All institutional and governmental regulations regarding the ethical use of were complied with during this study, which was approved by the Institutional Animal Care and Use Committee of Yeungnam University (YUMC-AEC2018-034). Male ICR mice weighing ∼28 g and C57BL6 mice weighing ∼20 g were purchased form Koatech (Gyeonggi, South Korea), ICR mice were used to analyze lipid profile and histochemistry in adipose tissue, and C57BL6 mice were used to determine circulating glucose disposal and molecular phenotype in skeletal muscle. Mice were housed under a 12:12-h light/dark cycle and provided a chow diet and water ad libitum. GW501516 (GW) was administered in diet at 40 mg/kg, and 5% EtOH was administered ad libitum for 5 weeks.
C57bl 6 mice
Manufactured by Koatech
Sourced in United States
C57BL/6 mice are a widely used inbred strain of laboratory mice. They are a common animal model used in biomedical research due to their well-characterized genetic background and physiological characteristics.
Automatically generated - may contain errors
Lab products found in correlation
Balb c mice, by Koatech (3 mentions)
High fat diet (hfd), by Research Diets (2 mentions)
Anti pecam 1 antibody, by BD (1 mentions)
Matrigel, by BD (1 mentions)
Sprague dawley rats, by Koatech (1 mentions)
Lyz2 cre mice, by Jackson ImmunoResearch (1 mentions)
Strain b6.129p2 p2ry2tm1bhk j, by Jackson ImmunoResearch (1 mentions)
Tissuelyser, by Qiagen (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Synaptophysin, by Merck Group (1 mentions)
Cryostat microtome, by Leica (1 mentions)
Goat anti doublecortin dcx antibody, by Santa Cruz Biotechnology (1 mentions)
Pc12 cell line, by Korean Cell Line Bank (1 mentions)
β actin, by Merck Group (1 mentions)
Balb c, by Koatech (1 mentions)
Rag2 γc mice, by Taconic Biosciences (1 mentions)
Rag1 mice, by Jackson ImmunoResearch (1 mentions)
Albumin from chicken egg white ova, by Merck Group (1 mentions)
Aluminum hydroxide, by Merck Group (1 mentions)
Research diet d12492, by Research Diets (1 mentions)
80 protocols using c57bl 6 mice
1
GW501516 Effects on Metabolic Phenotype
2
Colitis Induction and Therapeutic Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Colitis was induced in 7-week-old female C57BL/6 mice (Koatech, Pyeongtaek, Korea) by the addition of 1.5% (w/v) DSS (160110, MP Biomedicals, Santa Ana, CA, USA) to drinking water for 7 days. In total, 56 animals were divided into 7 groups (normal, colitis, 5-aminosalicylic acid (5-ASA), tofacitinib, and three doses of KM1608, n = 8). The normal group received drinking water without DSS, and the colitis-induced groups (colitis, 5-ASA, tofacitinib, and KM1608) received 1.5% DSS in drinking water. 5-ASA (200 mg/kg), tofacitinib (10 mg/kg), and KM1608 (50, 200, and 400 mg/kg) were orally administered once daily to colitis-induced mice for 7 days. 5-ASA and tofacitinib were used as reference drugs. For administration, 0.5% carboxymethylcellulose solution was used as a vehicle. The employed method for assessing the symptoms of colitis was derived from published literature and used with minor modifications [31 (link)]. On Day 7, the symptoms of colitis were assessed using the disease activity index (DAI) according to the following criteria: stool consistency—0, normal; 2, mild diarrhea; 4, severe diarrhea—and rectal bleeding: 0, normal; 2, mild bleeding; 4, severe bleeding. After CO2-induced euthanasia, the colon was collected to measure colon length and various inflammatory mediators.
3
Matrigel Plug Assay for Angiogenesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Seven-week-old C57BL/6 mice (Koatech, Pyeongtaek, Korea) were injected subcutaneously with 0.5 mL of Matrigel (BD Biosciences, San Jose, CA, USA) containing orexin-A, SnPP, and heparin (20 units). After 7 days, blood vessel infiltration level in Matrigel plugs was measured by hemoglobin analysis using the Drabkin method. To identify infiltrating endothelial cells, each Matrigel plug was stained with hematoxylin-eosin (H&E) and immunostained with anti-PECAM1 antibody (BD Biosciences, San Jose, CA, USA), a marker for endothelial cells.
4
Dietary Interventions in Mice Obesity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Three-week-old male C57BL/6 mice were purchased from KOATECH (Pyeongtaek, Korea) and maintained in the animal facility at Gyeongsang National University (GNU). Animal experiments were performed in accordance with the National Institutes of Health guidelines on the use of laboratory animals. The GNU Animal Care Committee for Animal Research approved the study protocol (GNU-130306-M0021). The mice were individually housed under a 12-h light/dark cycle. The mice had ad libitum access to food and water. To determine the effects of CR on changes in BAT induced by a HFD, mice were divided into two groups at 4 weeks of age and fed either a HFD (n = 20, 60 kcal% fat, 5.24 kcal/g; Research Diet, Inc., New Brunswick, NJ, USA) or normal standard diet chow (n = 10, normal diet [ND], 3.1 kcal/kg; Harlan Laboratories, Inc., Indianapolis, IN, USA) for 20 weeks. HFD-fed animals were then either continued on the HFD (n = 10) or subjected to CR (n = 10, 2 g/day of the HFD) for 12 weeks, as previously described [18 (link)].
