Microglial RNA was extracted using the RNeasy mini extraction kit with on-column DNase treatment (QIAGEN). RNA quality was determined with the Qubit RNA HS assay. RNA quality was checked via RIN on an Agilent Bioanalyzer 2100 with the Eukaryote Total RNA Nano chip. Libraries were generated with the Illumina RNA-seq library prep for low input RNA. Libraries were sequenced on paired-end, 75 bp runs on the Nextseq 500 (Illumina). RNA QC, library preparation, and sequencing were performed at the University of Florida’s Interdisciplinary Center for Biotechnology Research sequencing core.
Rna seq library prep
Manufactured by Illumina
The RNA-seq library prep is a laboratory equipment product designed for the preparation of RNA sequencing libraries. It enables the conversion of RNA samples into a format suitable for next-generation sequencing analysis.
Automatically generated - may contain errors
2 protocols using rna seq library prep
1
Microglial RNA Extraction and Sequencing
2
RNA-seq on Transgenic CRND8 Mice
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Microglial RNA was extracted using the RNeasy mini extraction kit with on-column DNase treatment (Qiagen). RNA quality was determined with the Qubit RNA HS assay. RNA quality was checked via RIN on an Agilent Bioanalyzer 2100 with the Eukaryote Total RNA Nano chip. Libraries were generated with the Illumina RNA-seq library prep for low input RNA. Libraries were sequenced on paired-end, 75 bp runs on the Nextseq 500 (Illumina). RNA QC, library preparation and sequencing were performed at the University of Florida's Interdisciplinary Center for Biotechnology Research (ICBR) sequencing core.
Transgenic CRND8 RNA-sequencing data Data for the transgenic CRND8 mice was obtained from the AMP-AD Knowledge Portal (doi: 10.7303/syn3157182). Experimental details are located within the data portal's website. BAM files were downloaded from the AD Knowledge portal and used with the analysis method described below. Animal numbers are as follows: 3-month, nTg-F: 6; 3-month, nTg-M: 6; 3-month, Tg-F: 6; 3month, Tg-M: 6; 6-month, nTg-F: 5; 6-month, nTg-M: 7; 6-month, Tg-F: 5; 6-month, Tg-M: 6; 12-month, nTg-F: 5; 12-month, nTg-M: 5; 12-month, Tg-F: 7; 12-month, Tg-M: 7; 20-month, nTg-F:11; 20-month, nTg-M: 5; 20-month, Tg-F: 5; 20-month, Tg-M: 3. Male and female mice of the same age and genotype were grouped together for this analysis.
Transgenic CRND8 RNA-sequencing data Data for the transgenic CRND8 mice was obtained from the AMP-AD Knowledge Portal (doi: 10.7303/syn3157182). Experimental details are located within the data portal's website. BAM files were downloaded from the AD Knowledge portal and used with the analysis method described below. Animal numbers are as follows: 3-month, nTg-F: 6; 3-month, nTg-M: 6; 3-month, Tg-F: 6; 3month, Tg-M: 6; 6-month, nTg-F: 5; 6-month, nTg-M: 7; 6-month, Tg-F: 5; 6-month, Tg-M: 6; 12-month, nTg-F: 5; 12-month, nTg-M: 5; 12-month, Tg-F: 7; 12-month, Tg-M: 7; 20-month, nTg-F:11; 20-month, nTg-M: 5; 20-month, Tg-F: 5; 20-month, Tg-M: 3. Male and female mice of the same age and genotype were grouped together for this analysis.
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