abcam (3 mentions) Ab16502, by Abcam (3 mentions) Lsm 710, by Zeiss (3 mentions) Ab125066, by Abcam (2 mentions) Ab150077, by Abcam (2 mentions) Ab182981, by Abcam (2 mentions) Ma5 14568, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions)

71 protocols using ab1793

Protein Expression Analysis in Cells

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Tissue was homogenized, lysed and centrifuged at 2000 rpm for 20 mins. The supernatants were collected and protein concentrations were measured using BAC kits (Keygen Biotech, Nanjing, People’s Republic of China). Proteins were separated through 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (25 µg/lane), and membranes prepared by Western blot procedure were finally analyzed using the ECL system (Millipore, Bedford, USA). Primary antibodies were used as follows:
GAPDH (#8884, 1:10000, Cell Signaling Technology, Boston USA), Caspase-3 (#9662, 1: 1000, Cell Signaling Technology, Boston USA), Cleaved Caspase-3 (#9664, 1:1000, Cell Signaling Technology, Boston USA), Bax (#5023, 1:1000, Cell Signaling Technology, Boston USA), Bcl-2 (#15071, 1:1000, Cell Signaling Technology, Boston USA), HIF-1α (#36169, 1:1000, Cell Signaling Technology, Boston USA), VEGF (ab52917, 1:1000, Abcam, England), TNF alpha (ab1793, 1:1000, Abcam, England), IL-1 beta (ab9722, 1:2000, Abcam, England).

Zhang L., Liu J., Ge Y, & Liu M. (2019). Ginkgo biloba Extract Reduces Hippocampus Inflammatory Responses, Improves Cardiac Functions And Depressive Behaviors In A Heart Failure Mouse Model. Neuropsychiatric Disease and Treatment, 15, 3041-3050.

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Immunohistochemical Analysis of MDA and TNF-α

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The sections were prepared and mounted on electrostatic slides. Sections were deparaffinized in xylene and rehydrated in descending concentration of ethanol and finally in distilled water. Sections were heated in citrate buffer (pH = 6) within microwave for 5 minutes and washed with PBS buffer for 5 minutes. Sections were incubated in peroxidase blocking solution for 10 minutes and incubated overnight at 4°C in diluted primary antibodies (1 : 1000) anti-malondialdehyde (ab194225, Abcam) and anti-TNF-α antibody (ab1793, Abcam). Sections were rinsed with PBS buffer 3 times each for 5 minutes and incubated with biotinylated goat anti-mouse (ab128976) for 30 minutes at room temperature, followed by incubation in avidin-biotin complex for 30 min. The sections were then rinsed 3 times with PBS each for 5 minutes and incubated in DAB solution for 10 minutes. After that, Mayer's hematoxylin was added. Finally, the sections were dehydrated using ascending concentrations of ethanol and cleared with two washes of xylene. Sections were mounted, and images were taken at 400x. Images were analyzed using the Fiji software to calculate the optical density using the formula OD = log (max intensity/mean intensity), where the max intensity = 255 for 8-bit images.

Alhusayan R.M., Aldahmash B.A., El-Nagar D.M., Rady A., Ibrahim K.E, & Alkahtani S. (2020). Butterbur (Petasites hybridus) Extract Ameliorates Hepatic Damage Induced by Ovalbumin in Mice. Oxidative Medicine and Cellular Longevity, 2020, 3178214.

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Comprehensive Molecular Profiling of Samples

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For immunohistochemistry, immunofluorescence, and Western blot, the following antibodies were used according to the manufacturers' instructions: mouse anti-TNF alpha (TNF-α, ab1793; Abcam), rabbit anti-IFN-γ (bs-0480R; Bioss), anti-MPO (22225-1-AP; Proteintech), anti-alpha smooth muscle actin (α-SMA, ab124964; Abcam), anti-transferrin receptor (TFR; ab214039; Abcam), anti-GPX4 (ab125066; Abcam), anti-SLC7A11 (PA1-16893; Invitrogen), anti-GAPDH (60004-1-Ig; Proteintech), goat anti-rabbit IgG H&L (HRP; ab205718; Abcam), HRP-conjugated affinipure goat anti-mouse IgG (H+L; SA00001-1; Proteintech), and goat anti-rabbit IgG H&L (Alexa Fluor 488; ab150077; Abcam) antibodies.

Chen Q., Wang L., Wei Y., Xu X., Guo X, & Liang Q. (2024). Ferroptosis as a Potential Therapeutic Target for Reducing Inflammation and Corneal Scarring in Bacterial Keratitis. Investigative Ophthalmology & Visual Science, 65(2), 29.

