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Pll3.7 ef eyfp yap1 wt polya

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PLL3.7-EF-EYFP-YAP1_WT-PolyA is a plasmid construct containing the EYFP (enhanced yellow fluorescent protein) gene fused to the wild-type YAP1 (Yes-associated protein 1) gene. The construct is driven by the EF1-alpha (Elongation Factor 1-alpha) promoter and includes a PolyA termination sequence.

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Lab products found in correlation

Pqcxih myc yap, by Addgene (2 mentions) Pqcxih flag yap s127a, by Addgene (2 mentions) Pcdna3 icue3, by Addgene (2 mentions) Gfp pki nls, by Addgene (2 mentions) Pqcxih myc yap 5sa, by Addgene (2 mentions) Pqcxih flag yap s381a, by Addgene (2 mentions) Pcw57, by Addgene (2 mentions)

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YAP1 wt (2 citations)

2 protocols using pll3.7 ef eyfp yap1 wt polya

1

Plasmids from Addgene for YAP Signaling

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The following plasmids were obtained from Addgene: pCW57.1 (Addgene plasmid, #41393); pcDNA3-ICUE3 (Addgene plasmid, # 61622) (DiPilato and Zhang, 2009 (link)). GFP-PKI nls (Addgene plasmid, #118301) (Billiard et al., 2001 (link)); pLL3.7-EF-EYFP-YAP1_WT-PolyA (Addgene plasmid, #112284) (Ege et al., 2018 (link)); pQCXIH-Myc-YAP (Addgene plasmid, #33091) (Zhao et al., 2007 (link)); pQCXIH-Myc-YAP-5SA (Addgene plasmid, #33093) (Zhao et al., 2007 (link)); pQCXIH-Flag-YAP-S127A (Addgene pasmid, # 33092) (Zhao et al., 2007 (link)); and pQCXIH-Flag-YAP-S381A (Addgene plasmid, #33068) (Zhao et al., 2010 (link)). Of note, YAP S381 in mice corresponds to residue S397 in humans; all reference to phosphorylation at this site was described as S397.
Drozdz M.M., Doane A.S., Alkallas R., Desman G., Bareja R., Reilly M., Bang J., Yusupova M., You J., Eraslan Z., Wang J.Z., Verma A., Aguirre K., Kane E., Watson I.R., Elemento O., Piskounova E., Merghoub T, & Zippin J.H. (2022). A nuclear cAMP microdomain suppresses tumor growth by Hippo pathway inactivation. Cell reports, 40(13), 111412.
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2

Plasmids from Addgene for YAP Signaling

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The following plasmids were obtained from Addgene: pCW57.1 (Addgene plasmid, #41393); pcDNA3-ICUE3 (Addgene plasmid, # 61622) (DiPilato and Zhang, 2009 (link)). GFP-PKI nls (Addgene plasmid, #118301) (Billiard et al., 2001 (link)); pLL3.7-EF-EYFP-YAP1_WT-PolyA (Addgene plasmid, #112284) (Ege et al., 2018 (link)); pQCXIH-Myc-YAP (Addgene plasmid, #33091) (Zhao et al., 2007 (link)); pQCXIH-Myc-YAP-5SA (Addgene plasmid, #33093) (Zhao et al., 2007 (link)); pQCXIH-Flag-YAP-S127A (Addgene pasmid, # 33092) (Zhao et al., 2007 (link)); and pQCXIH-Flag-YAP-S381A (Addgene plasmid, #33068) (Zhao et al., 2010 (link)). Of note, YAP S381 in mice corresponds to residue S397 in humans; all reference to phosphorylation at this site was described as S397.
Drozdz M.M., Doane A.S., Alkallas R., Desman G., Bareja R., Reilly M., Bang J., Yusupova M., You J., Eraslan Z., Wang J.Z., Verma A., Aguirre K., Kane E., Watson I.R., Elemento O., Piskounova E., Merghoub T, & Zippin J.H. (2022). A nuclear cAMP microdomain suppresses tumor growth by Hippo pathway inactivation. Cell reports, 40(13), 111412.
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