The comparison studies were performed using 100 consecutive anonymized serum samples referred to the laboratory for routine testing, each of which was divided into two identical aliquots and simultaneously analyzed with both the Lumipulse G BRAHMS PCT and the ELFA immunoassay Vidas BRAHMS PCT (BioMérieux, Marcy l′Etoile, France). According to the manufacturer's data sheet, the measuring range of the latter method is between 0.05 and 200 ng/mL, and the inter- and intra-assay imprecision are between 1.9–4.6% and 3.6–7.0%, respectively. The correlation between the two methods was assessed with Passing and Bablok regression analysis and Spearman's correlation coefficient (r). The mean bias and its 95% Confidence Interval (95% CI) compared to Vidas BRAHMS PCT were estimated by Bland and Altman plot analysis. The agreement (and kappa statistics) of values was assessed at the three relevant diagnostic PCT thresholds of 0.5 ng/mL, 2.0 ng/mL and 10 ng/mL. A receiver operating characteristics (ROC) curve analysis was also used to define the agreement at the same PCT cut-offs.
Vidas brahms pct
Manufactured by bioMérieux
Sourced in France
The VIDAS BRAHMS PCT is a diagnostic instrument developed by bioMérieux. It is designed to measure procalcitonin (PCT) levels in patient samples. PCT is a biomarker that can help in the diagnosis and management of sepsis and other infectious conditions.
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Lab products found in correlation
Elisa kit, by Proteintech (1 mentions)
Liaison brahms pct 2 gen, by DiaSorin (1 mentions)
Immulite 2000, by Siemens (1 mentions)
Gem premier 3000, by Werfen (1 mentions)
Xe 2100, by Sysmex (1 mentions)
Tba 200fr, by Toshiba (1 mentions)
Cobas c702, by Roche (1 mentions)
Xe 2100 automated hematology system, by Sysmex (1 mentions)
12 protocols using vidas brahms pct
1
Analytical Evaluation of Procalcitonin Assays
2
Procalcitonin Quantification Protocol
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Levels of PCT were tested within 24 h after serum and fluid collection at room temperature (10–25°C) by enzyme linked fluorescent analysis (ELFA) using a PCT quantitative determination kit and fluorescence reading machine (Vidas® B.R.A.H.M.S PCT™; bioMérieux, Marcy l’Etoile, France) according to the manufacturers’ instructions.
3
Serum PCT Quantification by VIDAS
4
Biomarker Panel for ICU Admission
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A blood sample was obtained from patients within 6 hours (h) of admission to the ICU. Serum Gal-3 was measured by the human Gal-3 enzyme-linked immunosorbent assay (ELISA) Kit (BG Medicine, Corgenix, Inc., Broomfield, CO, USA; detection range: 1.4 ng/ml to 94.8 ng/ml), and serum creatinine (Cr) was measured using AU5831 Clinical Chemistry Analyzers (Beckman Coulter, Inc. Brea, CA, USA). In addition, serum neutrophil gelatinase-associated lipocalin (NGAL) and cystatin C (CysC) were measured with ELISA kits (Proteintech, Rosemont, IL, USA) according to the manufacturer`s instructions. Procalcitonin (PCT) was also measured using the ELFA (Enzyme-Linked Fluorescent Assay) technique by VIDAS® B•R•A•H•M•S PCT™ (bioMérieux, Inc., Marcy-l'Étoile, France).
5
Measuring Ct and ProCt in Multi-center Study
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All three institutions measured Ct using a two-site CLIA, (Immulite2000; Siemens Diagnostics, New Jersey, USA) with an analytical sensitivity of 2 ng/L. ProCt was measured at Institutions #1 and #3 using a two-site, two-step CLIA LIAISON BRAHMS PCT II GEN (DiaSorin, Saluggia, Italy), with an analytical sensitivity of 0.04 ng/ml, and at Institution #2 using an enzyme-linked fluorescence assay (ELFA) (BioMerieux VIDAS BRAHMS PCT, Hazelhood, USA) with an analytical sensitivity of 0.05 ng/ml.
