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2 protocols using chromium 3 nitrate nonahydrate

1

Synthesis of Diverse Metal-Doped Silica Nanoparticles

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Tetraethyl orthosilicate (TEOS, 99.0%, Aldrich), ammonia solution (NH3·H2O, 25–28 wt%, Aldrich), ferric chloride (FeCl3, 99.0%, Macklin), cobaltous chloride hexahydrate (CoCl2·6 H2O, 99.0%, Macklin), manganese chloride tetrahydrate (MnCl2·4 H2O, 99.0%, Macklin), magnesium chloride (MgCl2·6 H2O, 99.0%, Macklin), barium chloride (BaCl2, 99.0%, Macklin), nickel chloride hexahydrate (NiCl2·6 H2O, 99.0%, Macklin), lithium chloride hydrate (LiCl·H2O, 99.0%, Macklin), chromium(III) nitrate nonahydrate (Cr(NO3)3·9 H2O, 99.99%, Macklin), molybdenum chloride (MoCl5, 99.5%, Macklin), silver chloride (AgCl, 99.99%, Macklin), chloroauric (III) acid tetrahydrate (HAuCl4·4 H2O, 99.0%, Sinopharm) and sodium borohydride (NaBH4, 99.5%, Sigma-Aldrich), were used as received. Deionized water (DI) used in the experiments was purified through a Pure-lab Prima system to a resistivity of 18.2 MΩ·cm−1.
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2

Synthesis of Multifunctional Nanoparticles

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Zinc nitrate hexahydrate (Zn(NO3)3•6H2O, 99.95%), gallium nitrate hydrate (Ga(NO3)3•xH2O, 99.99%) and chromium (III) nitrate nonahydrate (Cr(NO3)3•9H2O, 99%) were purchased from Macklin, China. Poly(vinylpyrrolidone) (PVP, Mw = 1,300,000), poly(sodium 4 styrenesulfonate) (PSS, MW=70,000) and branched Poly(ethyleneimine) (PEI, MW = 25 kDa) were supplied by Sigma-Aldrich. Sodium borohydride (NaBH4), silver nitrate (AgNO3), and Lascorbic acid (AA) were purchased from Sinopharm Chemical, China. beta-actin and hTERT antibodies were purchased from Sangon biotech. GoldView TM was supplied by SBS genetech Co., Ltd. siRNA samples were synthesized by GenePharma Company, Shanghai, China (Table 1). All other regents were obtained from Beyotime® Biotechnology. A purpose-designed LED light source was Please do not adjust margins Please do not adjust margins manufactured for in-vitro studies with the CoBs supplied by Philips Lumileds (Fig. S1).
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