Sodium citrate, Bovine Serum Albumin (BSA), DMSO, saponin, Capsaicin,
Resiniferatoxin and Staphylococcal Enterotoxin B (SEB) were from Sigma-Aldrich.
HDM extracts of the strain Dermatophagoides farinae were
purchased from Greer Laboratories. The following antibodies were obtained from
Covance: anti-Keratin (K) 14, anti-K6, anti-K10, anti-loricrin and
anti-filaggrin. Anti-Claudin 1 was from Abcam. Alexa594-conjugated goat
anti-rabbit IgG, Alexa488-conjugated avidin and DAPI were from Life Technologies
Invitrogen. The following reagent and antibodies were from eBioscience
Thermofisher Scientific: CellTrace™ CFSE Cell Proliferation Kit,
anti-CD4-APC, anti-IL4-PE, anti-IL-5-PE, anti-IL-13-PE, and anti-IFNγ-PE.
Purified NA/LE Rat Anti-Mouse CD124 (mIL4R-M1) or control Istoype (R35-95) were
from BD Bioscience. MYD88 inhibitory peptide (i.e., a cell permeant peptide
sequence that selectively blocks MYD88 homodimerization) and a control peptide
(i.e., an inactive and cell permeant truncated form of the MYD88 inhibitory
peptide) were from Novus. Alternaria alternata (A. alternata),
Dermatophagoides pteronyssinus (D. pteronyssinus), ragweed and
German Coackroach were all from Stallergenes Greer.
Resiniferatoxin and Staphylococcal Enterotoxin B (SEB) were from Sigma-Aldrich.
HDM extracts of the strain Dermatophagoides farinae were
purchased from Greer Laboratories. The following antibodies were obtained from
Covance: anti-Keratin (K) 14, anti-K6, anti-K10, anti-loricrin and
anti-filaggrin. Anti-Claudin 1 was from Abcam. Alexa594-conjugated goat
anti-rabbit IgG, Alexa488-conjugated avidin and DAPI were from Life Technologies
Invitrogen. The following reagent and antibodies were from eBioscience
Thermofisher Scientific: CellTrace™ CFSE Cell Proliferation Kit,
anti-CD4-APC, anti-IL4-PE, anti-IL-5-PE, anti-IL-13-PE, and anti-IFNγ-PE.
Purified NA/LE Rat Anti-Mouse CD124 (mIL4R-M1) or control Istoype (R35-95) were
from BD Bioscience. MYD88 inhibitory peptide (i.e., a cell permeant peptide
sequence that selectively blocks MYD88 homodimerization) and a control peptide
(i.e., an inactive and cell permeant truncated form of the MYD88 inhibitory
peptide) were from Novus. Alternaria alternata (A. alternata),
Dermatophagoides pteronyssinus (D. pteronyssinus), ragweed and
German Coackroach were all from Stallergenes Greer.