Cd25 pecy5

Chitosan (M_w 7 kDa, deacetylation degree > 95%) was purchased from Aoxing Bio-Technology Co. (Zhejiang, China). Folate was purchased from Bio Basic Inc. (Markham, ON, Canada). Monoclonal antibodies against mouse CD11b-FITC and Ly6G-PE were obtained from Beckman Coulter (Brea, CA, USA). Monoclonal antibodies against mouse CD4-FITC, CD8-PerCP-Cy5.5, CD25-PE-Cy5, CXCR3-FITC, Foxp3-PE and Foxp3 staining buffer were purchased from eBiosciences (San Diego, CA, USA). The anti-CD31 monoclonal antibody was obtained from Abcam (Cambridge, MA, USA). Purified hamster anti-mouse CD28 antibody was purchased from BD Biosciences (San Jose, CA, USA). The anti-mouse PCNA monoclonal antibody was supplied by Boster Bio-Technology Co. (Wuhan, China). TdT-mediated dUTP-biotin nick end labeling (TUNEL) cell-death fluorescein detection kit was obtained from Roche Diagnostics (Hoffmann-La Roche, Basel, Switzerland). Dynal mouse CD8⁺ T isolation kit was purchased from Invitrogen (Carlsbad, CA, USA). Collagenase I was acquired from Solarbio Co. (Beijing, China). The H-2K^b-restricted, melanoma-specific antigenic peptide TRP2 (amino acids 180 - 188, N-SVYDFFVWL-COOH) was chemically synthesized by Nanjing GenScript Corporation (Nanjing, China) with a purity > 95%.