Sd oct device

Spectral-domain (SD) OCT was performed using the SD-OCT device (Bioptigen, Inc., Durham. NC, USA) as described previously.^{23 (link)} Images were captured using the following parameters: rectangular scan: 1000 A-scans per B-scan, 100 B-scans per frame. Total retinal thicknesses were measured perpendicular to the surface of the RPE layer and 500 μm away from the center of the optic nerve at 12, 3, 6, and 9 o'clock direction with built-in software. All B-scan sections crossing the CNV were selected for analysis. CNV volume (μm³) was calculated with the following formula: Σ_n(a_n·t_n) (in which a_n is the area and t_n the thickness of the nth B-scan). The area of each B-scan passing through a CNV was calculated using ImageJ software (http://imagej.nih.gov/ij/; provided in the public domain by the National Institutes of Health, Bethesda, MD, USA).

Resolution of the retinal detachment induced by the subretinal cell delivery was monitored over time using a spectral‐domain optical coherence tomography (SD‐OCT) device (Bioptigen; Morrisville, NC, USA). Briefly, mice were anaesthetized and analysed using SD‐OCT before, immediately after, and at 5, 9 and 21 days post‐transplantation. Animals were excluded from the study if any complications affecting the opacity of the cornea or lens were observed. Retinal detachment was measured in SD‐OCT images using the area of maximum length between the retina and the retinal pigmented epithelium, using callipers provided directly by the software (InVivoVue Clinic 1.2 QOP‐60‐05 revision C). The measurements correspond to the area of larger detachment in each eye that, consequently, is the area of maximum delivery of the transplanted cells during the injections.