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Seeplex rv15 onestep ace detection

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The Seeplex RV15 OneStep ACE Detection is a multiplex real-time PCR assay designed for the simultaneous detection of 15 respiratory viruses, including influenza A/B, respiratory syncytial virus, and others. The product utilizes Seegene's proprietary ACE technology to enable efficient and accurate detection of target pathogens in a single reaction.

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Lab products found in correlation

Geneamp pcr system 9700, by Thermo Fisher Scientific (1 mentions) Mce 202 multina device, by Shimadzu (1 mentions)

Spelling variants of this product

Seeplex® RV15 OneStep ACE Detection kit (4 citations) Seeplex RV15 ACE Detection (2 citations) RV15 ACE Detection (2 citations)

4 protocols using seeplex rv15 onestep ace detection

1

Multiplex Viral Detection by RT-PCR

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Viral DNA and RNA were extracted using Ribo_spin vRD (GeneAll, Seuol, Korea) in accordance with the manufacturer’s instructions. The isolated DNA and RNA were amplified using the one-step RT-PCR of Seeplex RV15 OneStep ACE detection (Seegene Inc., Seoul, Korea) which can detect the following 15 respiratory viruses; parainfluenza virus 1/2/3/4, adenovirus, coronavirus 229E/NL63/OC43, rhinovirus A/B/C, influenza virus A and B, respiratory syncytial virus A and B, bocavirus 1/2/3/4, human metapneumovirus, and enterovirus. The PCR amplification was performed as previously described (Ogawa et al., 2019 (link)). Agarose gel electrophoresis with ethidium bromide staining was used to determine the presence of PCR products.
Kawamura M., Hoshina T., Ogawa M., Yamamoto N., Haro K., Kumadaki T., Fukuda K, & Kusuhara K. (2020). The optimal duration of antimicrobial therapy for lower respiratory tract infection in patients with neuromuscular disorders based on a clone library analysis of the bacterial 16S rRNA gene sequence. International Journal of Infectious Diseases, 100, 396-401.
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2

Detecting Respiratory Viruses via Multiple Techniques

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Influenza virus,3 rhinovirus, and other respiratory viruses (coxsackievirus A and B4; echovirus4; enterovirus4; human coronavirus 229E, HKU1, NL63, and OC43; human metapneumovirus; human parainfluenza virus 1, 2, 3, and 4; human parechovirus5; human respiratory syncytial virus; human adenovirus6, 7; human bocavirus; human parvovirus B198; herpes simplex virus type 19; and varicella‐zoster virus10) were detected by using virus isolation, PCR, RT‐PCR, real‐time RT‐PCR, sequencing, FTD Respiratory pathogens 21 (Fast Track Diagnostics), or Seeplex RV15 OneStep ACE Detection (Seegene) as shown in Table 1.
Takashita E., Kawakami C., Momoki T., Saikusa M., Shimizu K., Ozawa H., Kumazaki M., Usuku S., Tanaka N., Okubo I., Morita H., Nagata S., Watanabe S., Hasegawa H, & Kawaoka Y. (2021). Increased risk of rhinovirus infection in children during the coronavirus disease‐19 pandemic. Influenza and Other Respiratory Viruses, 15(4), 488-494.
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3

Viral Pneumonia Diagnosis via Multiplex PCR

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Data of bronchoscopies at Ryukyu University Hospital between August 2012 and September 2016 were reviewed, and all patients who underwent multiplex PCR tests for respiratory viruses in bronchoscopy specimens were retrospectively recruited. PCR tests were performed using commercially available multiplex PCR kits (Seeplex RV15 OneStep ACE Detection, Seegene, Korea). These can detect 15 respiratory viruses (adenovirus, coronaviruses 229E/NL63 and OC43, parainfluenza viruses 1–4, rhinovirus A/B/C, respiratory syncytial viruses A and B, metapneumovirus, enterovirus, influenza A and B viruses, and bocavirus 1/2/3/4). The PCR results, diagnoses of lung lesions before considering PCR results, and medical histories were extracted for descriptive statistics. Diagnosis of pure viral pneumonia was based on the description in the medical records (no criteria for pure viral pneumonia in this study).
Nabeya D., Kinjo T., Ueno S., Setoguchi M., Nishiyama N., Kami W., Arakaki W., Haranaga S, & Fujita J. (2022). Characteristics of patients with viral infections of the lower respiratory tract: A retrospective study. Medicine, 101(38), e30819.
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4

Multiplex RT-PCR for Respiratory Viruses

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After isolation of viral DNA and RNA from viral transport medium, which was performed using the RibospinTM vRD Kit (GeneAll Biotechnology, Seoul, Korea), multiplex RT-PCR for 15 respiratory viruses, including adenovirus (AdV), human coronavirus (HCoV) 229E/Nl63, HCoV OC43, parainfluenza virus (PIV) types 1-4, RV groups A/B/C, RSV type A and B, influenza (Flu) type A and B, human bocavirus (HBoV), human metapneumovirus (HMPV), and human enterovirus (HEV), was performed using the Seeplex® RV15 OneStep ACE Detection (Seegene Inc., Seoul, Republic of Korea). Amplification was performed with a thermal cycler GeneAmp® 9700 PCR System (Applied Biosystems, Foster City, United States). Detection of PCR products was done by microchip electrophoresis on the MCE®-202 MultiNA device (Shimadzu, Kyoto, Japan). Seeplex® RV15 OneStep ACE Detection includes two internal controls: one is PCR control, in order to check the presence of substances that may interfere with PCR amplification. The other control is called the whole process control (WPC) and includes the human RNase P target as an internal control to check the entire experimental process from nucleic acid extraction to RT-PCR.
Mijač M., Ljubin-Sternak S., Ivković-Jureković I, & Vraneš J. (2023). Comparison of MT-PCR with Quantitative PCR for Human Bocavirus in Respiratory Samples with Multiple Respiratory Viruses Detection. Diagnostics, 13(5), 846.
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