Ds fi1 compound microscope

Specimens were borrowed from Queensland Plant Pathology Herbarium (BRIP) and Herbarium Mycologicum Academiae Sinicae (HMAS) (Table 1). Spores were mounted in lactic acid (100% v/v) and examined under the light microscope. Means and standard deviations (SD) were calculated from at least 20 measurements. Ranges were expressed as (min.−) mean − SD–mean + SD (−max.) with values rounded to 0.5 μm if below 20 μm and 1.0 μm if above 20 μm. Images were captured by using a Nikon Eclipse 80i camera attached to a Nikon DS-Fi1 compound microscope with Nomarski differential interference contrast. For scanning electron microscopy (SEM), dried spores were dusted onto double-sided adhesive tape, fixed on specimen stubs, sputter coated with gold, ca. 20 nm thick, and examined with a FEI Quanta 200 electron microscope. Nomenclatural novelties and descriptions were registered in MycoBank (www.MycoBank.org).

Semi-thin (80–100 μm) young leaf and stem sections were viewed at various wavelengths with a Nikon DS-Fi1 compound microscope (Nikon, Japan) equipped with NIS Elements D software. The sections were stained with Acridine orange and 0.01% Calcofluor white solution to detect the viability of cells and cellulose in cell walls, respectively [52 (link)].