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Histological Analysis of Bone-Implant Interface

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Histological sections were generated parallel to the long axis of the implants around the undecalcified femoral condyle before decalcification. Sections were ground to 100 μm thickness, and slides were polished and stained with H&E and Goldner. Additionally, other bone samples without implants were fixed, decalcified, dehydrated, and embedded in paraffin. The tissues were cut into sections, being prepared for IHC staining (TNF-α, 1:200, Abcam ab1793) and 4-color immunofluorescence staining with the following primary antibodies (Abcam, UK): CD31 (1:500, ab182981), RUNX2 (1:200, ab236639), BMP-2 (1:200, ab214821), and DAPI (1:1000, ab285390) at 4 °C overnight. Finally, 4-color immunofluorescence staining was performed and visualized with Vectra Polaris (PerkinElmer, USA).

Huang Y., Wan X., Su Q., Zhao C., Cao J., Yue Y., Li S., Chen X., Yin J., Deng Y., Zhang X., Wu T., Zhou Z, & Wang D. (2024). Ultrasound-activated piezo-hot carriers trigger tandem catalysis coordinating cuproptosis-like bacterial death against implant infections. Nature Communications, 15, 1643.

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Immunohistochemical Analysis of Inflammatory and Repair Markers

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Immunohistochemistry was carried out using a two-step detection kit (Zhongshan Golden Bridge Biotechnology, China). Briefly, specimens were immersed in antigen retrieval solution for 20 min, blocked for 30 min with 5% bovine serum albumin (BSA), and subsequently incubated with primary antibodies against mouse TNF-α (Abcam, ab1793) and IL-6 (Abcam, ab290735) for inflammatory cytokines markers, CD31 (Abcam, ab182981) and TGF-β (Abcam, ab215715) for promoting repair markers at 1:100 dilution overnight at 4 °C. After rinsing thoroughly in PBS, the horseradish peroxidase-conjugated secondary antibodies (Zhongshan Golden Bridge Biotechnology) were dropped onto slides. Each group is composed of more than three slides, and each slide was observed using a Zeiss light microscopy at the defect area including the scaffolds (N > 3).

Peilin W., Ying P., Renyuan W., Zhuoxuan L., Zhenwu Y., Mai Z., Jianguo S., Hao Z., Gang Y., Lin L, & Haodong L. (2023). Size-dependent gold nanoparticles induce macrophage M2 polarization and promote intracellular clearance of Staphylococcus aureus to alleviate tissue infection. Materials Today Bio, 21, 100700.

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Immunofluorescence Imaging of Kidney Tissue

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For immunofluorescence (IF) staining, 4 mm sections of freshly frozen kidney tissue were fixed in 4% paraformaldehyde, washed in PBS for 30 min and incubated with the following primary monoclonal antibodies: Anti-TGFBI (1:200, ab170874, Abcam), Anti-TNF alpha (1:200, ab1793, Abcam), Anti-NF-kB p65 (phospho S536) (1:300, ab86299, Abcam), and Anti-oxLDL (1:300, ab14519, Abcam). The sections were washed with PBS, incubated with diluted fluorescence-conjugated secondary antibodies including donkey anti-rabbit IgG/FITC (Merck Millipore, Billerica, MA, USA) at 37°C in the dark for 1 h, and then stained with DAPI (Calbiochem). A micrograph of stained sections was acquired using a confocal microscope (Fluoview1000, Olympus, Tokyo, Japan) with FV10-ASW software (version 1.7, Olympus).

Zhang R., Wang T., Yin Q., Zhang J., Li L., Guo R., Han Q., Li H., Wang Y., Wang J., Gurung P., Lu Y., Cheng J., Bai L., Zhang J, & Liu F. (2019). FcgRIII Deficiency and FcgRIIb Defeciency Promote Renal Injury in Diabetic Mice. BioMed Research International, 2019, 3514574.

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Renal Tissue Histochemical and Immunohistochemical Analysis

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Renal tissues were fixed in 10% neutral buffered formaldehyde or 4% paraformaldehyde, embedded in paraffin and then 4 μm sections were cut and stained with standard periodic acid–Schiff, Masson's trichrome and histological techniques for light microscopic examination[16 (link), 17 (link)]. For immunohistochemical staining, primary antibodies against Anti-TGFBI (1:400, ab170874, Abcam), Anti-TNF alpha (1:400, ab1793, Abcam), Anti-NF-kB p65 (phospho S536) (1:600, ab86299, Abcam), and Anti-oxLDL (1:600, ab14519, Abcam) was used. The samples were then stained with ChemMate^TM Envision+HRP (Envision^TM Detection Kit, Code No: GK500705, Gene Tech, Shanghai) and incubated for 45 min at 37°C. Sections were then stained with diaminobenzidine (DAB) and counterstained with hematoxylin. In six mice per group, more than 20 cortical glomeruli were assessed for each mouse. The positive areas were quantified by Image-Pro Plus version 6.0 software according to methods previously reported [18 (link), 19 (link)].

