Originpro 8 sr0

The computations were carried out using OriginPro 8.SR0 and Excel software. The best fit sorption models were analyzed using the linear and nonlinear analysis, adjusted correlation coefficient (Adj. R²), and chi-square analysis (χ2) were computed.

We tested the hypotheses that diseased corals have more total vibrios (Hp1) and more V. coralliilyticus/V. harveyi (Hp2) than health corals by Welch's t-test with the GraphPad software (https://www.graphpad.com/). We examined the interaction between health state and sampling season by two-way ANOVA using the OriginPro 8 SR0 software (Origin Lab Corporation). We tested the correlation between the abundance of all vibrios and the abundance of V. coralliilyticus using Spearman's Rho Correlation (http://www.socscistatistics.com/tests/Default.aspx).

Statistical analyses were performed in OriginPro8 SR0 software package (v8.0724, OriginLab Corporation, Northampton, MA, USA), using one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test. The data are presented as mean ± SEM and considered statistically significant at p < 0.05.

All data on digestive enzyme activities, the abundances of genes and transcripts, and microbial α-diversity in the panda feces were calculated with OriginPro 8 SR0 (version 8.0724). Significant differences in α-diversity between groups were detected with Welch’s t-test. The R language vegan package was used to draw the OTU community bar charts and heatmaps. The Bray–Curtis method was used to calculate the distance between two samples and to quantify the differences in the species abundance distributions among samples. The OTU abundance table was standardized with Phylogenetic Investigation of Communities by Reconstruction of Unobserved States 2 (PICRUSt 2), a software package for the functional prediction of amplified 16S rRNA sequences, which removes the effect of the copy number of the 16S rRNA gene in the species genome. Each OTU corresponding to a phylogenetic lineage in this software was then used to annotate the Clusters of Orthologous Genes and KEGG functions of the OTUs to produce the OTU annotation information for each functional level and the abundance information for each function. PICRUSt 2 was also used to analyze the fungal data.

Chemical composition of microgels was examined by means of Fourier transform infrared spectroscopy coupled with attenuated total reflectance technique (ATR-FTIR). The spectra were acquired in the spectral region between 4000 and 400 cm⁻¹. The analysis was performed using the Origin software (OriginPro 8 SR0; OriginLab Corporation, Northampton, MA, USA).
To qualitatively detect the content of amino groups by gelatin content in the microgels, ninhydrin assay was also employed. A ninhydrin solution was prepared by dissolving 0.2 g of ninhydrin in 100 mL of ethanol. About 5 mg of microgels were placed in a tube with ninhydrin solution and heated at 80 °C for 15 min. The reaction of ninhydrin with amino groups induces a staining change from white to a deep purple color, as a function of the amount of amino groups.

All measurements were carried out three times in parallel. The experimental results were expressed by mean ± standard deviation (n = 3), and Origin Pro 8 SR0 (Origin Lab, Northampton, MA) was used to analyze the results by one-way ANOVA. The results were statistically significant when p < 0.05.

Wavelet analysis, correlation analysis, and fitting routines were performed using Matlab v7.6.0.324 (R2008a). Further statistics were performed using OriginPro 8 SR0 v8.0724 (B724).