1010 microscope

Manufactured by JEOL

Sourced in United States, Japan

The JEOL 1010 is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of a wide range of samples. The instrument features a high-resolution objective lens and advanced electron optics to provide clear, detailed images of specimens at the nanometer scale. The JEOL 1010 is capable of performing various analytical techniques, including selected-area electron diffraction and energy-dispersive X-ray spectroscopy, to characterize the structural and elemental properties of samples.

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Lab products found in correlation

Fcf 200 cu, by Electron Microscopy Sciences (3 mentions) 2010f tem stem, by JEOL (1 mentions) Tem 2100, by JEOL (1 mentions) Spurrs resin, by Agar Scientific (1 mentions) Jsm 7001f field emission sem, by JEOL (1 mentions) Eagle ccd camera, by Thermo Fisher Scientific (1 mentions) Tecnai f20, by Thermo Fisher Scientific (1 mentions) Tecnai t12 microscope, by Thermo Fisher Scientific (1 mentions) Icp oes, by SPECTRO Analytical Instruments (1 mentions) Zetasizer nano zs90, by Malvern Panalytical (1 mentions) Bacteriological agar, by Thermo Fisher Scientific (1 mentions) Osmium tetroxide, by TAAB (1 mentions) Epon 812, by Tousimis (1 mentions) Uv 2101pc spectrophotometer, by Shimadzu (1 mentions) Tripletoft5600, by AB Sciex (1 mentions) Varian cary eclipse, by Agilent Technologies (1 mentions) Silica gel 60 f254, by Merck Group (1 mentions) 300 mhz spectrometer, by Bruker (1 mentions) Auto 306, by Edwards Lifesciences (1 mentions)

17 protocols using 1010 microscope

Transmission Electron Microscopy of BION Formulations

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Transmission electron microscopy of each BION formulation was performed using a JEOL 1010 microscope operating at 80 kV. Briefly, 10 μl of diluted BION (10 μl from stock was diluted with 1 ml of DI water) was dropped onto carbon coated copper grid (FCF-200-Cu, Electron Microscopy Sciences, Hatfield, PA, USA), and allowed to evaporate before imaging. High-resolution images and SAED patterns were collected at 200 kV on a JEOL 2010F TEM/STEM and a JEOL 2100 TEM, respectively. Samples were mounted from suspension onto 200 mesh Cu grids with lacy carbon films.

Naha P.C., Zaki A.A., Hecht E., Chorny M., Chhour P., Blankemeyer E., Yates D.M., Witschey W.R., Litt H.I., Tsourkas A, & Cormode D.P. (2014). Dextran coated bismuth-iron oxide nanohybrid contrast agents for computed tomography and magnetic resonance imaging. Journal of materials chemistry. B, Materials for biology and medicine, 2(46), 8239-8248.

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Ultrastructural Analysis of Apoptotic β-cells

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Isolated islets were fixed in 4% paraformaldehyde and 0.5% glutaraldehyde in phosphate buffer for 1 h, post-fixed in 1% osmium tetroxide, block stained in 2% uranyl actetate, dehydrated in graded ethanol and embedded in Spurr’s resin (Agar Scientific, Stansted, UK). Ultrathin sections (70 nm) were cut onto Ni²⁺ grids, contrasted with 2% uranyl acetate and lead citrate, and examined in a Jeol 1010 microscope (Welwyn Garden City, UK) with an accelerating voltage of 80 kV.
β-Cell apoptosis was assessed from electron micrographs as the number of β-cells with apoptotic nuclei (that is, displaying typical chromatin condensation): β-cells (135–150 from n=3–9 mice; n=3 islets per mouse) were identified by the presence of characteristic insulin granules.

Brereton M.F., Iberl M., Shimomura K., Zhang Q., Adriaenssens A.E., Proks P., Spiliotis I.I., Dace W., Mattis K.K., Ramracheya R., Gribble F.M., Reimann F., Clark A., Rorsman P, & Ashcroft F.M. (2014). Reversible changes in pancreatic islet structure and function produced by elevated blood glucose. Nature Communications, 5, 4639.

