Goscript reverse transcriptase system
The GoScript Reverse Transcriptase System is a laboratory tool designed for the synthesis of complementary DNA (cDNA) from RNA templates. It provides a reliable and efficient means of converting RNA into cDNA for downstream applications such as gene expression analysis, PCR, and sequencing.
Lab products found in correlation
65 protocols using goscript reverse transcriptase system
Quantifying mRNA Levels with CGG Repeats
SAM68-dependent alternative splicing analysis of CGG expansions
Quantitative Real-Time PCR Protocol
Mosquito Dissection, RNA Extraction, and cDNA Synthesis
TRPV4 Channel Expression Analysis
TM Reverse Transcriptase System (Promega, Madison, USA) according to the manufacturer’s instructions. Semi-quantitative PCR was performed using a PCR kit (Tiangen, Beijing, China) on a thermo-cycler (Bio-Rad, Hercules, USA). PCR reactions were performed in a total volume of 25 μL containing 1 μL cDNA. Primers of the TRPV4 channel were designed for semi-quantitative PCR. All specific primers for the TRPV4 channel were designed based on Genbank mouse sequences. Specific primer sequences are as follows: TRPV4 channel, sense: 5′-TACGACCTGCTGCTTCTCAA-3′, antisense: 5′-TCCTCATCTGTCACCTCACG-3′; and GAPDH, sense: 5′-GGTGAAGGTCGGTGTGAACG-3′, antisense: 5′-CTCGCTCCTGGAAGATGGTG-3′. The cDNA product was PCR amplified using specific primers for the TRPV4 channel. The PCR annealing temperature for each primer pair was optimized using a Master Cycler Gradient Thermocycler (Eppendorf, Hamburg, Germany). PCR reaction consisted of 30 s denaturation at 94°C, 30 s for annealing at 58–64°C, 30 s for elongation at 72°C, and 30 amplification cycles. PCR products (15 μL) were separated by electrophoresis on a 1% agarose gel in Tris-borate/EDTA buffer. The quantification of semi-quantitative PCR was performed by using the optical density (OD) method.
Quantifying uhrf1 Expression in Wildtype and hmx1 Knockout Zebrafish
First-strand cDNA synthesis was performed using the AffinityScript™ Multiple Temperature Reverse Transcriptase kit (Agilent, Basel, Switzerland) according the manufacturer’s protocol. cDNA was generated (GoScript Reverse Transcriptase System; Promega) and Real time PCR (FastStart SYBR Green Master Roche) was performed using uhrf1-F
Gene expression change was determined using the 2–ΔΔCt method; relative values were normalized with β-actin.
Quantitative Real-Time PCR Verification
Quantitative Gene Expression Analysis
RNA Extraction and qPCR Analysis Protocol
Total RNA Extraction and qRT-PCR Analysis
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