Prednisolone

Manufactured by Selleck Chemicals

Sourced in Netherlands, France, United States, Germany

Prednisolone is a synthetic corticosteroid medication used as a laboratory reagent. It is a white to practically white, odorless, crystalline powder that is freely soluble in alcohol and in acetone, and slightly soluble in water.

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Lab products found in correlation

5 protocols using prednisolone

Evaluation of Venetoclax, Navitoclax, and S63845

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The BCL-2 inhibitor venetoclax (#S8048, Houston, TX, USA), the BCL-2/BCL-XL/BCL-W inhibitor navitoclax (#S1001), and the MCL-1 inhibitor S63845 (#S8383) were all acquired from Selleckchem. CHOP chemotherapy consisted of cyclophosphamide (University Medical Center Groningen (UMCG) pharmacy, Groningen, The Netherlands), doxorubicin (#S1208, Selleckchem, Houston, TX, USA), vincristine (UMCG pharmacy, Groningen, the Netherlands), and prednisolone (#S1737, Selleckchem), in a composition set at the clinical ratio of 83/5.5/0.16/11.1, respectively (12).

de Jong M.R., Langendonk M., Reitsma B., Nijland M., van den Berg A., Ammatuna E., Visser L, & van Meerten T. (2019). Heterogeneous Pattern of Dependence on Anti-Apoptotic BCL-2 Family Proteins upon CHOP Treatment in Diffuse Large B-Cell Lymphoma. International Journal of Molecular Sciences, 20(23), 6036.

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Radiotherapy and CHOP Chemotherapy Combination

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The WEE1 inhibitor AZD1775 was acquired from Selleckchem (No.S1525, Houston, TX, USA). RT was performed at a dosage from 2 to 20 Gy using a Cesium-137 source-662 keV photons (IBL 637, Cis Bio International, Gif-sur-Yvette, France) and metabolic activity of cells was measured after 72 h. CHOP included cyclophosphamide (University Medical Center Groningen [UMCG] pharmacy), doxorubicin (No.S1208, Selleckchem), vincristine (UMCG pharmacy) and prednisolone (No.S1737, Selleckchem), in a composition set at the clinical ratio of 83/5.5/0.16/11.1, respectively.^{16 (link)}

de Jong M.R., Langendonk M., Reitsma B., Herbers P., Lodewijk M., Nijland M., van den Berg A., Ammatuna E., Visser L, & van Meerten T. (2020). WEE1 inhibition synergizes with CHOP chemotherapy and radiation therapy through induction of premature mitotic entry and DNA damage in diffuse large B-cell lymphoma. Therapeutic Advances in Hematology, 11, 2040620719898373.

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Glucocorticoid and Chemical Reagent Sourcing

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Glucocorticoids methylprednisolone and triamcinolone were purchased from MCE (New Jersey, USA). Dexamethasone, hydrocortisone, and prednisolone were purchased from SELLECK (Houston, TX, USA). Chemical triciribine was obtained from SELLECK. Kinetin riboside, cilomilast, rigosertib, PMA, and LPS were purchased from MCE Co. IFN-γ was from PeproTech (New Jersey, USA).

Xiang Z., Liu J., Shi D., Chen W., Li J., Yan R., Bi Y., Hu W., Zhu Z., Yu Y, & Yang Z. (2020). Glucocorticoids improve severe or critical COVID-19 by activating ACE2 and reducing IL-6 levels. International Journal of Biological Sciences, 16(13), 2382-2391.

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Compound Dissolution for Biological Assays

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Prednisolone, MK2206, Uprosertib and SCH772984 were purchased from Selleckchem (Munich, Germany) and dissolved as instructed by the manufacturer.

Butler M., Vervoort B.M., van Ingen Schenau D.S., Jongeneel L., van der Zwet J.C., Marke R., Meijerink J.P., Scheijen B., van der Meer L.T, & van Leeuwen F.N. (2022). Reversal of IKZF1-induced glucocorticoid resistance by dual targeting of AKT and ERK signaling pathways. Frontiers in Oncology, 12, 905665.

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Investigating Cell Line Responses to Steroid and Epigenetic Treatments

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MDA-MB-231, BT549, BT20, HCC-1937, MDA-MB-453, and Cos-7 cells were cultured with specific medium and 10% FBS at 37°C and in 5% CO₂. Before experiments, cells were grown in phenol red-free medium supplemented with 10% charcoal-stripped serum (Biowest).
When indicated, cells were treated with 100 nM Dex (Sigma-Aldrich), 100 nM prednisolone (Selleckchem), 1 μM hydrocortisone (Selleckchem), 1 μM RU486 (Selleckchem), or with 0.5 μM PRMT5 inhibitor GSK3326595 called GSK595 (Selleckchem) for the indicated time.
SMART-pool siRNAs (Dharmacon) used for the depletion of GR, HP1γ, PRMT5, G9a, GLP, and siNS were transfected into indicated cells using Lipofectamine siRNAi max (Invitrogen) according to the manufacturer’s protocol.

Noureddine L.M., Ablain J., Surmieliova-Garnès A., Jacquemetton J., Pham T.H., Marangoni E., Schnitzler A., Bieche I., Badran B., Trédan O., Hussein N., Le Romancer M, & Poulard C. (2023). PRMT5 triggers glucocorticoid-induced cell migration in triple-negative breast cancer. Life Science Alliance, 6(10), e202302009.

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