Retinol (Sigma-Aldrich), dissolved in ethanol (20 mg/mL), is mixed with an optimized PCL/PEG ratio.9 (link) A drug solution of equal parts of Retinol and PCL/PEG is made and loaded into the porous 3D printed TCP scaffold using 80 μL. Loading of 800 μg Retinol is employed because of the recommended dietary allowance between 700 and 900 μg of Retinol activity equivalents per day.19 Drug release is assessed at time points of 15, 30, and 45 min, 1.5, 3, 6, and 12 h, and 1, 3, and 7 days. Release buffers were analyzed using ultraviolet (UV)–visible spectroscopy (BioTek Synergy 2 SLFPTAD microplate reader). Retinol release assessed in pH 7.4 phosphate-buffered saline (PBS) and pH 5 acetate buffer solutions mimic physiological and microacidic environments after injury or surgery, respectively. A calibration curve is employed to quantify drug release concentration, and all release is monitored at 274 nm per manufacturing specifications (Sigma-Aldrich). Release plots are analyzed using Solver in Microsoft Excel and fitted for Weibull model.
Retinol
Manufactured by Merck Group
Sourced in United States, France, Germany, United Kingdom, China
Retinol is a type of vitamin A found in the Merck Group's lab equipment product line. It is a colorless or pale yellow, fat-soluble substance that plays a crucial role in various biological processes. Retinol is commonly used as a standard in laboratory applications that require the measurement or analysis of vitamin A levels.
Automatically generated - may contain errors
Lab products found in correlation
Retinyl palmitate, by Merck Group (8 mentions)
Retinyl acetate, by Merck Group (7 mentions)
Retinoic acid, by Merck Group (7 mentions)
α tocopherol, by Merck Group (7 mentions)
β carotene, by Merck Group (6 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (6 mentions)
Oleic acid, by Merck Group (5 mentions)
Cholesterol, by Merck Group (4 mentions)
Butylated hydroxytoluene bht, by Merck Group (4 mentions)
Methanol, by Merck Group (4 mentions)
Palmitic acid, by Merck Group (4 mentions)
Dauda, by Merck Group (3 mentions)
Tween 80, by Merck Group (3 mentions)
Methanol, by Thermo Fisher Scientific (3 mentions)
Lutein, by Merck Group (3 mentions)
Tetrahydrofuran, by Merck Group (3 mentions)
γ tocopherol, by Merck Group (3 mentions)
Chloroform, by Merck Group (3 mentions)
Dexamethasone (dex), by Merck Group (3 mentions)
Knockout serum replacement (ksr), by Thermo Fisher Scientific (3 mentions)
84 protocols using retinol
1
Retinol-Loaded 3D-Printed TCP Scaffold
2
Binding Affinities of FAR Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The binding activity of purified Ha- FAR-1, Ha- FAR-2 and Hf-FAR-1 proteins to fatty acid was measured using 11-((5-dimethylaminonaphthalene-1-sulfonyl) amino) undecanoic acid (DAUDA) (Sigma, USA) and retinol (Sigma, USA). In competition experiments, oleic acid (Sigma, USA) was used as a competitor of DAUDA or retinol. The monochromatic excitation wave lengths used for DAUDA and retinol were 345 nm and 350 nm, respectively. The equilibrium dissociation constant (Kd) was estimated by fluorescence titration [14 , 25 (link), 28 (link)]. The concentrations of protein solutions were gradually increased in 3 mL of PBS containing 3 μM DAUDA to determine the Kd of Ha-FAR-1 and Hf-FAR-1 binding to DAUDA, and containing 15 μM DAUDA for Ha-FAR-2 binding to DAUDA. The Kd for Ha-FAR-1, Ha-FAR-2 and Hf-FAR-1 binding to all-trans-retinol was estimated by adding increasing concentrations of all-trans-retinol to 5 μM protein in PBS.
3
Retinol and Cytokine Effects on Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The MODE-K cell line was from Kaiserlian and coworkers (29 (link)). HepG2 cells were from the ATCC. Cells were cultured in DMEM with GlutaMAX, 10% FBS, 1× Penstrep, and 1× sodium pyruvate. Cells were maintained in 5% CO2 at 37 °C. Before adding retinol, LPS, cytokines, or siRNAs, cells were cultured in serum-free medium for 48 h. MODE-K cells were treated for 24 h with retinol (100 nM; Sigma) and LPS (100 ng/mL; Sigma). HepG2 cells were treated for 24 h with retinol (100 nM), IL-1β (50 pg/mL; R&D Systems), and IL-6 (100 pg/mL; R&D Systems).
