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Peroxidase conjugated anti mouse and anti rabbit iggs

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Peroxidase-conjugated anti-mouse and anti-rabbit IgGs are secondary antibodies that are conjugated with horseradish peroxidase (HRP). They are used to detect and quantify the presence of mouse or rabbit primary antibodies in immunoassays and immunoblotting applications.

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Lab products found in correlation

Ecl prime western blotting detection reagent, by Cytiva (3 mentions) Chemidoc mp imager, by Bio-Rad (3 mentions) Image lab, by Bio-Rad (3 mentions) Bca reagent, by Thermo Fisher Scientific (2 mentions) Ndufs1, by GeneTex (1 mentions) Turbo gfp, by OriGene (1 mentions) Cox5b, by Santa Cruz Biotechnology (1 mentions) β actin, by Merck Group (1 mentions) Ndufb8, by Abcam (1 mentions) Core 2, by Abcam (1 mentions) Cox5a, by Abcam (1 mentions)

Close spelling variants of this product

Peroxidase-conjugated anti-rabbit IgG Peroxidase-conjugated anti-mouse IgG Mouse and rabbit IgG Rabbit anti-mouse IgG Mouse anti-IgG IgG Rabbit

4 protocols using peroxidase conjugated anti mouse and anti rabbit iggs

1

Quantitative Western Blot Analysis

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Protein concentration was measured with the BCA reagent (Thermo Scientific). 20–60 mg of mitochondrial protein extract was separated by SDS–PAGE in the Laemmli buffer system (Laemmli, 1970 (link)). Then, proteins were transferred to nitrocellulose membranes at 40 V overnight and probed with specific primary antibodies listed in the Key reagents Table. Peroxidase-conjugated anti-mouse and anti-rabbit IgGs were used as secondary antibodies (Molecular Probes). Immunoreactive bands were detected with an ECL prime Western Blotting Detection Reagent (Amersham) in a ChemiDoc MP Imager (Biorad) or by exposition to X-ray films. Optical densities of the immunoreactive bands were measured using the ImageLab (Biorad) software or the ImageJ software in digitalized images.
Lobo-Jarne T., Nývltová E., Pérez-Pérez R., Timón-Gómez A., Molinié T., Choi A., Mourier A., Fontanesi F., Ugalde C, & Barrientos A. (2018). Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics. Cell reports, 25(7), 1786-1799.e4.
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2

Comprehensive Western Blot Analysis

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Western blot was performed using primary antibodies raised against COX7A2L (ProteinTech), Myc (Origene), turbo-GFP (Origene), HA (Roche), β-actin (Sigma), and against the following human OXPHOS subunits: NDUFS1 (GeneTex); NDUFA9, NDUFB8, CORE2, RISP, CYC1, UQCRB, UQCRQ, COX1, COX4, COX5A, COX6C, SDHA, SDHB (Mitosciences); and COX5B (Santa Cruz). Peroxidase-conjugated anti-mouse and anti-rabbit IgGs were used as secondary antibodies (Molecular Probes). Immunoreactive bands were detected with an ECL prime Western Blotting Detection Reagent (Amersham) in a ChemiDoc™ MP Imager (Biorad). Optical densities of the immunoreactive bands were measured using the ImageLab™ (Biorad) and ImageJ analysis softwares.
Pérez-Pérez R., Lobo-Jarne T., Milenkovic D., Mourier A., Bratic A., García-Bartolomé A., Fernández-Vizarra E., Cadenas S., Delmiro A., García-Consuegra I., Arenas J., Martín M.A., Larsson N.G, & Ugalde C. (2016). COX7A2L is a mitochondrial complex III-binding protein that stabilizes the III2+IV supercomplex without affecting respirasome formation. Cell reports, 16(9), 2387-2398.
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3

Mitochondrial Protein Quantification and Analysis

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Protein concentration was measured by the Lowry method (Lowry et al., 1951) . 40-80 mg of mitochondrial protein extract was separated by SDS-PAGE in the Laemmli buffer system (Laemmli, 1970) . Then, proteins were transferred to nitrocellulose membranes and probed with specific primary antibodies listed in Key Resources Table . Peroxidase-conjugated anti-mouse and anti-rabbit IgGs were used as secondary antibodies (Molecular Probes). Optical densities of the immunoreactive bands were measured using the ImageJ software in digitalized images.
Timón-Gómez A., Garlich J., Stuart R.A., Ugalde C, & Barrientos A. (2020). Distinct Roles of Mitochondrial HIGD1A and HIGD2A in Respiratory Complex and Supercomplex Biogenesis. Cell reports, 31(5).
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4

Quantitative Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein concentration was measured with the BCA reagent (Thermo Scientific). 20–60 mg of mitochondrial protein extract was separated by SDS–PAGE in the Laemmli buffer system (Laemmli, 1970 (link)). Then, proteins were transferred to nitrocellulose membranes at 40 V overnight and probed with specific primary antibodies listed in the Key reagents Table. Peroxidase-conjugated anti-mouse and anti-rabbit IgGs were used as secondary antibodies (Molecular Probes). Immunoreactive bands were detected with an ECL prime Western Blotting Detection Reagent (Amersham) in a ChemiDoc MP Imager (Biorad) or by exposition to X-ray films. Optical densities of the immunoreactive bands were measured using the ImageLab (Biorad) software or the ImageJ software in digitalized images.
Lobo-Jarne T., Nývltová E., Pérez-Pérez R., Timón-Gómez A., Molinié T., Choi A., Mourier A., Fontanesi F., Ugalde C, & Barrientos A. (2018). Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics. Cell reports, 25(7), 1786-1799.e4.
+ Open protocol
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