Supernatants, lysates, and pellet samples were separated by SDS‐PAGE using 10% or 16% polyacrylamide gels. After electrophoresis, the separated proteins were transferred to PVDF membranes (Pall Co., NY, USA). The membranes were probed overnight at 4°C with anti‐mouse IL‐1β antibody (R&D Systems, MN, USA) or anti‐actin antibody (Santa Cruz Biotechnology, CA, USA). The membranes were further probed with HRP‐conjugated secondary anti‐sera (Santa Cruz Biotechnology) and visualized by ECL solution (Millipore, MA, USA) and a cooled CCD camera system (ATTO Technology, Tokyo, Japan).
Anti mouse il 1β antibody
Manufactured by R&D Systems
Sourced in United States
The Anti-mouse IL-1β antibody is a research-use-only laboratory reagent. It is a highly specific antibody that binds to the mouse interleukin-1 beta (IL-1β) cytokine. This antibody can be used to detect and quantify IL-1β levels in mouse-derived samples.
Automatically generated - may contain errors
Lab products found in correlation
Adenosine triphosphate (atp), by Merck Group (7 mentions)
Nigericin, by Merck Group (5 mentions)
Lipopolysaccharides (lps), by Merck Group (4 mentions)
Glibenclamide, by Merck Group (3 mentions)
Anti mouse caspase 1, by Adipogen (3 mentions)
Mitosox, by Thermo Fisher Scientific (3 mentions)
Anti vdac1 antibody, by Abcam (3 mentions)
Staurosporine, by Merck Group (2 mentions)
Anti nlrp3, by Adipogen (2 mentions)
Poly da dt, by Merck Group (2 mentions)
Anti asc antibody, by Santa Cruz Biotechnology (2 mentions)
Mcc950, by Merck Group (2 mentions)
Anti β actin antibody, by Santa Cruz Biotechnology (2 mentions)
Anti actin antibody, by Santa Cruz Biotechnology (1 mentions)
Hrp conjugated secondary antisera, by Santa Cruz Biotechnology (1 mentions)
Ecl solution, by Merck Group (1 mentions)
Doxorubicin, by Merck Group (1 mentions)
Anti iκb antibodies, by Cell Signaling Technology (1 mentions)
Anti phospho jnk, by Thermo Fisher Scientific (1 mentions)
Anti phospho iκb, by Cell Signaling Technology (1 mentions)
9 protocols using anti mouse il 1β antibody
1
Western Blot Analysis of IL-1β
2
Inflammasome Activation and Inhibition
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Paclitaxel, doxorubicin, etoposide, LPS, ATP, nigericin, staurosporine, glibenclamide, and valiomycin were purchased from Sigma-Aldrich. Ciliobrevin D and SP600125 were purchased from Calbiochem. JC-1 and MitoSOX were purchased from Invitrogen. Alum was purchased from InvivoGen. Anti-mouse caspase-1, anti-NLRP3 and anti-ASC antibodies were obtained from AdipoGen. Anti-mouse IL-1β antibody was obtained from R&D Systems. Anti-phospho-IκB and anti- IκB antibodies were purchased from Cell Signaling Technology. Anti-phospho-JNK and anti-JNK antibodies were obtained from Invitrogen and BD, respectively. Anti-β-actin antibody was purchased from Santa Cruz Biotechnology.
3
Immunomodulatory Cytokine and Inflammasome Assays
4
NLRP3 Inflammasome Activation Assay
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LPS (L4391 for cell culture, L3012 for mice), ATP, MCC950, tamoxifen, and 4-hydroxytamoxifen were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-F4/80 antibody (Cat# ab6640, RRID:AB_1140040) was obtained from Abcam (Cambridge, United Kingdom). Cy3- (Cat# 712-165-050, RRID:AB_2340666) and Alexa Fluor 488-conjugated (Cat# 712-545-150, RRID:AB_2340683) anti-rat IgG antibodies were purchased from Jackson ImmunoResearch (West Grove, PA, USA). FITC-dextran (46944) and TRITC-dextran (T1037) were obtained from Sigma-Aldrich. The IL-1 receptor antagonist (Cat# CYT-203) was purchased from ProSpec (Rehovot, Israel). Anti-mouse caspase-1 (Cat# AG-20B-0042, RRID:AB_2490248) and anti-NLRP3 (Cat# AG-20B-0014, RRID:AB_2490202) antibodies were obtained from AdipoGen Life Sciences (San Diego, CA, USA). Anti-mouse IL-1β antibody (Cat# AF-401-NA, RRID:AB_416684) was obtained from R&D Systems (Minneapolis, MN, USA), and anti-ASC antibody (Cat# SC-22514-R, RRID:AB_2174874) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-GFP antibody (Cat# NB600-308, RRID:AB _10003058) was obtained from NOVUS Biologicals.
