Dulbecco s phosphate buffered saline dpbs ca2 mg2 free

Lithium heparin vacutainers were purchased from BD Biosciences (San Jose, CA). RPMI, fetal bovine serum (FBS), penicillin-streptomycin, Dulbecco’s phosphate-buffered saline (DPBS; Ca²⁺/Mg²⁺-free), Hank’s balanced salt solution (HBSS), MEGAclear™ Transcription Clean-Up Kit, Geneticin, Opti-MEM^®, and Lipofectamine^® RNAiMAX transfection reagent were purchased from Thermo Fisher Scientific (Waltham, MA). ODN2216 and chemically synthesized RNAs were synthesized by Integrated DNA Technologies, Inc. (IDT; Coralville, IA). A HiScribe™ T7 High Yield RNA Synthesis Kit, DNase I, and Antarctic Phosphatase were purchased from New England Biolabs (Ipswitch, MA). Multiplex chemiluminescence plates for the detection of type I IFNs were custom-manufactured by Quansys Biosciences (Logan, UT). Dulbecco’s Modified Eagle Medium (DMEM) was purchased from ATCC (Manassas, VA). QuickExtract™ DNA Extraction Solution was purchased from Epicentre (Madison, WI). KAPA HiFi HotStart DNA Polymerase was purchased from Kapa Biosystems (Wilmington, MA). A Mutation Discovery Kit for the Fragment Analyzer™ was purchased from Advanced Analytical Technologies, Inc. (Ames, IA).