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Ultrastructural Analysis of Rat Vestibular Epithelia

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For SEM and TEM, rat vestibular epithelia were dissected in 2.5% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.2), and fixed overnight in this solution. The epithelia were then rinsed with cacodylate buffer, post-fixed for 1 h in 1% osmium tetroxide in the same buffer, rinsed again and stored in 70% ethanol at 4 °C until further processing. Afterwards, the samples were dehydrated with increasing concentrations of ethanol, up to 100%. For TEM analysis, the lateral crista was embedded in Spurr resin. To select the level of interest, semi-thin sections (1 µm) were stained with 1% toluidine blue and examined in a light microscope. Ultrathin sections were stained with uranyl acetate and lead citrate and were observed with a JEOL 1010 microscope at 75–80 kV. For SEM analysis of epithelial surfaces, the dehydrated specimens were dried in a Polaron E3000 critical-point dryer using liquid CO2, coated with carbon, and observed in a JEOL JSM-7001F field emission SEM.

Maroto A.F., Borrajo M., Prades S., Callejo À., Amilibia E., Pérez-Grau M., Roca-Ribas F., Castellanos E., Barrallo-Gimeno A, & Llorens J. (2023). The vestibular calyceal junction is dismantled following subchronic streptomycin in rats and sensory epithelium stress in humans. Archives of Toxicology, 97(7), 1943-1961.

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Electron Microscopy of Dex-CeNP

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Electron microscopy (EM) of Dex-CeNP was performed using a JEOL 1010 microscope operating at 80 kV. Diluted Dex-CeNP was dropped onto the EM grid (FCF-200-Cu, Electron Microscopy Sciences, Hatfield, PA) and the liquid was allowed to dry before microscopy was performed. High-resolution electron micrographs were collected using a JEOL 2010F microscope operating at 200 kV from Dex-CeNP mounted onto 200 mesh Cu grids with lacy carbon films.

Naha P.C., Hsu J.C., Kim J., Shah S., Bouché M., Si-Mohamed S., Rosario-Berrios D.N., Douek P., Hajfathalian M., Yasini P., Singh S., Rosen M.A., Morgan M.A, & Cormode D.P. (2020). Dextran Coated Cerium Oxide Nanoparticles: A Computed Tomography Contrast Agent for Imaging the Gastrointestinal Tract and Inflammatory Bowel Disease. ACS nano, 14(8), 10187-10197.

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Exosome Characterization by TEM

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Human and bovine exosomal fractions (fractions 7–10) were pooled, and 5 μl from each sample was analysed by transmission electron microscopy (TEM) by negative stain. Samples were placed on formvar-coated copper grids and viewed on a JEOL 1010 microscope [1 (link)].

Vaswani K., Mitchell M.D., Holland O.J., Qin Koh Y., Hill R.J., Harb T., Davies P.S, & Peiris H. (2019). A Method for the Isolation of Exosomes from Human and Bovine Milk. Journal of Nutrition and Metabolism, 2019, 5764740.

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Transmission Electron Microscopy of Samples

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The size and morphology
of the samples was studied by transmission
electron microscopy (TEM) using a JEOL 1010 microscope operating at
100 kV. For that purpose, the samples were suspended in 2-propanol
and deposited onto copper grids.

García-Ben J., López-Beceiro J., Artiaga R., Salgado-Beceiro J., Delgado-Ferreiro I., Kolen’ko Y.V., Castro-García S., Señarís-Rodríguez M.A., Sánchez-Andújar M, & Bermúdez-García J.M. (2022). Discovery of Colossal Breathing-Caloric Effect under Low Applied Pressure in the Hybrid Organic–Inorganic MIL-53(Al) Material. Chemistry of Materials, 34(7), 3323-3332.

