Catalase assays

Manufactured by bioMérieux

Sourced in United States, France

The Catalase assays are a type of laboratory equipment designed to detect and measure the presence of the enzyme catalase in biological samples. Catalase is an important enzyme that helps break down hydrogen peroxide, a common byproduct of cellular metabolism. The assays provide a quantitative analysis of catalase activity, which can be useful in various research and diagnostic applications.

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Lab products found in correlation

Api 50ch, by bioMérieux (2 mentions) Dm1000 photonic microscope, by Leica (2 mentions) Api zym, by bioMérieux (2 mentions) Api 20ne, by bioMérieux (1 mentions) Color gram 2 kit, by bioMérieux (1 mentions) Morgani 268d, by Philips (1 mentions) Api 20a, by bioMérieux (1 mentions) Scanning electron microscope, by Hitachi (1 mentions) Genbag, by bioMérieux (1 mentions)

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Catalase (6 citations)

3 protocols using catalase assays

Characterization of Bacillus andreraoultii

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Bacillus andreraoultii strain SIT1^T was observed, after negative colouration, using a Morgani 268D (Philips, Amsterdam, The Netherlands) transmission electron microscope at an operating voltage of 60 kV. The Gram colouration was performed using Color Gram 2 Kit (bioMérieux) and observed by using the DM1000 photonic microscope (Leica Microsystems, Wetzlar, Germany) with a 100× oil-immersion objective lens. The sporulation test was done doing a thermic shock (80°C during 30 minutes). To evaluate the motility of Bacillus andreraoultii, fresh colonies were observed between blades and slats using a DM1000 photonic microscope (Leica) with a 40× objective lens.
API ZYM, API 20 NE and API 50 CH (bioMérieux) gallery systems were used to perform biochemical assays. Oxidase (Becton Dickinson, Franklin Lakes, NJ, USA) and catalase assays (bioMérieux) were done separately. The antibiotic susceptibility was tested using SirScan Discs antibiotics (i2a, Montpellier, France).

Traore S.I., Cimmino T., Lagier J.C., Khelaifia S., Brah S., Michelle C., Caputo A., Diallo B.A., Fournier P.E., Raoult D, & Rolain J.M. (2015). Noncontiguous finished genome sequence and description of Bacillus andreraoultii strain SIT1T sp. nov.. New Microbes and New Infections, 10, 25-35.

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Comprehensive Bacterial Characterization

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API Gallery systems were performed with API ZYM, API 20 A and API 50CH (bioMérieux) for biochemical assays. Oxidase (Becton Dickinson, Le Pont de Claix, France) and catalase assays (bioMérieux) were done separately. Sporulation was tested by performing thermal shock on bacterial colonies (diluted in phosphate-buffered saline) at 80°C for 10 minutes. The motility of strain GM3 was tested by observing its fresh colony between blades and slats using a DM1000 photonic microscope (Leica Microsystems, Nanterre, France) with a 40× objective lens.

Mourembou G., Rathored J., Ndjoyi-Mbiguino A., Lekana-Douki J.B., Fenollar F., Robert C., Fournier P.E., Raoult D, & Lagier J.C. (2015). Noncontiguous finished genome sequence and description of Gabonia massiliensis gen. nov., sp. nov.. New Microbes and New Infections, 9, 35-44.

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Characterization of Bacterial Strain C17T

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After 24 h of incubation, the bacterial cells were Gram-stained and observed using a Leica DM 500 photonic microscope (Leica Microsystems, Nanterre Cedex, France) with a 100 × oil immersion lens. Cell morphology was determined using a scanning electron microscope (Hitachi, Tokyo, Japan) set to the following conditions: accelerating voltage 30,000 V, magnification 7000, working distance 6700 μm, and emission current 112,000 nA. Cell motility was evaluated on soft agar plates (Xu et al. 2013 (link)). To determine the optimal culture conditions, several culture conditions were tested for strain C17^T. Culture assays were performed on Columbia agar supplemented with 5% defibrinated sheep blood (bioMerieux) at temperatures ranging from 4 to 45 °C (4 °C, 15 °C, 20 °C, 22 °C, 25 °C, 30 °C, 35 °C, 37 °C, 42 °C and 45 °C). The salt tolerance of strain C17^T was tested at various NaCl concentrations (1.5%, 2.0%, 2.5%, 3.0%, 3.5%, 4.5% and 6.5%). The oxygen demand was tested under aerobic, anaerobic, and microaerophilic (GENbag; BioMerieux) conditions. Different pH values (from 4.0 to 10.0) were also tested. Hemolytic activity was observed on Columbia blood agar plates. Catalase assays (bioMerieux) were performed following standard protocols. The oxidase reaction was assessed using the Becton Dickinson oxidase reagent (Becton Dickinson, Franklin Lakes, NJ, USA).

Qi H., Liu D., Zou Y., Wang N., Tian H, & Xiao C. (2021). Description and genomic characterization of Streptococcus symci sp. nov., isolated from a child’s oropharynx. Antonie Van Leeuwenhoek, 114(2), 113-127.

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