Q exactive plus quadrupole orbitrap

High-resolution mass spectrometry detection was performed on both Exactive Plus Orbitrap and Q-Exactive Plus Quadrupole-Orbitrap mass spectrometers (Thermo) equipped with an electrospray ionization source and operated in positive mode. Screening and confirmation of the substances were performed using full scan mode in Exactive Plus and parallel reaction monitoring (PRM) mode in Q-Exactive Plus. For identification of selected molecules, the scan range and collision energy were applied to each substance at a resolution of 35,000 in PRM mode.

Method development and optimization for analysis of oxidized complex lipids were performed using RP-UHPLC coupled on-line either to a Thermo Scientific Q Exactive Plus Quadrupole-Orbitrap (Thermo Fisher Scientific, Bremen, Germany) or Orbitrap Fusion Lumos Tribrid (Thermo Fisher Scientific, San Jose, USA) mass spectrometers.
Q Exactive Plus Quadrupole-Orbitrap was equipped with a HESI source and operated in both positive and negative ion modes with the following parameters: sheath gas—40 arb.units, auxiliary gas—10 arb.units, sweep gas—1 arb.units, spray voltage—+3.5 kV and −2.5 kV, capillary temperature—300 °C, S-lens RF level—35%, and aux gas heater temperature—370 °C.
Orbitrap Fusion Lumos Tribrid mass spectrometer using a HESI source was operated in both positive and negative ion modes with the following parameters: spray voltage—+3.5 kV and −2.8 kV, ion transfer tube temperature—250 °C, sheath gas—25 arb.units, aux gas—10 arb.units, vaporizer temperature—200 °C, RF level—25%.