5
Transgenic Mouse Modeling of Alzheimer's Disease
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All animal experimental procedures were approved by the Animal Care Committee of Seoul National University (Approval number: SNUIBC-080919-1). Transgenic mice with 5XFAD mutations were purchased from Jackson Laboratories (strain: B6SJL-Tg [APPSwFlLon, PS1*M146L*L286V] 6799Vas/J) and maintained by crossing hemizygous transgenic mice with B6SJL F1 mice. And wildtype male C57BL/6 mice (25–30 g) were supplied by Koatech (Pyeongtaek, Korea). Animal treatment and maintenance were performed in accordance with the Animal Care and Use Guidelines of Seoul National University, Seoul, Korea.
6
Sprague-Dawley Rat and C57BL/6 Mouse Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sprague-Dawley rats and C57BL/6 mice were purchased from Koatech Co. (Animal Breeding Center, Korea). Animal experiments were conducted in accordance with the guidelines of the National Institute of Health Guide for the Care and Use of Laboratory Animals (NIH Publication No. 80-23) and the Gyeongsang National University Animal Care and Use Committee (GLA-090805-R0065 and GLA-100310-M0024). Animals were housed under a 12-h light/dark cycle in pathogen-free conditions, with food and water freely available. All experiments were performed with the approval of the Research Ethics Committee of Gyeongsang National University.
7
Imiquimod-Induced Skin Inflammation Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All experiments were performed in accordance with the protocol and guidelines approved by the Institutional Animal Care and Use Committee of Seoul National University Hospital. Seven‐week‐old female C57BL/6 mice were purchased (Koatech, Korea). The mice were maintained in the semi‐SPF facility accredited by AAALAC International (#001 169) in accordance with Guide for the Care and Use of Laboratory Animals 8th edition, NRC (2010). After a week of acclimation, the mice were randomly grouped into five experimental groups (n = 5 in each group), and their backs were shaved and depilated, taking care not to damage the skin. Two days later (designated day 0), 5% imiquimod cream (62.5 mg for each mouse, Aldara cream, Dong‐A ST, Korea) was applied to the backs of the mice for six consecutive days to induce skin inflammation.[21 ] To quantify the severity of inflammation of the skin, erythema, scale, and lichenification were scored independently by two blinded evaluators (J.O. and B.M.K.) every day using a scale system from 0 to 4 (0, none; 1, slight; 2, moderate; 3, marked; 4, very marked) for each, resulting in a total score by adding up three items (range: 0–12).
8
Murine Models for Experimental Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Male ICR (6 weeks of age) and C57BL/6 mice (3 weeks of age) were purchased (KOATECH (Pyeongtaek, South Korea) and housed in the animal facility at Gyeongsang National University (GNU). Institutional Board of Research at GNU (GNU-160530-M0025) approved all animal experiments (24 October 2016). We performed in accordance with the National Institutes of Health guidelines for laboratory animal care. Mice were kept in a light/dark cycle of 12 h.
9
Thiacremonone Inhibits Lung Carcinoma in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
12-week-old C57BL/6J-Tg(Prdx6) mice were purchased from Jackson Lab (Maine, USA) and 12-week-old C57BL/6 mice were purchased from Koatech (Pyeongtaek, Korea). The mice were divided into four groups. Lewis Lung Carcinoma (LLC) cells were injected s.c. (1.2×106 tumor cells/0.1 mL PBS/animal) with a 27 gauge needle. After 10 days, two groups of mice (n = 10) were i.p. injected with thiacremonone (30 mg/kg in PBS and 0.01% DMSO) two times a week for 3 weeks. The control group of mice (n = 20) were treated with vehicle [PBS and 0.01% DMSO (i.p.)]) two times a week for 3 weeks. For subcutaneous tumors the maximum allowable size is 20 mm in diameter for a mouse, thus we sacrificed all mice before reaching maximum size Cervical dislocation was performed for euthanasia.
10
Acclimation and Care of Rodents and Primates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C57BL/6 mice were purchased from Koatech (Pyeongtaek, Korea) and maintained under a 12-hour dark/light cycle. Male and female drug-naïve cynomolgus monkeys were obtained from Covance Research Products (Alice, TX). All animals were acclimated to study conditions before dosing. Fresh food and water were provided daily, ad libitum. The care and treatment of all animals were in agreement with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research, and all procedures were performed in compliance with the Animal Welfare Act Regulations (9 CFR 3) and approved by the local Institutional Animal Care and Use Committee (18-626).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!