6
Plasma Procalcitonin Measurement Protocol
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The VIDAS B-R-A-H-M-S PCT (bioMérieux, Inc., Durham, NC), an enzyme-linked fluorescent assay with a detection range of <0.05 ng/mL to >200 ng/mL, was used to measure plasma PCT. If insufficient sample volume was encountered, PCT was not recorded for that study day. If multiple samples were available, the earliest sample was used.
7
Plasma Biomarkers in Clinical Research
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Plasma samples had been stored at − 80 °C in EDTA tubes. Plasma IL-6 values were measured in nanograms per liter using an enzyme-linked immunosorbent assay (ELISA Elecsys IL-6) assay. Plasma PCT levels were determined by an enzyme-linked fluorescent immunoassay (VIDAS® B.R.A.H.M.S. PCT™, provided by bioMérieux) and displayed in nanograms per milliliter. The lower detection limit was 0.05 ng/mL. Cutoff values were based on our previous research and literature findings and set at 60 ng/L and 0.25 ng/mL for IL-6 and PCT, respectively [6 (link), 14 (link), 15 (link)].
8
Procalcitonin and Gut Microbiome Analyses
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Within the framework of this study, we also piloted two mechanistic sub-studies with plans for expansion within the larger RCT. First, in a procalcitonin (PCT) sub-study, we obtained blood PCT measurements on the day of randomization, and days 7, 10, and 14 from the index blood culture collection. The results were not made available to the treating team because this could have unduly influenced protocol adherence; rather, they were batch-tested at the end of the trial using VIDAS® B.R.A.H.M.S. PCT™, BioMérieux (Marcy L’Etoile, France). The outcome of interest was the proportion of patients for whom PCT levels exceeded the usual threshold (0.25 IU/mL) for recommending antibiotic treatment at day 7 and day 14. At a single ICU site, we also collected rectal swabs on the day of enrollment, day 7, day 14, and at either hospital discharge or 28 days post enrollment, for analysis of gut microbial diversity and bacterial community composition by 16S rRNA gene sequencing, with the main outcome being taxonomic diversity (Shannon Diversity Index) [22 (link)].
9
Comprehensive Biomarker Assessment Protocol
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For each sample, LA, PCT, CRP, IPF, IG, IRF, white blood cell (WBC) counts, hemoglobin, platelets, WBC differential counts, prothrombin time (PT), activated partial thromboplastin time (aPTT), D-dimer, creatinine, glucose, and total bilirubin were measured. The LA level was measured by using a GEM Premier 3000 instrument (Instrumentation Laboratory, Lexington, MA, USA). The PCT level was measured by immunoluminometric assay (VIDAS B.R.A.H.M.S. PCT; bioMerieux, Saint Laurent, Canada), and the CRP level was measured by latex immunoturbidimetric assay (Tina-quant CRP HS Test System; Roche, Basel, Switzerland). All hematological and coagulation parameters were obtained from an automated blood cell analyzer (Sysmex XE-2100; Sysmex, Kobe, Japan) and coagulation analyzer (Sysmex CA-7000; Syxmex). The creatinine, glucose, and total bilirubin levels were measured by using a clinical chemistry analyzer (TBA-200FR; Toshiba Medical Systems, Tokyo, Japan). This study was approved by the institutional review board of each author's institution.
10
Serum CRP and PCT Quantification
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Serum CRP concentrations were quantified using a cobas c 702 automated clinical chemistry analyzer (Roche, Basel, Switzerland). The assay was based on immunoturbidimetry, with the detection range of 0.03–350 mg/dL. Serum PCT concentrations were measured using VIDAS® B.R.A.H.M.S PCT™ (bioMérieux), with the detection range of 0.05–200 ng/mL. The patients with CRP or PCR values below the detection limit were excluded. In addition, the patients who did not receive the PCT testing were excluded. Of the 440 participants included in this study, 227 patients had data on the CRP and PCT concentrations at the BC sampling time.
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