5
Inflammasome Activation Pathway Analysis
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FK866, lipopolysaccharide (LPS), nigericin, ATP, poly (dA:dT), poly (I:C) and nicotinamide mononucleotide (NMN) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Flagellin purified from P. aeruginosa was obtained from In vivoGen (San Diego, CA, USA). FK866 used in the in vivo experiments was purchased from Cayman (Ann arbor, MI, USA). Ciliobrevin D was obtained from Calbiochem (San Diego, CA, USA). Anti-mouse caspase-1 and anti-mouse NLRP3 antibodies were purchased from Adipogen (San Diego, CA, USA). Anti-apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) antibody was purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-mouse IL-1β antibody was obtained from R&D Systems (Minneapolis, MN, USA). Anti-mouse gasdermin D (GSDMD) and anti-VDAC1 antibodies were purchased from Abcam (Cambridge, MA, USA). Anti-mouse β-actin antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
6
Neuroinflammation and Dopamine Signaling
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MPTP was obtained from Sigma-Aldrich or Toronto Research Chemicals. MPP+, LPS, ATP, nigericin, staurosporine, propodium iodide, MCC950, dopamine and glibenclamide were purchased from Sigma-Aldrich. Mito-TEMPO was obtained from ENZO Life Sciences. MitoSOX was purchased from Invitrogen. Anti-mouse caspase-1 (AG-20B-0042) and anti-NLRP3 (AG-20B-0014) antibodies were obtained from Adipogen. Anti-mouse IL-1β antibody was obtained from R&D Systems (AF-401-NA). Anti-ASC antibody (SC-22514) and anti-phospho-ERK antibody (SC-7383) were purchased from Santa Cruz. Anti-CD45-BV421 antibody was obtained from BD Biosciences. Anti-ERK antibody (4695) and anti-phospho-JNK antibody (9251) were purchased from Cell Signaling. Anti-JNK antibody (554285) was obtained from BD pharmingen.
7
Inflammasome Activation in Murine Macrophages
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LPS, ATP, nigericin, cycloheximide (CHX), CCCP, mdivi-1, propidium iodide (PI), U0126, and Drp1-targeting or non-targeting shRNA lentiviral plasmids were purchased from Sigma. Alum was purchased from InvivoGen. Ac-YVAD-chloromethylketone (Ac-YVAD-cmk) and z-VAD-fluoromethylketone (zVAD-fmk) were obtained from Bachem. Mouse IL-1β enzyme-linked immunoassay (ELISA) kits were obtained from R&D. MitoSOX, MitoTracker Green, MitoTracker Deep Red, and mouse recombinant TNF-α were purchased from Invitrogen. Annexin V-FITC apoptosis detection kit was obtained from BD Bioscience. Anti-human caspase-1 (p10), anti-ASC, anti-phospho-ERK and anti-β-actin antibodies were purchased from Santa Cruz. Anti-mouse IL-1β antibody was obtained from R&D. Anti-mouse caspase-1 (p20) and anti-NLRP3 antibodies were from Adipogen. Anti-Drp1 and anti-JNK1/2 antibody were purchased from BD Biosciences. Anti-phospho-JNK1/2 antibody was obtained from Invitrogen. Anti-VDAC1 antibody was obtained from Abcam. All other antibodies detecting human IL-1β, caspase-3, ERK, and IκB were obtained from Cell Signaling.
8
Investigating Inflammatory Responses with Reagents
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RPMI 1640 and DMEM culture media, fetal bovine serum, glutamine, and a streptomycin/penicillin antibiotic solution were all purchased from Invitrogen (Paisley, UK). Bacterial LPS (Escherisha coli 026:B6), ZnPyr (zinc salt), TTM, TPEN, DTPA, pyrithione, CA, glyburide, and bafilomycin were purchased from Sigma (Gillingham, UK). Ac-YVAD-CHO, IETD-CHO, and Ca-074-Me were purchased from Merck Chemicals Ltd (Darmstadt, Germany). Salicylaldehyde isonicotinoyl hydrazone (SIH) was purchased from ChemBridge (San Diego, CA, USA). FluoZin-3 and Lysotracker Red were purchased from Invitrogen. The anti-mouse IL-1β antibody was from R&D Systems (Abingdon, UK). The XIAP and caspase-8 antibodies were purchased from Cell Signalling (Danvers, MA, USA), the actin antibody was from Sigma, and the caspase-1 antibody was from Santa Cruz Biotechnology (Dallas, TX, USA). Aβ peptide (1-42) was purchased from rPeptide (Bogart, GA, USA).
9
NLRP3 Inflammasome Activation Assay
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LPS, ATP, poly dA:dT, nigericin, and glibenclamide were purchased from Sigma-Aldrich (USA). Ultrapure LPS was obtained from Invivogen (USA). YVAD-chloromethylketone and z-VADfluoromethylketone were purchased from Bachem (Switzerland). Anti-mouse IL-1β antibody was obtained from R&D Systems (USA). Anti-mouse caspase-1 (p20) and anti-NLRP3 antibodies were from Adipogen (Switzerland). Anti-ASC antibody was obtained from Santa Cruz (USA).
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