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Immunofluorescence Staining of BV-2 Cells

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BV-2 cells were fixed in 2% paraformaldehyde (VVR Life Science) for 5 min, permeabilized by incubation in 0.05% Triton X-100 for 10 min, and blocked with 2.5% normal donkey serum for 1 h at room temperature (RT). The samples were then incubated with primary antibodies TNFa (1:100; AB1793, Abcam, Cambridge, MA, Unites States), COX-2 (1:100; MA5-14568, Thermo Fisher Scientific, Waltham, MA, Unites States), β-Tubulin (1:200; MA5-16308, Thermo Fisher Scientific, Waltham, MA, Unites States), or Alexa Fluor™ 647 Phalloidin conjugate (1:100; A22287, Thermo Fisher Scientific, Waltham, MA, Unites States) for 1 h at room temperature and washed with PBS-Tween 20 (0.05%; PBS-T). After PBS-T washing, they were then visualized by goat anti-rabbit IgG (H + L), Alexa Fluor 488 (A-11034, 1:1000, Fisher Scientific, Waltham, MA, Unites States), donkey anti-mouse IgG (H + L), Cyanine3 (A10521, 1:1000; Fisher Scientific, Waltham, MA, Unites States)/ The cell nuclei were visualized by incubation with 4′,6-diamidino-2-phenylindole (DAPI); (1:5000; Sigma). The slides were mounted with a ProLong Diamond antifade reagent (Thermo Fisher Scientific, Waltham, MA, Unites States).

Lennikov A., Yang M., Chang K., Pan L., Saddala M.S., Lee C., Ashok A., Cho K.S., Utheim T.P, & Chen D.F. (2022). Direct modulation of microglial function by electrical field. Frontiers in Cell and Developmental Biology, 10, 980775.

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Immunofluorescence Imaging of BMDM

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For IF, the BMDM were incubated with NF‐κB p65 (1:400, #8242S, CST), F4/80 (1:50, ab90247, abcam), TNFα (1:100, ab1793, abcam) and DAPI (1:3000, 2879038, Peprotech). Images were acquired using a confocal microscope (Zeiss LSM800).

Chen S., Lu Z., Zhao Y., Xia L., Liu C., Zuo S., Jin M., Jia H., Li S., Zhang S., Yang B., Wang Z., Li J., Wang F, & Yang C. (2024). Myeloid‐Mas Signaling Modulates Pathogenic Crosstalk among MYC+CD63+ Endothelial Cells, MMP12+ Macrophages, and Monocytes in Acetaminophen‐Induced Liver Injury. Advanced Science, 11(16), 2306066.

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Protein Expression Profiling Protocol

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Cells were lysed on ice for 30 min in lysis buffer containing 50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Nonidet P-40, and 0.1% SDS supplemented with protease inhibitors (10 mg/ml leupeptin, 10 mg/ml pepstatin A, and 10 mg/ml aprotinin). Protein fractions were collected by centrifugation at 10,000 × g for 10 min, separated on 10% SDS-PAGE gels, and then electrotransferred onto nitrocellulose membranes (Whatman, Piscataway, NJ, USA). The membranes were blocked with 5% BSA and then incubated with specific antibodies overnight at 4 °C. The primary antibodies and their sources were as follows: COL2A1 (1:1000, GB11021, Servicebio), ADAMTS4 (1:1000, ab185722, Abcam), p65 (1:5000, ab32536, Abcam), MMP3 (1:1000, ab52915, Abcam), MMP13 (1:1000, ab84594, Abcam), Caspase-7 (1:1000, DF6441, Affinity), cleaved Caspase-3 (1:1000, AF7022, Affinity), Caspase-3 (1:1000, AF6311, Affinity), Bcl-2 (1:2000, ab182858, Abcam), Bax (1:1000, ab32503, Abcam), TNF alpha (1:1000, ab1793, Abcam), β-actin (1:1000, ab6276, Abcam), and GAPDH (1:2000, AF7021, Affinity). HRP-conjugated secondary antibodies (ab205719 and ab6721, Abcam) were used at a 1:2000 dilution. The antigen–antibody complexes were visualized using an enhanced chemiluminescence detection system (Millipore, Darmstadt, Germany) according to the manufacturer’s instructions.

Zhu H., Hu Y., Wang C., Zhang X, & He D. (2020). CircGCN1L1 promotes synoviocyte proliferation and chondrocyte apoptosis by targeting miR-330-3p and TNF-α in TMJ osteoarthritis. Cell Death & Disease, 11(4), 284.

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