4
Sourcing Biochemical Reagents for Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ethanol, n-hexane, isopropanol, trichloromethane and HPLC grade dichloromethane, mEthanol, acetonitrile and water were purchased from Carlo Erba reagents (Val de Reuil, France). β-Carotene (HPLC purity > 96%) was from Carotenature GmbH (Münsingen, Switzerland). Retinol, retinyl palmitate, tocol, triolein, phospholipids, NaCl, sodium citrate, Tris hydroxide, bovine serum albumin and protease inhibitor cocktail were from Sigma Aldrich (Saint-Quentin Fallavier, France). Sevoflurane was from Baxter (Lublin, Poland), TRIzol reagent was from Euromedex (Souffelweyersheim, France). Dithiothreitol was from Thermo Fischer Scientific (Les Ulis, France). Phosphate buffered saline (PBS) was from life Technologies (Illkirch, France).
5
Retinoid Synthesis and Purification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Solvents, reagents and pure chemicals (retinyl palmitate and retinol) were obtained from Sigma-Aldrich (Saint Quentin Fallavier, France). 3,4-didehydroretinol was obtained from Santa Cruz biotechnology (Texas, United States) and polytetrafluoroethylene (PTFE) membranes from Sartorius (Palaiseau, France).
6
Lipid Composition Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
FA-free BSA, (S,S)-bis(monoacylglycero)phosphate (BMP), β-carotene, cholesterol, retinol (ROH), retinyl palmitate (RP), retinyl acetate, triolein, cholesteryl oleate, l -α-phosphatidylinositol, and 1,2-dioleoyl-sn-glycero-3-phosphocholine were purchased from Sigma. Lalistat 2 was a kind gift from Dr. Paul Helquist (Dept. of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN).
7
Standardized Dispersion Protocol for Oil Spills
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Retinol, retinaldehyde and retinoic acid were obtained from Sigma-Aldrich (St. Louis, MO) and were prepared as 1 mM stock in DMSO and stored in the vapor phase of nitrogen liquid. Corexit-EC9500A and Corexit-EC9527A (Nalco Energy Services, L.P., Sugar Land, TX) were kindly provided by Dr. Paddy Wiesenfeld at the U.S. FDA [41 (link)]. SPAN®80 (CAS#1338-43-8), TWEEN®80 (CAS#9005-65-6), TWEEN®85 (CAS#9005-70-3), Dioctyl sulfosuccinate sodium salt (DOSS, CAS#577-11-7), Di-(propylene glycol) butyl ether (CAS#29911-28-2), 2-Butoxyethanol (CAS#111-76-2), and 1,2-Propanediol (CAS#57-55-6) were purchased from Sigma-Aldrich. Chemical concentration of 1ppm is defined as 0.0001% v/v except for DOSS, which is 0.0001% w/v.
8
Stable Isotope Retinyl Acetate Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Whatman 903 protein saver cards (EU) and foil barrier resealable bags were purchased from Scientific Laboratory Supplies Ltd. The stable isotope retinyl acetate (8,9,10,11,12,13,14,15,19,20–13C10), of 98.8% all-trans isomeric purity and >99% 13C isotopic enrichment, was custom-synthesized by BuChem BV and conformed to Food Chemicals Codex 4th Edition testing. Acetic acid, formic acid, ethanol, methanol, and acetonitrile were purchased from Fisher Scientific and were certified for HPLC gradient analysis. Retinol and retinyl acetate were obtained from Sigma-Aldrich and were of >99% all-trans purity.
9
Generating Lipid Droplets in HEK293A Cells
10
Antarctic Krill Powder and Oil Extraction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antarctic krill (moisture content, 78.90 ± 0.75%) was supplied by Liaoyu Group Co., Ltd. (Dalian, China) and frozen at −23 °C. A part of them were used to prepare krill powder by a vacuum freeze dryer (Scientz-12N, Ningbo Science Biotechnology Co. Ltd, Ningbo, China) for 48 h and stored at −23 °C. The other part of them were used for extraction krill oil by SDE after thawed.
The DE (purity > 99.9%) was purchased from Hangzhou Airco Refrigerant Technology Co., Ltd. (Hangzhou, china). The standards of 37 fatty acid methyl esters (FAMEs), astaxanthin (≥97% HPLC),l -α-phosphatidylcholine (PC), l -α-phosphatidylethanolamine (PE), tocopherol and retinol were purchased from Sigma-Aldrich (Shanghai, China). All other reagents and solvents were provided by Fisher Scientific (Shanghai, China).
The DE (purity > 99.9%) was purchased from Hangzhou Airco Refrigerant Technology Co., Ltd. (Hangzhou, china). The standards of 37 fatty acid methyl esters (FAMEs), astaxanthin (≥97% HPLC),
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!