Leukocytic inflammatory foci in salivary glands were scored in a blinded fashion on H&E stained 5 μm sections of wax embedded salivary glands. 8 sequential sections of each gland with 40 μm intervals were scored by counting the number of focal infiltrates larger than 50 cells (16 (link)) and measuring the mean size of all foci per section. Foci size was measured with a Leica graticule with 10 ×10 mm grid and the sialitis score was calculated as:
where N̄f is the mean number of foci per section and S̄f is the average size of the foci per section.
Insulitis was scored on at least 100 islets per pancreas on H&E stained 5 μm sections with 40 μm interval and graded as follows: 1, no visible infiltrations, 2, periinsulitis; 3, insulitis with less than 50% islet infiltration; 4, insulitis with more than 50% islet infiltration. Percent of islets with insulitis (score 3 and 4) was used for the analyses.
where N̄f is the mean number of foci per section and S̄f is the average size of the foci per section.
Insulitis was scored on at least 100 islets per pancreas on H&E stained 5 μm sections with 40 μm interval and graded as follows: 1, no visible infiltrations, 2, periinsulitis; 3, insulitis with less than 50% islet infiltration; 4, insulitis with more than 50% islet infiltration. Percent of islets with insulitis (score 3 and 4) was used for the analyses.