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Characterization of Multilamellar Vesicles

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The hydrodynamic diameter and the corresponding polydispersity index (PI) of MLs were determined by dynamic light scattering at a fixed scattering angle of 90° with a Zetasizer Nano (Malvern, UK) at 25 °C. MLs from the stock solution were dispersed in water to obtain approximately 0.1 g·L⁻¹ solid content. Geometry of MLs was observed by transmission electron microscopy (TEM) and cryo-TEM. For TEM observations, a Jeol 1010 microscope (Jeol, Tokyo, Japan) operating at 80,000 kV was used. Samples were prepared by placing a drop of MLs onto a 400-mesh copper grid coated with carbon, and after staining with uranyl acetate they were allowed to dry in the air before placing into the microscope. Images were recorded with a Megaview camera. Acquisition was accomplished with the Soft-Imaging software (SIS, Münster, Germany). For cryo-TEM observations, grids were transferred to a Tecnai F20 (FEI, Eindhoven, The Netherlands) using a cryoholder (Gatan, Warrendale, PA, USA). Images were taken at 200 kV, at a temperature ranging from −175 to −170 °C and using low-dose imaging conditions with a 4096 × 4096 pixel CCD Eagle camera (FEI, Eindhoven, The Netherlands).

Martínez-González R., Estelrich J, & Busquets M.A. (2016). Liposomes Loaded with Hydrophobic Iron Oxide Nanoparticles: Suitable T2 Contrast Agents for MRI. International Journal of Molecular Sciences, 17(8), 1209.

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Retinal Ultrastructure Analysis by TEM

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Retinal morphology was examined using transmission electron microscopy (TEM). Eyeballs were removed and processed as described above, and cut into ultrathin (75 nm) sections. They were then stained with Reynold’s lead citrate and washed in distilled water. The images were captured on a Jeol 1010 microscope (JEOL, Peabody, MA, USA) at 80 kV using DigitalMicrograph software version 3 (Gatan, Abingdon, UK).

Lee V.K., Hosking B.M., Holeniewska J., Kubala E.C., Lundh von Leithner P., Gardner P.J., Foxton R.H, & Shima D.T. (2018). BTBR ob/ob mouse model of type 2 diabetes exhibits early loss of retinal function and retinal inflammation followed by late vascular changes. Diabetologia, 61(11), 2422-2432.

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Nanoparticle Characterization via TEM

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Transmission electron microscopy (TEM) was used to determine the core sizes and morphologies of the core metal nanoparticles and PCPP nanoparticles. The images were acquired using a Tecnai T12 microscope (FEI, Hillsboro, OR, USA) or a JEOL 1010 microscope (JEOL Ltd., Tokyo, Japan). Diameters of 500 individual nanoparticles of each formulation were manually measured on TEM images using ImageJ. The hydrodynamic diameter and zeta potential of the nanoparticles were assessed by using a Nano-ZS-90 Zetasizer (Malvern Instruments, Worcestershire, UK) by preparing the samples at 0.2 mg/ml in concentration. The concentrations of the elemental payloads in the nanoparticles were measured by using ICP-OES (Spectro Analytical Instruments GmbH, Kleve, Germany).

Kim J., Silva A.B., Hsu J.C., Maidment P.S., Shapira N., Noël P.B, & Cormode D.P. (2019). Radioprotective garment-inspired biodegradable polymetal nanoparticles for enhanced CT contrast production. Chemistry of materials : a publication of the American Chemical Society, 32(1), 381-391.

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T cell expansion and ultrastructural imaging

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T cells were logarithmically expanded in the presence of either vehicle or 50uM ETO for several days and subsequently frozen at restdown. Upon thaw, the cells were prepared with Penn’s Electron Microscopy Resource Laboratory and imaged with the Jeol-1010 microscope.

O’Connor R.S., Guo L., Ghassemi S., Snyder N.W., Worth A.J., Weng L., Kam Y., Philipson B., Trefely S., Nunez-Cruz S., Blair I.A., June C.H, & Milone M.C. (2018). The CPT1a inhibitor, etomoxir induces severe oxidative stress at commonly used concentrations. Scientific Reports, 8, 6289.

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